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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEM-QBR1X
$44.00
DCP-MEM-QBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-MEM-QBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA formulation engineered for primary fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Also containing Earle's Salts and non-essential amino acids (NEAA), this 1X liquid medium is formulated without L-Glutamine, Sodium Bicarbonate, and Phenol Red for CO2-independent, low-background primary cell applications. NEAA pre-loaded — reduces metabolic burden on primary cells.

  • Low Glucose (1.0 g/L) — a physiological carbon source for primary cells sensitive to high-glucose stress.
  • NEAA pre-loaded, reducing de novo synthesis burden and ammonia accumulation in primary fibroblasts, neurons, and epithelial cells.
  • Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish.
  • Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>).
  • Formulated without L-Glutamine, Sodium Bicarbonate, and Phenol Red for CO2-independent, low-background primary cell applications.
  • Manufactured under an ISO 13485:2016 quality management system, with per-lot Certificate of Analysis.
  • Custom pH, glucose concentration, and HEPES supplementation available on request.
DCP-MEM-QBR1X | Size: 500 mL and 1000 mL | Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA (Earle's Salts)
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • NEAAPresent
  • Formulation[+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate
    [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
  • AppearanceColorless, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Shelf life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a primary cell biologist's medium of choice — but conventional 0.22 µm filtered MEM passes mycoplasma-scale particles, subvisible particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ addresses these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Low-particulate primary cell media helps prevent chip channel clogging in narrow microfluidic geometries.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) is produced under controlled trace-metal and total organic carbon (TOC) limits, supporting consistent lot-to-lot primary cell culture performance.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin release specification — below the TLR4 activation threshold. In primary cell models, this specification limits the risk of LPS-driven fibroblast activation, neuroinflammatory gene expression, and epithelial barrier disruption.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

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Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — a repeated 0.1 µm prefilter and 0.04 µm final-filter pair, run twice — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates, including the 0.2–0.3 µm size range associated with mycoplasma, that pass a standard 0.22 µm filter. Mycoplasma control is by 0.1 µm/0.04 µm filtration; it is not tested per lot.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm mycoplasma-retentive filtration through the quadruple-stage train; mycoplasma testing is not performed per lot.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEM-QBR1X Minimum Essential Medium (MEM) Low Glucose NEAA w/o L-Glutamine Sodium Bicarbonate Phenol Red 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM Low Glucose plus NEAA for organ-on-a-chip and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-QBR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEM-QBR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic liquid handling — a separate, higher tier from the 0.04 µm Microfluidics Suitable product described on this page (see the Grade note in the Filtration section above).

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates.
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries.
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture.

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically closer base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity studies in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate accumulation in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Phenol red–free formulation and 0.04 µm filtration reduce background particulate for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Calcium, [+] Magnesium, [+] Glucose (1000 mg/L), [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red (also contains Earle's Salts and NEAA — see Composition tab)
Appearance Colorless, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 34 components across 4 formulation categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent; supplement with HEPES (15–25 mM) for pH buffering
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)

Pack sizes: 500 mL, 1000 mL. Contact support@diagnocine.com for other volumes or custom formulations.

Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA: 34 ingredients verified per lot with CAS numbers where known. NEAA is listed under AMINO ACIDS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
Sodium Pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-MEM-QBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, produced under controlled trace-metal and TOC limits.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending. Certificate of Analysis available for every batch.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), by light obscuration.

Osmolality — USP <785>

Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEM-QBR1X compares

FluxMPS™ DCP-MEM-QBR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEM-QBR1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM (0.22 µm)
Grade Microfluidics Suitable (0.04 µm) Not applicable — 0.22 µm single-stage filtration
MEM + NEAA without L-Glutamine, Bicarbonate, and Phenol Red — CO2-independent, low-background primary cell base check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm/0.04 µm) cancel No cancel No
NEAA included check_circle Yes Optional add-on cancel No
Endotoxin (release specification, USP <85>) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes — Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-QBR1X.

Yes. DCP-MEM-QBR1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size. MEM + Earle's Salts + NEAA, filtered to sub-mycoplasma-scale purity, with an endotoxin release specification < 0.05 EU/mL, is suitable for primary neuronal, epithelial, fibroblast, and vascular OoC platforms.
FluxMPS™ uses four sequential filters: 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish).
This formulation is CO2-independent (no sodium bicarbonate; add HEPES 15–25 mM for pH buffering), has reduced background fluorescence (no phenol red), and uses researcher-defined nitrogen supplementation (add L-glutamine, typically 2 mM, fresh at time of use). Sodium pyruvate is already included in the formulation. This combination suits open-top fluorescence-detection chips with primary neurons or epithelial cells where CO2 incubator access is limited.
No. This formulation is CO2-independent; supplement with HEPES (15–25 mM) for pH buffering outside a CO2 incubator.
Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition. Defined, protein-free supplements may be filtered at 0.1 µm. Do not use a 0.04 µm membrane for supplement filtration — it retains serum proteins, lipoproteins, and growth factors and will clog rapidly.
DCP-MEM-QBR1X is produced to a batch release specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch, with every batch tested before release. A Certificate of Analysis for each lot is available on request.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA formulations in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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