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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Phenol Red: 1X Liquid

Product#: DCP-MEMH-R1X
$49.50
DCP-MEMH-R1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Phenol Red: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Phenol Red

FluxMPS™ DCP-MEMH-R1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for primary fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA pre-loaded — reduces metabolic burden on primary cells. HEPES (25 mM, pKa 7.3 at 37°C) provides robust pH buffering alongside sodium bicarbonate. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Calcium, [+] Magnesium | [-] Phenol Red.

  • Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high glucose.
  • NEAA included reduces metabolic burden and ammonia accumulation from de novo synthesis.
  • 25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO₂; suited to open-air handling, flow cytometry prep, and atmospheric incubation.
  • 0.04 µm final nano-filtration — 0.1 µm mycoplasma-retentive filtration stage and an endotoxin release specification of < 0.05 EU/mL for sensitive primary and hematopoietic cell cultures.
  • Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II (Polish).
  • Ultrapure Type 1 water (18.2 MΩ·cm) and ISO 13485:2016-certified manufacturing with ISO Class 5 fill & finish.
DCP-MEMH-R1X | Size: 500 mL and 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-Glutamine292 mg/L
  • Sodium Pyruvate110 mg/L
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290–330 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes mycoplasma-sized organisms, subvisible particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ is built to reduce these risks while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate primary cell media reduces the risk of chip channel clogging, and the 0.1 µm mycoplasma-retentive filtration stage helps limit mycoplasma contamination risk.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: the established standard base for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

HEPES: CO₂-stable pH

25 mM HEPES helps limit pH rise during open-air suspension culture handling, flow cytometry prep, and multi-well assay setup outside CO₂ incubators.

visibility

Low background for imaging

Ultra-low particulate baseline reduces background interference for confocal microscopy, TEER-integrated chips, and biosensor-based assays on primary cell chips.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; mycoplasma-retentive grade, retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEMH-R1X Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o Phenol Red: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? Microfluidics Suitable MEM Low Glucose + NEAA + 25mM HEPES for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-R1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-R1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for automated bioreactors and robotics platforms.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically-relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps reduce the risk of particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline supports confocal microscopy, TEER sensor integration, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Calcium, [+] Magnesium | [-] Phenol Red
Appearance Pale yellow, clear solution (phenol red–free)
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 290–330 mOsm/kg H₂O
Total ingredients 37 components across 4 categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement 5% CO₂ recommended (HEPES + sodium bicarbonate dual buffering; HEPES alone maintains pH without CO₂)
Available pack sizes 500 mL, 1000 mL
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 37 ingredients verified per lot with CAS numbers, across 4 categories. NEAA components are listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) and i-Inositol are listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-R1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — low trace-metal and organic-carbon background supports batch-to-batch consistency.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290–330 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMH-R1X compares

FluxMPS™ DCP-MEMH-R1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH-R1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Standard grade (0.22 µm filtered) Standard grade (0.22 µm filtered)
MEM + NEAA + 25mM HEPES without Phenol Red — imaging-friendly, pH-stable primary cell base for OoC check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle 0.1 µm stage cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available on request cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-R1X.

Yes. DCP-MEMH-R1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels appropriate for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with 0.1 µm mycoplasma-retentive filtration is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish) — reducing particulate counts by approximately 5× versus conventional 0.22 µm media, with 0.1 µm mycoplasma-retentive filtration integrated at every stage.
Phenol red is removed to avoid its interference in absorbance and fluorescence readouts at 520–560 nm. HEPES (25 mM) maintains pH stability during imaging sessions outside CO₂ incubators — useful for live-cell confocal imaging of primary neurons and epithelial cells, where CO₂ fluctuation during microscopy would otherwise be tracked via a phenol red color shift. Note that riboflavin (0.1 mg/L) remains in this formulation and contributes some background fluorescence independent of phenol red.
5% CO₂ is recommended. The formulation uses dual HEPES + sodium bicarbonate buffering: HEPES alone maintains pH stability without CO₂ for short open-air handling, while 5% CO₂ supports the sodium bicarbonate buffer system for standard incubator culture.
Yes. DCP-MEMH-R1X accepts serum, growth factors, and other protein-containing supplements. When adding serum or other protein-containing components, filter the supplement separately through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm membrane for protein-containing additions — it will strip essential serum proteins and clog rapidly. Defined, protein-free additions may use a 0.1 µm membrane.
The release specification is < 0.05 EU/mL, verified by LAL assay per USP <85> on every manufacturing batch before release (assay sensitivity 0.005 EU/mL). This is a batch-level specification, not a per-unit certificate. Low endotoxin matters for primary cells because endotoxin activates TLR4, which can affect fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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