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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Phenol Red: 1X Liquid

Product#: DCP-MEM-QR1X
$44.00
DCP-MEM-QR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Phenol Red

FluxMPS™ DCP-MEM-QR1X is a Microfluidics Suitable, ultra-filtered Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA) formulation engineered for primary cell culture on organ-on-a-chip (OoC), neuronal chip, and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulated with Earle’s Salts, NEAA, Low Glucose (1000 mg/L), Sodium Pyruvate, and Sodium Bicarbonate; supplied without L-Glutamine and without Phenol Red for fresh nitrogen control and an imaging-clean optical baseline.

  • MEM with Earle’s Salts — physiological ionic balance optimized for primary fibroblasts, neurons, epithelial, and vascular cells
  • NEAA pre-loaded (Ala, Asn, Asp, Glu, Pro) — reduces de novo synthesis burden and ammonia accumulation in primary cells
  • Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high glucose-induced ROS and glycation
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish for sub-mycoplasma-range purity
  • Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>), controlled per manufacturing batch
  • Supplied without L-Glutamine (add fresh at 2 mM at use) and without Phenol Red for an imaging-clean optical baseline
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom formulation modifications (pH, glucose, NEAA, HEPES) available on request
DCP-MEM-QR1X Size: 500 mL, 1000 mL Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine, Phenol Red: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-GlutamineNot added (add fresh, 2 mM at use)
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290–330 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (gel ice packs, insulated packaging)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle’s Salts is the primary cell biologist’s medium — but conventional 0.22 µm–filtered MEM passes mycoplasma-range particles, subvisible particulates, and endotoxin fragments that cause subtle but significant alterations to primary cell gene expression, activation state, and morphology. FluxMPS™ is built to a finer 0.04 µm final cut-off while preserving the full MEM + NEAA nutritional profile that primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance supports safe perfusion in neuronal, epithelial, and fibroblast chip architectures.

biotech

Primary cell-optimized formulation

MEM + Earle’s Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) processed for low trace-metal and organic carbon (TOC) content, supporting consistent primary cell culture performance lot to lot.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL release specification (LAL assay, USP <85>) — minimizes the risk of LPS-driven inflammatory activation that can confound primary cell phenotype, fibroblast-to-myofibroblast transition, and neuronal inflammatory responses.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, improving viability in low-serum conditions and reducing ammonia accumulation from NEAA synthesis pathways.

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Customization on demand

pH, glucose, NEAA concentrations, HEPES, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish. For primary cell culture, sub-mycoplasma-range filtration is especially important — mycoplasma contamination in MEM causes altered cytokine profiles, metabolic shifts, and gene expression changes that can persist undetected for months.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge. 0.1 µm is also the validated mycoplasma-retentive grade (A. laidlawii challenge).

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-range material (0.2–0.3 µm) that would pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated 0.1 µm prefilter protects the second 0.04 µm cartridge, providing full redundancy for the mycoplasma-retentive barrier.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate 0.04 µm polishing filter; aseptic fill & finish under validated ISO Class 5 (Class 100) conditions.

Performance vs. conventional MEM

5×
Cleaner than 0.22 µm MEM by particulate count
0.04
µm Final pore size — sub-mycoplasma-range polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved via 0.1 µm / 0.04 µm mycoplasma-retentive filtration at every production stage (not tested per lot by USP <63>).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEM-QR1X Minimum Essential Medium (MEM), Low Glucose, NEAA without L-Glutamine, Phenol Red: 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I (mycoplasma-retentive), 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM Earle Salts NEAA for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) delivering a 0.04 µm final polish for sub-mycoplasma-range purity in primary cell OoC applications.
© Diagnocine® — DCP-MEM-QR1X
Applications

Primary cell models & OoC applications

FluxMPS™ DCP-MEM-QR1X is purpose-built for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s salt–balanced conditions better mimic the physiological microenvironment than DMEM — combined with 0.04 µm filtration for particle-free chip perfusion.

Automated Bioreactors & Robotics

Next-Generation Primary Cell Perfusion

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor perfusion of primary cell cultures where trace particulates cause accelerated chip fouling.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from primary cell perfusion circuits
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate neuronal and epithelial chip geometries
  • Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.

Primary neuronsiPSC-NeuronsDRG neuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical culture base for primary dermal, lung, and cardiac fibroblasts — NEAA reduces glutamine-driven ammonia accumulation that can promote myofibroblast differentiation artefacts.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM reduces particulate clogging risk in sub-100 µm neuronal and epithelial chip microchannels compared with standard 0.22 µm filtered MEM.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate MEM; the phenol red-free formulation reduces spectral interference, and 0.04 µm filtration minimizes background particulate scatter for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every batch released against the full specification matrix. CoA: support@diagnocine.com.

Available pack sizes: 500 mL, 1000 mL.

Physical & Chemical Parameters
Parameter Specification
Base MEM + Earle’s Salts + NEAA
Formulation [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red
Appearance Pale yellow-colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
pH USP <791> 7.4
Osmolality USP <785> 290–330 mOsm/kg H2O
Total ingredients 35 across 3 composition groups (Inorganic Salts; Amino Acids; Vitamins & Others)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification — see § Manufacturing & Compliance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack (gel ice packs, insulated packaging)
CO2 requirement Approximately 6% CO2 (derived from 2200 mg/L NaHCO3 at pH 7.4; validate for your application)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM with Earle’s Salts and NEAA: 35 ingredients verified per batch with CAS numbers.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: NEAA concentrations, HEPES addition, glucose level, and pH adjustments available on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular care for primary cell-grade purity standards.

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ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm, low trace-metal and organic carbon (TOC) content for consistent primary cell culture performance.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every batch.

Endotoxin — USP <85> BET

Release specification < 0.05 EU/mL, controlled per manufacturing batch. See the batch-level quality control note below.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290–330 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-MEM-QR1X batch at support@diagnocine.com.
Product Comparison

How DCP-MEM-QR1X compares

FluxMPS™ DCP-MEM-QR1X vs. conventional 0.22 µm–filtered MEM and standard high-glucose DMEM for primary cell OoC applications.

Parameter DCP-MEM-QR1X (FluxMPS™) Conventional MEM
(0.22 µm filtered)		 Standard DMEM HG
(0.22 µm filtered)
MEM with Earle’s Salts, NEAA, and Sodium Pyruvate without L-Glutamine and Phenol Red — imaging-clean primary cell base with fresh nitrogen control check_circle Yes cancel No cancel No
Grade Microfluidics Suitable (0.04 µm final cut-off) Standard grade (0.22 µm) Standard grade (0.22 µm)
Salt formulation Earle’s Salts (bicarbonate-buffered) Earle’s Salts Modified Earle’s
NEAA included check_circle Yes (Ala, Asn, Asp, Glu, Pro) Optional add-on cancel Not included
Glucose 1.0 g/L (Low — physiological) 1.0 g/L 4.5 g/L (High)
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 / 0.04 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested (Method 1) check_circle Yes cancel No cancel No
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes — Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-QR1X — MEM with Earle’s Salts and NEAA.

Yes. DCP-MEM-QR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM with Earle’s Salts and NEAA is particularly suited to primary cell OoC models — neuronal chips, epithelial chips, fibroblast chips — where low-glucose, NEAA-supplemented conditions better mimic physiological nutrient environments than DMEM.
For primary cell cultures, this is especially important: mycoplasma contamination in MEM causes altered cytokine profiles, metabolic shifts, and gene expression changes that can persist undetected for months.
Phenol red is removed to reduce spectral interference for imaging and to avoid its weak estrogenic activity in hormone-sensitive primary cell assays. L-glutamine is removed for fresh supplementation (2 mM at use), since glutamine degrades in liquid storage over time. Together these omissions create a clean optical and metabolic baseline for live-cell imaging of neuronal, epithelial, or ER-expressing primary cells on OoC platforms. NEAA and sodium pyruvate provide non-essential amino acid and oxidative carbon support in place of added glutamine.
MEM with Earle’s Salts (used in DCP-MEM-QR1X) contains sodium bicarbonate and is buffered for CO2 incubator use, delivering pH 7.4 under standard tissue culture conditions (approximately 6% CO2, derived from the 2200 mg/L sodium bicarbonate concentration). Hanks’ Salts formulations omit sodium bicarbonate and are designed for CO2-independent environments. DCP-MEM-QR1X uses Earle’s Salts with sodium bicarbonate for standard CO2 incubation.
NEAA are pre-loaded, providing basal non-essential nitrogen and carbon skeleton support. This reduces the metabolic burden on primary cells that must otherwise synthesize these amino acids de novo from glutamine and glucose — improving viability in low-serum conditions, reducing ammonia from de novo NEAA synthesis, and supporting proliferation in primary cell types adapted to NEAA-supplemented environments.
DCP-MEM-QR1X carries a release specification of < 0.05 EU/mL, controlled per manufacturing batch by LAL assay (USP <85>; assay sensitivity 0.005 EU/mL). Every batch is tested before release and must meet this specification before shipment. This matters for primary cells: endotoxin concentrations above the TLR4 activation threshold can alter fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function independently of experimental conditions. A Certificate of Analysis is available for every batch on request.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control (filtration-based), particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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