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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

Product#: DCP-MEMH-P1X
$49.50
DCP-MEMH-P1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Without Sodium Pyruvate

FluxMPS™ DCP-MEMH-P1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for primary fibroblasts, neurons, epithelial and vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Earle's Salts and non-essential amino acids (NEAA) are pre-loaded to reduce metabolic burden on primary cells that would otherwise synthesize these de novo, while 25 mM HEPES (pKa 7.3 at 37°C) provides robust pH stability outside a CO₂ incubator. Sodium pyruvate is withheld from the base formulation so a defined 1 mM dose can be added fresh at the point of use.

  • Low Glucose (1.0 g/L) — a physiological carbon source for primary cells sensitive to high-glucose media.
  • NEAA pre-loaded — reduces de novo synthesis burden and ammonia accumulation in primary fibroblasts, neurons and epithelial cells.
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO₂-independent pH buffering for open-air handling, flow cytometry prep and atmospheric incubation.
  • Sodium pyruvate withheld from the base formulation — add fresh at 1 mM (110 mg/L) for defined control of oxidative substrate supply.
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish for microchannel-safe purity.
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) — relevant to TLR4-sensitive primary fibroblast and neuronal cultures.
  • Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis.
  • Ultrapure Type 1 water (18.2 MΩ·cm) and ISO Class 5 aseptic fill & finish.
DCP-MEMH-P1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-Glutamine292 mg/L (~2 mM)
  • Sodium PyruvateNot added — add fresh at 1 mM if required
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290 – 330 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a foundational primary-cell medium — but conventional 0.22 µm filtered MEM passes particulates and mycoplasma-sized organisms that standard filtration does not retain. FluxMPS™ is filtered to a finer cut-off while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate testing. Low-particulate media helps prevent chip channel clogging in sub-100 µm geometries.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: a long-established base for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: CO₂-stable pH

25 mM HEPES resists pH drift during open-air suspension handling, flow cytometry prep, and multi-well assay setup outside CO₂ incubators.

shield

Below the TLR4 activation threshold

< 0.05 EU/mL endotoxin release specification — relevant for primary cells, where LPS exposure can drive fibroblast activation, neuroinflammatory signaling, and epithelial barrier disruption.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter, including organisms in the 0.2–0.3 µm mycoplasma size range.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day sterility incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm and 0.04 µm mycoplasma-retentive filtration at every stage (not tested per lot as a separate mycoplasma assay).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEMH-P1X Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? Microfluidics Suitable MEM Low Glucose + NEAA + 25mM HEPES | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-P1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-P1X is purpose-built for primary fibroblasts, neurons, epithelial and vascular cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic liquid-handling systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically closer base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity studies in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s Salts ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate media supports confocal and biosensor work; a phenol red–free variant of this formulation is available on request where autofluorescence must be minimized.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com. Available pack sizes: 500 mL, 1000 mL.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Sodium Pyruvate
Media family MEM Low Glucose + NEAA + 25mM HEPES (Earle's Salts base)
Appearance Orange-to-Red colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 290 – 330 mOsm/kg H₂O
Total ingredients 37 across 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others), presented in 3 tabs
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm & 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement 5% CO₂ recommended (HEPES + sodium bicarbonate dual buffering; HEPES maintains pH stability during CO₂-free handling)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 37 ingredients verified per lot with CAS numbers. NEAA is listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 11.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-P1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm, trace-metal and organic-carbon (TOC) controlled feed water.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), by light obscuration.

Osmolality — USP <785>

Target: 290 – 330 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMH-P1X compares

FluxMPS™ DCP-MEMH-P1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations, and against published supplier endotoxin specifications.

Parameter DCP-MEMH-P1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM (0.22 µm)
Grade Microfluidics Suitable (0.04 µm final cut-off) Not specified Not specified
MEM + NEAA + 25mM HEPES, no sodium pyruvate for defined supplementation check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration passes 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes — 0.04 µm filtered cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-P1X.

Yes. DCP-MEMH-P1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels suited to MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with mycoplasma-retentive filtration and an endotoxin release specification of < 0.05 EU/mL is well suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.
 
Sodium pyruvate is omitted so it can be added fresh at a defined concentration (1 mM / 110 mg/L), enabling precise control of oxidative substrate supply in primary cells. HEPES helps stabilize pH during the supplementation step. This formulation is preferred for primary neuronal or epithelial studies where pyruvate dose-response is an experimental variable.
5% CO₂ is recommended. The formulation uses dual HEPES and sodium bicarbonate buffering; HEPES alone can maintain pH stability for shorter periods outside a CO₂ incubator, such as during handling and imaging.
Yes. Serum and other protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — a 0.04 µm membrane will strip serum of the lipoproteins and growth factors it is meant to supply and is not recommended for this purpose. Defined, protein-free additions may use a 0.1 µm membrane.
FluxMPS™ DCP-MEMH-P1X is produced to a batch release specification of < 0.05 EU/mL by LAL assay (USP <85>), with an assay sensitivity of 0.005 EU/mL. Every batch is tested before release. For primary cells, endotoxin activates TLR4, which can alter fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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