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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-MEMH-PR1X
$49.50
DCP-MEMH-PR1X
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verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ DCP-MEMH-PR1X is an MPS-grade, ultra-filtered MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for Primary fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. NEAA pre-loaded — reduces metabolic burden on primary cells. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO₂-independent pH buffering. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] Sodium Pyruvate, [-] Phenol Red.

  • Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high glucose.
  • NEAA included reduces metabolic burden and ammonia from de novo synthesis
  • 25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO₂; ideal for open-air handling, flow cytometry prep, and atmospheric incubation
  • 0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin for sensitive primary and hematopoietic cell cultures
  • Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
  • Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
DCP-MEMH-PR1X | Size: 500 mL and 1000 mL| UNSPSC 41122100| Cell Culture Media
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM, pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25mM HEPES
    [-] Sodium Pyruvate, [-] Phenol Red
  • AppearancePale Yellow-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290.00-330.00 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 μm ×2 + 0.04 μm ×2
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium but conventional 0.22 μm filtered MEM passes mycoplasma, particulates, and endotoxin that alter primary cell phenotype. FluxMPS™ eliminates these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particle-free primary cell media prevents chip channel clogging and mycoplasma-driven phenotypic drift.

biotech

Primary cell?optimized formulation

MEM + Earle's Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: CO₂-stable pH

25 mM HEPES prevents pH rise during open-air suspension culture handling, flow cytometry prep, and multi-well assay setup outside CO₂ incubators.

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Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin below the TLR4 activation threshold. For primary cells, prevents LPS-driven fibroblast activation, neuroinflammation, and epithelial barrier disruption.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, improving viability in low-serum conditions and reducing ammonia accumulation.

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Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 μm Pre-filtration I — Large Particulate Removal

    Removes large aggregates; protects downstream 0.04 µm membranes.

  2. 2

    0.04 μm Pre-filtration II — Mycoplasma Barrier

    Retains mycoplasma (0.1-0.3 µm) — absent from standard 0.22 µm filtration.

  3. 3

    0.1 μm Sterile-filtration I — Second-pass Redundancy

    Second-pass sterility redundancy; no breakthrough from Stage 1.

  4. 4

    0.04 μm Sterile-filtration II — Final Polish

    Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-MEMH-PR1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered media, with confirmed mycoplasma barrier at every production stage.

5×
Cleaner than 0.22 µm media by particulate count
0.04
μm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier every lot.
FluxMPS™ DCP-MEMH-PR1X Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Pre-filtration I, 0.04 μm Pre-filtration II mycoplasma barrier, 0.1 μm Sterile-filtration I, 0.04 μm Final Polish ? MPS-grade MEM Low Glucose + NEAA + 25mM | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-PR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-PR1X is purpose-built for Primary fibroblasts and related cell models with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered variant available on request.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Eliminates micro-fouling in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is the physiological-closest base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is the classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM prevents particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate; phenol red—free variants eliminate autofluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] Sodium Pyruvate, [-] Phenol Red
Appearance Pale Yellow-colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 290.00-330.00 mOsm/kg H₂O
Total ingredients 35
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from manufacture
Shipping condition Cold pack
CO₂ requirement 5% CO₂ recommended (dual HEPES + bicarbonate buffering; HEPES alone maintains pH without CO₂)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
Regulatory alignment 21 CFR Part 820 (cGMP) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 35 ingredients verified per lot with CAS numbers. NEAA listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulphate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-PR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL per lot.

Particulate — USP <788> Method 2

≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290.00-330.00 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-MEMH-PR1X lot at support@diagnocine.com.
Product Comparison

How DCP-MEMH-PR1X compares

FluxMPS™ DCP-MEMH-PR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA + formulations.

Parameter DCP-MEMH-PR1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 μm filtered)		 Standard DMEM (0.22 μm)
MEM + NEAA + 25mM HEPES - no Pyruvate and no Phenol Red; imaging-clean with defined carbon check_circle Yes cancel No cancel No
Final filtration pore size 0.04 μm 0.22 μm 0.22 μm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier check_circle Yes (0.04 μm) cancel No cancel No
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
Endotoxin specification < 0.05 EU/mL NMT 1 EU/mL NMT 1 EU/mL
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle MPS-grade cancel Risk of clogging cancel Risk of clogging
FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-PR1X.

DCP-MEMH-PR1X is processed through a Quadruple-stage filtration system reaching 0.04 µm, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with sub-mycoplasma filtration purity is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.
FluxMPS™ uses four sequential filters ? 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish ? resulting in approximately 5× fewer particles and confirmed mycoplasma retention at every production stage.
Phenol red removed for autofluorescence-free confocal and TEER-sensor imaging; pyruvate removed for fresh supplementation. HEPES + bicarbonate provides dual-buffer pH stability. Preferred for imaging-monitored primary cell OoC platforms where pyruvate concentration is an experimental variable.
5% CO₂ recommended (dual HEPES + bicarbonate buffering; HEPES alone maintains pH without CO₂).
NEAA are pre-loaded, providing non-essential nitrogen and carbon skeleton support. This reduces the metabolic burden on primary cells that must synthesize these amino acids de novo from glutamine and glucose improving viability in low-serum conditions and reducing ammonia accumulation.
FluxMPS™ DCP-MEMH-PR1X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>). For primary cells, endotoxin activates TLR4, altering fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432-437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117-129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446-452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201?1212. doi:10.1039/c3lc41017j

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