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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-MEMH-QPR1X
$49.50
DCP-MEMH-QPR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Without L-Glutamine Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-MEMH-QPR1X is a Microfluidics Suitable, ultra-filtered MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for primary fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded, reducing metabolic burden on primary cells. HEPES (25 mM, pKa 7.3 at 37°C) provides robust pH buffering alongside sodium bicarbonate. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red.

  • Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high-glucose media
  • NEAA pre-loaded — reduces de novo synthesis burden and ammonia accumulation from NEAA biosynthesis
  • 25 mM HEPES (pKa 7.3 at 37°C) working alongside sodium bicarbonate for stable pH during open-bench handling and flow cytometry prep
  • 0.04 µm final filtration — sub-mycoplasma pore-size range; endotoxin release specification < 0.05 EU/mL for sensitive primary and hematopoietic cultures
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 aseptic fill
  • L-Glutamine, Sodium Pyruvate and Phenol Red intentionally excluded for fully researcher-defined metabolic and imaging control
DCP-MEMH-QPR1X | Size: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] Sodium Bicarbonate, [+] 25mM HEPES
    [-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red
  • AppearancePale Yellow-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290–330 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes mycoplasma-scale particles, subvisible particulates, and higher endotoxin loads that can alter primary cell phenotype. FluxMPS™ addresses these failure modes at the filtration level while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Ultra-low particulate primary cell media reduces the risk of channel clogging in sub-100 µm chip geometries.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) minimizes trace-metal and organic-carbon (TOC) contribution to the finished medium.

visibility

Low background for imaging

The 0.04 µm final filter gives an ultra-low particulate baseline that supports confocal imaging and biosensor/TEER workflows on primary cell chips. Note: this formulation contains riboflavin, a required B-vitamin that contributes native fluorescence common to all defined media.

science

Reduced endotoxin-associated risk

Released to a < 0.05 EU/mL specification, below TLR4 activation thresholds discussed in the literature. Elevated endotoxin has been associated with fibroblast activation, neuroinflammatory gene-expression changes, and epithelial barrier disruption in primary cell models.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across 4 sequential passes
Sterility & mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma is controlled by 0.1 µm mycoplasma-retentive filtration at every production stage (not tested per lot by USP <63>).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEMH-QPR1X Minimum Essential Medium (MEM), Low Glucose, NEAA and 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid ? Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish ? Microfluidics Suitable MEM Low Glucose plus NEAA plus 25mM HEPES for organ-on-a-chip applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-QPR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-QPR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic handling systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM reduces particulate-clogging risk in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline and phenol-red-free formulation support confocal, TEER-sensor, and biosensor workflows on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] L-Glutamine, [-] Sodium Pyruvate, [-] Phenol Red
Appearance Pale Yellow-colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 290–330 mOsm/kg H2O
Total ingredients 35
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 recommended (sodium bicarbonate + 25 mM HEPES dual buffering)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 35 ingredients verified per lot with CAS numbers, across 3 tabbed views (Inorganic Salts; Amino Acids; Vitamins & Others). HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) and i-Inositol are listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
i-Inositol 87-89-8 2.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-QPR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016–certified quality management system. Final QA at the DiagnoCine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water, minimizing trace-metal and organic-carbon contribution to every lot.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration count: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290–330 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMH-QPR1X compares

FluxMPS™ DCP-MEMH-QPR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH-QPR1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Not classified Not classified
MEM + NEAA + 25mM HEPES — no L-Glutamine, no Pyruvate, no Phenol Red; imaging-clean complete metabolic control check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-QPR1X.

Yes. DCP-MEMH-QPR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering an ultra-low particulate baseline for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with mycoplasma-retentive filtration purity is particularly suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.
 
No phenol red is included, supporting autofluorescence-conscious imaging workflows; no glutamine and no pyruvate are included, leaving nitrogen and carbon inputs fully researcher-defined. HEPES (25 mM) works alongside sodium bicarbonate for stable pH. This configuration suits confocal or TEER-monitored primary cell OoC work where metabolic inputs must be independently controlled and supplemented per protocol.
5% CO2 is recommended. This formulation carries sodium bicarbonate (2200 mg/L) plus 25 mM HEPES as a dual buffering system; the bicarbonate component is calibrated to hold physiological pH at approximately 5% CO2, while HEPES adds stability during brief periods outside the incubator.
Yes. L-Glutamine, sodium pyruvate, phenol red, serum, and growth factors can all be added per your protocol. For serum or other protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — a 0.04 µm membrane is not appropriate for serum, as it retains immunoglobulins, lipoproteins, and other serum macromolecules. Defined, protein-free additions may use a 0.1 µm membrane.
Endotoxin is controlled per manufacturing batch to a release specification of < 0.05 EU/mL, verified by LAL assay per USP <85> before release. A low, verified specification is relevant for primary cell work, where elevated endotoxin has been associated in the literature with TLR4-mediated changes in fibroblast, neuronal, and epithelial cell behavior.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid

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