0
Precision Quality to Your Research verified ISO 13485 Certified | SBA WOSB | NJ MWBE · NYC MWBE · Illinois BEP arrow_forward View Details

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMG-QPBRN1X
$34.10
DCP-MEMG-QPBRN1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains Calcium Contains Magnesium Contains Glucose Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red Without Sodium Pyruvate

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. Processed through a validated four-stage filtration train (0.1 µm ×2 + 0.04 µm ×2), it is built to be microchannel-safe from day one.

  • High-glucose (4.5 g/L) MEM base with Non-Essential Amino Acids (NEAA) — supports high-energy-demand cell types
  • Formulated without L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, and Phenol Red for fully customizable, defined supplementation
  • Quadruple-stage nano-filtration: 0.1 µm ×2 (Prefiltration I & II) + 0.04 µm ×2 (Final filtration I & II — Polish)
  • Endotoxin release specification: < 0.05 EU/mL (USP <85> BET), tested per manufacturing batch
  • pH 7.4 (USP <791>); Osmolality per Certificate of Analysis (USP <785>)
  • Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under ISO Class 5 aseptic fill & finish
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMG-QPBRN1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L)
  • L-GlutamineNot added — supplement as needed
  • Sodium PyruvateNot added — supplement as needed
  • HEPESNot added
  • NEAAIncluded
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See Certificate of Analysis
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO

Available sizes: 500 mL, 1000 mL. Contact support@diagnocine.com for pricing and availability.

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized organisms (0.2–0.3 µm), subvisible debris, and lot-to-lot endotoxin variation that accumulate inside microchannels — corrupting biosensor readings, stressing primary cultures, and shortening device lifetimes. FluxMPS™ is engineered to reduce these failure modes at the source.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance is verified per lot.

target

High-glucose metabolic support

4.5 g/L glucose supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm) meeting ASTM D1193 / ISO 3696 ultrapure water standards, supporting low trace-metal and organic-carbon background.

visibility

Low background for imaging

Low particulate baseline supports confocal microscopy, live-cell biosensors, and TEER measurements without the light scatter typical of 0.22 µm-filtered media.

science

Rich, stable nutrient profile

NEAA-supplemented formulation plus micro-batch precision manufacturing ensures comprehensive amino acid coverage for demanding cell culture models.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid is processed through a four-stage serial filtration train reaching a 0.04 µm final cut-off, engineered to retain mycoplasma-sized organisms and subvisible particulates that a single 0.22 µm pass does not address.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish performed in a validated ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

Four sequential stages reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every production lot.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every batch undergoes 14-day USP <71> sterility testing and must show no growth to pass release. Mycoplasma control is achieved through 0.1 µm / 0.04 µm dual-pair filtration (not tested per lot per USP <63>).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Minimum Essential Medium (MEM) High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red 1X Liquid (DCP-MEMG-QPBRN1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering sub-mycoplasma purity for MPS and OoC applications.
© Diagnocine® — DCP-MEMG-QPBRN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid is validated for organ-on-a-chip, cancer biology, metabolic research, live-cell imaging, and primary/stem cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 0.01 µm (10 nm) filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the MPS Grade 0.01 µm variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation supports laminar flow integrity across complex chip geometries, reducing microchannel clogging risk.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High-glucose base supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultrapure, low-endotoxin formulation supports sensitive iPSC differentiation protocols where endotoxin variation causes off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurement in perfusion models.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

High-glucose, bicarbonate-free, phenol-red-free base enables 13C isotope tracing, glycolytic flux studies, and Agilent Seahorse XF real-time metabolic assays.

13C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate baseline supports high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
Appearance Pale-yellow colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> See Certificate of Analysis
Glucose 4500 mg/L (4.5 g/L)
L-Glutamine Not added — supplement as needed
Sodium Pyruvate Not added — supplement as needed
Phenol Red Not added
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement No bicarbonate buffering system; CO2 incubation optional — supplement with HEPES for non-CO2 culture
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 4500.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water for low trace-metal and organic-carbon background.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in a validated ISO Class 5 laminar-flow workstation; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL, controlled per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>; result reported on the Certificate of Analysis.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com.
Product Comparison

How DCP-MEMG-QPBRN1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives.

Parameter DCP-MEMG-QPBRN1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm) Standard MEM High Glucose alternative
Grade Microfluidics Suitable Standard grade Standard grade
Base formulation MEM High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red MEM High Glucose Standard MEM High Glucose Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL		 Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEMG-QPBRN1X is processed through our Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2), delivering ultra-low particulate counts that help prevent microchannel clogging in OoC and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or many subvisible particulates that can accumulate in microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1.
These components are intentionally left out so you can control your own culture conditions: add L-Glutamine or a stable dipeptide substitute (e.g. GlutaMAX) at your standard working concentration, add sodium pyruvate if your protocol calls for it, add sodium bicarbonate if you plan to buffer with CO2, and add phenol red only if visual pH indication is required for your assay. None of these four components are present in the base formulation, so none will interfere with your supplementation.
This formulation contains no sodium bicarbonate, so CO2 incubation is optional rather than required. For non-CO2 culture, supplement with HEPES per your protocol. If you add sodium bicarbonate for CO2-buffered culture, incubate under the CO2 level appropriate to the bicarbonate concentration you use.
Yes. This medium can be supplemented with FBS (typically 5–20%), growth factors, antibiotics, or other additives per standard practice. If you sterile-filter a serum or protein-containing supplement before adding it, use a 0.2 µm low-protein-binding PES or PVDF filter — never 0.04 µm, which retains much of the protein and lipoprotein fraction serum depends on. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before shipment. Lot-specific results are documented on the Certificate of Analysis, available from support@diagnocine.com.
Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), lot number, manufacturing date, expiry, raw material traceability, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  8. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

Related Products

Recently Viewed

Satisfaction
Quality Rating
Value Rating
Style Rating

X