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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

Product#: DCP-MEM-QP1X
$44.00
DCP-MEM-QP1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Without L-Glutamine Without Sodium Pyruvate

FluxMPS™ DCP-MEM-QP1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA) formulated without L-Glutamine or Sodium Pyruvate for independent, defined-timepoint supplementation. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • MEM with Earle’s Salts — physiological ionic balance formulated for primary fibroblasts, neurons, epithelial and vascular cells
  • Non-Essential Amino Acids (NEAA) pre-loaded — reduces de novo synthesis burden and metabolic demand on primary cells in low-serum conditions
  • Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high-glucose-induced ROS and glycation
  • Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>) — suited to endotoxin-sensitive primary cell cultures
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Manufactured under an ISO 13485:2016 quality management system; final QA at the Diagnocine R&D Center, Totowa, NJ, USA
  • L-Glutamine and Sodium Pyruvate excluded to give researchers independent control over nitrogen and secondary carbon inputs for metabolic flux studies
  • Custom formulation modifications (pH, glucose, NEAA levels, HEPES) available on request — contact support@diagnocine.com
DCP-MEM-QP1X | Size: 500 mL and 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
  • BaseMEM + Earle’s Salts + NEAA
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • Formulation[+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose
    [-] L-Glutamine, [-] Sodium Pyruvate
  • AppearanceOrange-to-red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290 - 330 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Sterility (USP <71>)No growth / 14 days
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle’s Salts is a foundational medium for primary cell biology — but conventional 0.22 µm–filtered MEM passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that can alter primary cell gene expression, activation state, and morphology. FluxMPS™ is built to reduce these risk factors while preserving the full MEM + NEAA nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion in neuronal, epithelial, and fibroblast chip architectures.

biotech

Primary cell-optimized formulation

MEM + Earle’s Salts + NEAA is a long-established culture base for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.

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Ultrapure-grade water

Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm), supporting low trace-metal and organic carbon (TOC) content consistent with sensitive primary cell applications.

shield

Low-endotoxin release specification

Released at < 0.05 EU/mL endotoxin (LAL, USP <85>) — a specification set well below levels associated with TLR4-mediated inflammatory signaling, supporting stable fibroblast activation state and neuronal culture phenotype.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation from NEAA synthesis pathways.

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Customization on demand

pH, glucose, NEAA concentrations, HEPES, and other nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reach a final 0.04 µm polish. For primary cell culture, sub-mycoplasma filtration is especially important: mycoplasma-sized contaminants in MEM have been associated with altered cytokine profiles, metabolic shifts, and gene expression changes that can persist undetected for extended periods.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge from fouling.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates — including material in the mycoplasma size range (0.2–0.3 µm) — that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge in the train.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill and finish.

Performance vs. conventional MEM

5×
Cleaner than 0.22 µm MEM by particulate count
0.04
µm final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma is addressed through the product’s 0.1 µm mycoplasma-retentive filtration stages (not tested per lot as a distinct assay).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS(TM) DCP-MEM-QP1X Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM Earle Salts NEAA for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for sub-mycoplasma purity in primary cell OoC applications.
© Diagnocine® — DCP-MEM-QP1X
Applications

Primary cell models & OoC applications

FluxMPS™ DCP-MEM-QP1X is built for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s salt-balanced conditions are combined with 0.04 µm filtration purity for particle-free chip perfusion.

Automated Bioreactors & Robotics

Next-Generation Primary Cell Perfusion

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor perfusion of primary cell cultures where trace particulates accelerate chip fouling.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from primary cell perfusion circuits
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate neuronal and epithelial chip geometries
  • Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.

Primary neuronsiPSC-NeuronsDRG neuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical culture base for primary dermal, lung, and cardiac fibroblasts; NEAA reduces glutamine-driven ammonia accumulation associated with myofibroblast differentiation artefacts.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM reduces particulate clogging risk in sub-100 µm neuronal and epithelial chip microchannels compared with standard 0.22 µm filtered MEM.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate MEM supports confocal, TEER sensor, and biosensor workflows on primary cell chips; a phenol red-free variant is available on request for applications requiring reduced background fluorescence.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA requests: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose | [-] L-Glutamine, [-] Sodium Pyruvate
Appearance Orange-to-red colored, clear solution
Base MEM + Earle’s Salts + NEAA
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
pH USP <791> 7.4
Osmolality USP <785> 290 - 330 mOsm/kg H2O
Total ingredients 35
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 required (sodium bicarbonate buffering, 2200 mg/L)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM with Earle’s Salts and NEAA: 35 ingredients, verified per lot with CAS numbers. The Amino Acids tab includes both essential amino acids (EAA) and Non-Essential Amino Acids as one combined group, per the source composition table.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 11.000
Custom formulation: NEAA concentrations, HEPES addition, glucose level, and pH adjustments available on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular attention to primary cell-grade purity standards.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water supports low trace-metal and organic carbon content in the finished medium.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; a Certificate of Analysis is issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL; assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290 - 330 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEM-QP1X compares

FluxMPS™ DCP-MEM-QP1X vs. conventional 0.22 µm-filtered MEM and standard high-glucose DMEM for primary cell OoC applications.

Parameter DCP-MEM-QP1X (FluxMPS™) Conventional MEM (0.22 µm filtered) Standard DMEM HG (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
MEM with Earle’s Salts and NEAA, without L-Glutamine and Sodium Pyruvate check_circle Yes cancel No cancel No
Salt formulation Earle’s Salts (5% CO2 formulation) Earle’s Salts Modified Earle’s
NEAA included check_circle Yes (Ala, Asn, Asp, Glu, Pro) Optional add-on cancel Not included
Glucose 1.0 g/L (Low) 1.0 g/L 4.5 g/L (High)
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle 0.1 µm mycoplasma-retentive stages cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Ultrapure Type 1, 18.2 MΩ·cm Not specified Not specified
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Higher clogging risk cancel Higher clogging risk
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-QP1X — MEM with Earle’s Salts and NEAA.

Yes. DCP-MEM-QP1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels suited to MPS, OoC, and LoC platforms. MEM with Earle’s Salts and NEAA is well suited to primary cell OoC models — neuronal chips, epithelial chips, fibroblast chips — where low glucose and NEAA supplementation reflect a more physiological nutrient environment than high-glucose DMEM.
The 0.04 µm stages also retain material in the mycoplasma size range (0.2–0.3 µm), which a 0.22 µm filter does not.
Both are omitted to allow independent fresh supplementation, giving researchers control over nitrogen (glutamine) and secondary carbon (pyruvate) inputs separately. This supports metabolic flux studies in primary cells and neurons, where both components contribute to TCA cycle inputs; adding them at defined concentrations and timepoints enables more precise flux measurements. NEAA (alanine, asparagine, aspartate, glutamate, proline) remains pre-loaded for basal non-essential nitrogen support and does not need to be added separately.
Yes. DCP-MEM-QP1X uses MEM with Earle’s Salts and sodium bicarbonate buffering (2200 mg/L), formulated for standard 5% CO2 incubation, consistent with the product’s pH 7.4 specification. Hanks’ Salts-based formulations, by contrast, omit bicarbonate and are designed for CO2-independent culture; this variant is not formulated that way.
Yes. Serum, growth factors, and other protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm filter for serum-containing additions, since membranes at that pore size retain much of the protein and lipoprotein fraction serum is intended to provide. Note that DCP-MEM-QP1X already includes NEAA, which reduces the need for additional non-essential amino acid supplementation in most primary cell applications.
DCP-MEM-QP1X carries a release specification of < 0.05 EU/mL, verified by LAL assay per USP <85>, with an assay sensitivity of 0.005 EU/mL. Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification before product ships. A Certificate of Analysis reporting the batch result is available on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432-437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117-129. doi:10.1016/j.biomaterials.2018.07.014
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446-452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j

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