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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMH-QBR1X
$49.50
DCP-MEMH-QBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-MEMH-QBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for primary fibroblasts, primary neurons, epithelial and vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final polish targets sub-mycoplasma particulate levels well below conventional 0.22 µm–filtered media. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides CO2-independent pH buffering for open-air handling. Formulation: [+] Earle's Salts (Calcium, Magnesium), [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Pyruvate (110 mg/L), [+] 25 mM HEPES (5958 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red.

  • Low Glucose (1.0 g/L) — a physiological carbon source for primary cells sensitive to high-glucose osmotic stress.
  • NEAA pre-loaded (Gly, Ala, Asn, Asp, Glu, Pro) — reduces de novo synthesis burden and ammonia accumulation in primary cell culture.
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO2-independent pH stability for atmospheric incubation, open-well handling, and flow cytometry prep.
  • Bicarbonate-free and phenol-red-free formulation — a defined base for optical, biosensor, and CO2-independent perfusion applications.
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) — a repeated prefilter + final-filter pair run twice for full redundancy.
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch.
  • Manufactured under an ISO 13485:2016 quality management system with micro-batch, per-lot QC release.
  • Custom pH, glucose, HEPES concentration and component modifications available on request.
DCP-MEMH-QBR1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Sodium Pyruvate, [+] 25mM HEPES
    [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
  • AppearancePale yellow, clear solution (phenol red?free)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235–275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a foundational primary-cell medium, but conventional 0.22 µm–filtered MEM passes particulates and endotoxin that can alter primary cell phenotype. FluxMPS™ reduces these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 particulate compliance. Low-particulate media helps prevent chip microchannel clogging in sub-100 µm geometries.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA is a long-established base for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: CO2-stable pH

25 mM HEPES limits pH drift during open-air suspension handling, flow cytometry prep, and multi-well assay setup performed outside a CO2 incubator.

shield

Low endotoxin release specification

< 0.05 EU/mL release specification (batch-tested, USP <85>). Lower endotoxin loads reduce the risk of LPS-driven fibroblast activation and epithelial barrier disruption in primary cell assays.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, and HEPES concentration modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would pass through.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-MEMH-QBR1X runs a two-pair, four-pass filtration train (0.1 µm ×2 + 0.04 µm ×2) versus the single 0.22 µm pass used in conventional MEM.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot) at every production stage; mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEMH-QBR1X Minimum Essential Medium (MEM) Low Glucose NEAA and 25mM HEPES without L-Glutamine Sodium Bicarbonate Phenol Red 1X Liquid, Quadruple-stage filtration system 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II polish, Microfluidics Suitable MEM Low Glucose plus NEAA plus 25mM HEPES for organ-on-a-chip and microphysiological system applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-QBR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-QBR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity supporting microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic liquid-handling systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm Microfluidics Suitable cut-off.
  • Valve & Sensor Protection: Helps reduce micro-fouling risk in delicate chip geometries.
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture.

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle's Salts + NEAA + low glucose is a physiologically relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle's salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps reduce particulate clogging risk in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Phenol-red-free formulation with ultra-low particulate levels supports confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts (Calcium, Magnesium), [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Pyruvate (110 mg/L), [+] 25 mM HEPES (5958 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow, clear solution (phenol red?free)
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present (Gly, Ala, Asn, Asp, Glu, Pro)
pH USP <791> 7.4
Osmolality USP <785> 235–275 mOsm/kg H2O
Total ingredients 35 (across 4 composition categories)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 35 ingredients across 4 composition categories, released per lot with CAS numbers where known. NEAA components are listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Sodium Pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-QBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured by ISO 13485-certified suppliers. Final QA at the DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm feed water, controlled for trace metals and organic carbon (TOC).

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — a Certificate of Analysis is available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), by light obscuration.

Osmolality — USP <785>

Target: 235–275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMH-QBR1X compares

FluxMPS™ DCP-MEMH-QBR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH-QBR1X (FluxMPS™) Conventional MEM Low Glucose (0.22 µm filtered) Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
HEPES-buffered, bicarbonate-free & phenol-red-free with NEAA check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-QBR1X.

Yes. DCP-MEMH-QBR1X is processed through a quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, giving it Microfluidics Suitable status for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.
FluxMPS™ runs four sequential filtration passes as two prefilter + final-filter pairs: 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish). Conventional media use a single 0.22 µm pass. The 0.1 µm stages are mycoplasma-retentive by pore size (not tested per lot); the 0.04 µm stages provide additional sub-mycoplasma margin.
This formulation is HEPES-buffered for CO2-independent handling and phenol-red-free to avoid autofluorescence and absorbance interference in imaging and biosensor assays. L-Glutamine is left out so researchers can add it fresh (commonly 2–4 mM, or a stabilized dipeptide substitute) at a concentration matched to their cell type, since glutamine degrades in liquid storage. Sodium pyruvate (110 mg/L) is already included as an alternative energy substrate.
No. This formulation is bicarbonate-free and CO2-independent: 25 mM HEPES alone maintains pH 7.2–7.4 at 37°C without gas supplementation, which is useful for atmospheric incubation, open-well handling, and flow cytometry preparation.
Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; a 0.04 µm membrane is too fine for serum and will retain lipoproteins and strip serum activity. Defined, protein-free additions can use a 0.1 µm filter. Contact support@diagnocine.com for supplementation guidance specific to your cell type.
Each manufacturing batch is released to a specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before shipment. Endotoxin is controlled per batch, not per unit. For primary cells, keeping endotoxin low helps avoid TLR4-mediated fibroblast activation, neuroinflammatory gene expression, and epithelial barrier disruption.
Yes. A full CoA is available per lot, covering appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell and OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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