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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-MEMH-PR1X
$49.50
DCP-MEMH-PR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-MEMH-PR1X is a Microfluidics Suitable, ultra-filtered MEM Low Glucose + NEAA + 25mM HEPES formulation with Earle's Salts, engineered for primary fibroblasts, neurons, epithelial and vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it reaches a 0.04 µm final polish for microchannel-safe, ultra-low-particulate delivery. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides robust pH buffering for open-air handling. Sodium pyruvate and phenol red are both omitted so users can supplement pyruvate at time of use and run autofluorescence-sensitive imaging assays.

  • Low Glucose (1.0 g/L, 1000 mg/L D-Glucose) — physiological carbon source for primary cells sensitive to high-glucose stress
  • NEAA pre-loaded across 20 amino acids (Gly, Ala, Asn, Asp, Cys, Glu, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val plus Gln, Arg) — reduces de novo synthesis burden on primary cells
  • 25 mM HEPES (5958 mg/L, pKa 7.3 at 37°C) — pH-stable outside CO₂ incubators; suited to open-air handling and flow cytometry prep
  • Sodium bicarbonate (2200 mg/L) retained alongside HEPES for dual-buffer capacity under standard 5% CO₂ culture
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • Sodium pyruvate and phenol red both excluded — supports autofluorescence-sensitive imaging and user-defined pyruvate supplementation
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
DCP-MEMH-PR1X | Size: 500 mL, 1000 mL | Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent (20 amino acids)
  • AppearancePale yellow, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290 - 330 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a long-standing primary cell biology base — but conventional 0.22 µm filtered MEM passes mycoplasma-sized organisms, subvisible particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ addresses these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 particulate compliance. Ultra-low particulate media reduces the risk of chip microchannel clogging and mycoplasma-related phenotypic drift in primary cell culture.

biotech

Primary cell–optimized formulation

MEM with Earle's Salts, Low Glucose and NEAA is a long-established base for primary fibroblasts, neurons, epithelial and vascular cells.

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HEPES: CO₂-stable pH

25 mM HEPES helps limit pH rise during open-air suspension handling, flow cytometry prep, and multi-well assay setup outside CO₂ incubators.

shield

Below the TLR4 activation range

Released to a < 0.05 EU/mL endotoxin specification, below the concentration typically associated with TLR4 activation — relevant to fibroblast activation state, neuroinflammatory signaling, and epithelial barrier integrity in primary cells.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, which can improve viability in low-serum conditions and reduce ammonia accumulation from amino acid biosynthesis.

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Customization on demand

pH, glucose, HEPES concentration and other formulation adjustments available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four sequential filtration passes, run as two dedicated prefilter + final-filter pairs, reach a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris and protein aggregates; a 0.1 µm mycoplasma-retentive membrane (not tested per lot). Protects the first 0.04 µm cartridge downstream.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a standard 0.22 µm filter allows through.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated 0.1 µm prefilter protecting the second 0.04 µm cartridge — this pair does not polish the first pass, it safeguards the final filter.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-MEMH-PR1X runs a validated four-pass, two-pair filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, well below the 0.22 µm pore size used for conventional sterile-filtered media.

0.04
µm final pore size
4
Sequential filtration passes
Sterility & mycoplasma control: No growth after 14-day incubation (USP <71>). Mycoplasma is controlled by 0.1 µm mycoplasma-retentive filtration; mycoplasma organisms (0.2–0.3 µm) are retained by this pore size, though the medium is not tested per lot by a dedicated mycoplasma assay.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEMH-PR1X Minimum Essential Medium MEM Low Glucose NEAA 25mM HEPES w/o Sodium Pyruvate Phenol Red 1X Liquid - Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip and microfluidic cell culture applications - Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-PR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-PR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity supporting microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered variant of this formulation — Diagnocine's separate MPS Grade line — is available on request for automated bioreactor and robotic liquid-handling systems.

  • Total particulate exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the reach of the 0.04 µm Microfluidics Suitable tier.
  • Valve & sensor protection: Helps limit micro-fouling in delicate chip and instrument geometries.
  • Extended perfusion stability: Supports consistent nutrient delivery over long-duration perfusion culture.

Inquiry required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate; phenol red–free formulation reduces background fluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Phenol Red, [-] Sodium Pyruvate
Appearance Pale yellow, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present (20 amino acids — see Full Composition)
Sodium Pyruvate Not added
Phenol Red Not added
pH USP <791> 7.4
Osmolality USP <785> 290 - 330 mOsm/kg H₂O
Total ingredients 36
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement 5% CO₂ recommended (dual HEPES + bicarbonate buffering; HEPES alone maintains pH for short open-air handling)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 36 ingredients verified per lot with CAS numbers. NEAA listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) and D-Glucose listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-PR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm feed water — minimizes trace-metal and organic (TOC) content.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending. Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), by light obscuration.

Osmolality — USP <785>

Target: 290 - 330 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMH-PR1X compares

FluxMPS™ DCP-MEMH-PR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA and standard DMEM formulations.

Parameter DCP-MEMH-PR1X (FluxMPS™) Conventional MEM Low Glucose (0.22 µm filtered) Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Standard grade Standard grade
MEM + NEAA + 25mM HEPES, no pyruvate, no phenol red (imaging-clean with defined carbon) check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-PR1X.

Yes. DCP-MEMH-PR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels suited to MPS, OoC, and LoC platforms. MEM with Earle's Salts and NEAA, with 0.1 µm mycoplasma-retentive filtration, is well suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.
FluxMPS™ uses four sequential filters run as two pairs: 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — Polish, reaching a 0.04 µm final cut-off well below the 0.22 µm pore size of conventional media.
Phenol red is removed to support autofluorescence-sensitive confocal and TEER-sensor imaging; sodium pyruvate is removed so it can be added fresh at time of use, since it degrades in stored liquid media. HEPES (25 mM) plus sodium bicarbonate provides dual-buffer pH stability. If pyruvate is required for your assay, add it directly to a final concentration matching your protocol; the base formulation already provides D-glucose (1.0 g/L) as the primary carbon source.
5% CO₂ is recommended, consistent with the sodium bicarbonate (2200 mg/L) content of this formulation. The 25 mM HEPES component provides supplemental buffering and can maintain pH during short periods of open-air handling outside a CO₂ incubator.
Yes. For serum or other protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane before adding to the base medium; a 0.2 µm pore size preserves serum proteins, lipoproteins and growth factors that a finer membrane would retain. For defined, protein-free additions, a 0.1 µm membrane is appropriate. Do not use a 0.04 µm membrane for supplement filtration.
DCP-MEMH-PR1X is released to a specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test). This is a per-batch release specification — every manufacturing batch is tested before release and must meet this limit; it is not a per-unit certificate. For primary cells, endotoxin activates TLR4, which can alter fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432-437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117-129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446-452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j

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