FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ DCP-MEMH-QPR1X is an MPS-grade, ultra-filtered MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for Primary fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. NEAA pre-loaded — reduces metabolic burden on primary cells. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO₂-independent pH buffering. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red.

Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high glucose.
NEAA included reduces metabolic burden and ammonia from de novo synthesis
25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO₂; ideal for open-air handling, flow cytometry prep, and atmospheric incubation
0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin for sensitive primary and hematopoietic cell cultures
Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill

DCP-MEMH-QPR1X | Size: 500 mL and 1000 mL| UNSPSC 41122100| Cell Culture Media

Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Media familyMEM Low Glucose + NEAA + 25mM HEPES
Glucose1000 mg/L (1.0 g/L, Low Glucose)
HEPES25 mM, pKa 7.3 at 37°C
NEAAPresent
Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] Sodium Bicarbonate, [+] 25mM HEPES
[-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red
AppearancePale Yellow-colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)290.00–330.00 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes mycoplasma, particulates, and endotoxin that alter primary cell phenotype. FluxMPS™ eliminates these failure modes while preserving the full nutritional profile primary cells depend on.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particle-free primary cell media prevents chip channel clogging and mycoplasma-driven phenotypic drift.

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Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: CO₂-stable pH

25 mM HEPES prevents pH rise during open-air suspension culture handling, flow cytometry prep, and multi-well assay setup outside CO₂ incubators.

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Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin — below the TLR4 activation threshold. For primary cells, prevents LPS-driven fibroblast activation, neuroinflammation, and epithelial barrier disruption.

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NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, improving viability in low-serum conditions and reducing ammonia accumulation.

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Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1

0.1 µm Pre-filtration I — Large Particulate Removal

Removes large aggregates; protects downstream 0.04 µm membranes.
2

0.04 µm Pre-filtration II — Mycoplasma Barrier

Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration.
3

0.1 µm Sterile-filtration I — Second-pass Redundancy

Second-pass sterility redundancy; no breakthrough from Stage 1.
4

0.04 µm Sterile-filtration II — Final Polish

Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-MEMH-QPR1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered media, with confirmed mycoplasma barrier at every production stage.

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size — sub-mycoplasma polishing

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier every lot.

FluxMPS™ DCP-MEMH-QPR1X Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Pre-filtration I, 0.04 μm Pre-filtration II mycoplasma barrier, 0.1 μm Sterile-filtration I, 0.04 μm Final Polish ? MPS-grade MEM Low Glucose + NEAA + 25mM | Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-QPR1X

Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-QPR1X is purpose-built for Primary fibroblasts and related cell models with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered variant available on request.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Eliminates micro-fouling in delicate chip geometries
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is the physiological-closest base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip

Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip

Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is the classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts

Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM prevents particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS

Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate; phenol red–free variants eliminate autofluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Bicarbonate, [+] 25mM HEPES \| [-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red
Appearance	Pale Yellow-colored, clear solution
Glucose	1000 mg/L (1.0 g/L, Low Glucose)
HEPES	25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA	Present
pH USP <791>	7.4
Osmolality USP <785>	290.00–330.00 mOsm/kg H₂O
Total ingredients	34

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788>	NMT 25/mL
Particulate ≥25 µm USP <788>	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from manufacture
Shipping condition	Cold pack
CO₂ requirement	5% CO₂ recommended (dual HEPES + bicarbonate buffering; HEPES alone maintains pH without CO₂)

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
Regulatory alignment	21 CFR Part 820 (cGMP) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 34 ingredients verified per lot with CAS numbers. NEAA listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) listed under OTHERS.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	265.000
Magnesium sulphate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2200.000
Sodium chloride	7647-14-5	6800.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	122.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	7.500
L-Alanine	56-41-7	8.900
L-Arginine hydrochloride	1119-34-2	126.000
L-Asparagine monohydrate	5794-13-8	15.000
L-Aspartic acid	56-84-8	13.300
L-Cystine dihydrochloride	30925-07-6	31.300
L-Glutamic acid	56-86-0	14.700
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	52.000
L-Leucine	61-90-5	52.000
L-Lysine hydrochloride	657-27-2	72.500
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	32.000
L-Proline	147-85-3	11.500
L-Serine	56-45-1	10.500
L-Threonine	72-19-5	48.000
L-Tryptophan	73-22-3	10.000
L-Tyrosine disodium salt dihydrate	69847-15-0	51.900
L-Valine	72-18-4	46.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	1.000
D-Ca-Pantothenate	137-08-6	1.000
Folic acid	59-30-3	1.000
Nicotinamide	98-92-0	1.000
Pyridoxal hydrochloride	65-22-5	1.000
Riboflavin	83-88-5	0.100
Thiamine hydrochloride	67-03-8	1.000
i-Inositol	87-89-8	2.000
OTHERS
D-Glucose	50-99-7	1000.000
HEPES	7365-45-9	5958.000

Custom formulation: Contact support@diagnocine.com for DCP-MEMH-QPR1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL per lot.

Particulate — USP <788> Method 2

≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290.00–330.00 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-MEMH-QPR1X lot at support@diagnocine.com.

Product Comparison

How DCP-MEMH-QPR1X compares

FluxMPS™ DCP-MEMH-QPR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA + formulations.

Parameter	DCP-MEMH-QPR1X (FluxMPS™)	Conventional MEM Low Glucose (0.22 µm filtered)	Standard DMEM (0.22 µm)
MEM + NEAA + 25mM HEPES — no L-Glutamine, no Pyruvate, no Phenol Red; imaging-clean complete metabolic control	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier	check_circle Yes (0.04 µm)	cancel No	cancel No
HEPES (25 mM) included	check_circle Yes	cancel Usually no	cancel No
NEAA included	check_circle Yes	Optional add-on	cancel No
Endotoxin specification	< 0.05 EU/mL	NMT 1 EU/mL	NMT 1 EU/mL
USP <788> particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle MPS-grade	cancel Risk of clogging	cancel Risk of clogging

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-QPR1X.

DCP-MEMH-QPR1X is processed through a Quadruple-stage filtration system reaching 0.04 µm, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with sub-mycoplasma filtration purity is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.

FluxMPS™ uses four sequential filters — 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish — resulting in approximately 5× fewer particles and confirmed mycoplasma retention at every production stage.

No phenol red for autofluorescence-free imaging; no glutamine and pyruvate for fully researcher-defined nitrogen and carbon. HEPES + bicarbonate provides stable dual-buffer pH. Optimal for confocal or TEER-monitored primary cell OoC where all metabolic inputs must be independently controlled.

5% CO₂ recommended (dual HEPES + bicarbonate buffering; HEPES alone maintains pH without CO₂).

NEAA are pre-loaded, providing non-essential nitrogen and carbon skeleton support. This reduces the metabolic burden on primary cells that must synthesize these amino acids de novo from glutamine and glucose — improving viability in low-serum conditions and reducing ammonia accumulation.

FluxMPS™ DCP-MEMH-QPR1X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>). For primary cells, endotoxin activates TLR4, altering fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid