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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid

Product#: DCP-MEMH-QR1X
$49.50
DCP-MEMH-QR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Phenol Red

FluxMPS™ DCP-MEMH-QR1X is a Microfluidics Suitable, ultra-filtered MEM Low Glucose + NEAA + 25mM HEPES formulation engineered for primary fibroblasts and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides robust pH buffering for open-air handling. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] L-Glutamine, [-] Phenol Red.

  • Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high glucose
  • NEAA pre-loaded — reduces metabolic burden and ammonia accumulation from de novo synthesis
  • 25 mM HEPES (pKa 7.3 at 37°C) — pH stability for open-air handling, flow cytometry prep, and atmospheric incubation
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish; endotoxin < 0.05 EU/mL
  • Formulated without L-Glutamine and without Phenol Red — fresh nitrogen control and an autofluorescence-free imaging base
  • Manufactured under an ISO 13485:2016 quality management system with Ultrapure Type 1 water (18.2 MΩ·cm) and ISO Class 5 aseptic fill
DCP-MEMH-QR1X | Sizes: 500 mL, 1000 mL | Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA + 25mM HEPES
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES
    [-] L-Glutamine, [-] Phenol Red
  • AppearancePale yellow, clear solution (phenol red–free)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290–330 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes particulates and endotoxin that can alter primary cell phenotype. FluxMPS™ reduces these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate primary cell media helps prevent chip channel clogging.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial, and vascular cells.

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HEPES: pH stability

25 mM HEPES helps prevent pH rise during open-air suspension culture handling, flow cytometry prep, and multi-well assay setup outside CO2 incubators.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin release specification — below the TLR4 activation threshold, relevant for LPS-sensitive fibroblast, neuronal, and epithelial models.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

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Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains particles ≥ 0.04 µm, including mycoplasma (0.2–0.3 µm) — a size class that passes standard 0.22 µm filtration unchanged.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

FluxMPS™ DCP-MEMH-QR1X runs a paired prefilter + final-filter train, twice, reaching a 0.04 µm final pore size with 0.04 µm mycoplasma-retentive filtration validated at every production stage (not tested per lot).

4
Sterile filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma (0.2–0.3 µm) controlled via 0.04 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEMH-QR1X Minimum Essential Medium (MEM) Low Glucose NEAA 25mM HEPES w/o L-Glutamine Phenol Red 1X Liquid ? Quadruple-stage filtration system 0.1 μm x2 + 0.04 μm x2 for organ-on-a-chip OoC and microfluidic primary cell culture applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMH-QR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMH-QR1X is purpose-built for primary fibroblasts and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotics platforms.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate, phenol red–free formulation supports confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] L-Glutamine, [-] Phenol Red
Appearance Pale yellow, clear solution (phenol red–free)
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 290–330 mOsm/kg H2O
Total ingredients 36
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 recommended (dual HEPES + bicarbonate buffering; HEPES alone maintains pH without CO2)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA + 25mM HEPES: 36 ingredients verified per lot with CAS numbers. NEAA is listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMH-QR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm feed water, controlling trace metals and organic carbon.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every batch.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), by light obscuration.

Osmolality — USP <785>

Target: 290–330 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMH-QR1X compares

FluxMPS™ DCP-MEMH-QR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEMH-QR1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
MEM + NEAA + 25mM HEPES — no L-Glutamine, no Phenol Red check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.04 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes cancel Risk of clogging cancel Risk of clogging

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMH-QR1X.

Yes. DCP-MEMH-QR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, giving low particulate levels suited to MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with 0.04 µm mycoplasma-retentive filtration purity is particularly suited to primary neuronal, epithelial, and fibroblast OoC platforms.
FluxMPS™ uses four sequential filtration passes, run as two dedicated prefilter + final-filter pairs — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II — reaching a 0.04 µm final pore size with 0.04 µm mycoplasma-retentive filtration validated at every production stage (not tested per lot), versus a single 0.22 µm pass for conventional media.
Phenol Red is removed for an autofluorescence-free imaging base; L-Glutamine is removed for fresh, lab-controlled supplementation at time of use since it degrades in liquid storage. HEPES (25 mM) provides pH stability during open-air handling and confocal sessions. This is a preferred base for live-cell imaging of primary neuronal or epithelial OoC platforms requiring precise nitrogen control.
5% CO2 is recommended. The formulation is dual-buffered with sodium bicarbonate and 25 mM HEPES; HEPES alone can maintain pH during brief periods without CO2, such as open-bench handling or imaging.
Yes. L-Glutamine, serum, and growth factors can be added fresh at time of use. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm membrane for serum, as it will strip essential serum proteins and clog. Defined, protein-free additions may use a 0.1 µm membrane.
Every manufacturing batch is tested by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet a release specification of < 0.05 EU/mL before release. This is a batch-level specification, not a per-unit certificate. For primary cells, endotoxin activates TLR4, which can alter fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.
Yes. A full CoA is available per batch, covering appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA + 25mM HEPES in primary cell and OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
  10. Sart S, et al. Metabolic profiling of primary cells cultured in defined media. Trends Biotechnol. 2017;35:113–125. doi:10.1016/j.tibtech.2016.08.006

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