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FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-MEMG-QPRN1X
$34.10
DCP-MEMG-QPRN1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Phenol Red Without HEPES

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • High glucose (4.5 g/L, 4500 mg/L) formulation with Non-Essential Amino Acids (NEAA) — supports high-energy-demand cell types
  • Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, engineered for microchannel-scale flow geometries
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> Bacterial Endotoxins Test)
  • pH 7.4 (USP <791>); formulated without L-glutamine, without phenol red, and without HEPES — sodium pyruvate present at 110 mg/L
  • Bicarbonate-buffered (2200 mg/L NaHCO3); requires an approximately 5.8% CO2 atmosphere to maintain pH 7.4 (validate per cell line/incubator)
  • Prepared with Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 aseptic fill & finish
  • Manufactured under an ISO 13485:2016 quality management system; final packaging, testing, and customization at Diagnocine Precision, Totowa, NJ
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMG-QPRN1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L)
  • L-GlutamineNot added — supplement as needed
  • Sodium Pyruvate110 mg/L
  • HEPESNot added
  • NEAAIncluded
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variation that accumulate inside microchannels — corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ addresses these failure modes with a validated four-pass filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particulates to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per batch.

target

High-glucose metabolic support

4.5 g/L glucose supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm), meeting ASTM D1193 / ISO 3696 Type I purity standards, with trace-metal and organic-carbon (TOC) control.

visibility

Low background for imaging

Ultra-low particulate load supports high-content confocal microscopy and biosensor integration. Riboflavin (0.100 mg/L) is a native component and contributes autofluorescence to account for in fluorescence-based assay design.

science

Rich, stable nutrient profile

NEAA-supplemented formulation plus micro-batch precision ensures comprehensive amino acid coverage for demanding cell culture models.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is processed through a validated four-pass filtration train — a repeated prefilter-and-final-filter pair run twice in series — reaching a 0.04 µm final cut-off. This addresses mycoplasma-sized particulates, subvisible debris, and bioburden that single-pass 0.22 µm filtration does not remove.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates, including mycoplasma-sized organisms (0.2–0.3 µm), that pass through a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge and providing full train redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish performed in a validated ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

The four-pass train reaching a 0.04 µm final cut-off delivers approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 (light obscuration) compliance verified on every production lot.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is mitigated through the 0.1 µm mycoplasma-retentive stages of the filtration train (not tested per lot). No bacterial or fungal growth observed.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Minimum Essential Medium (MEM) High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red 1X Liquid (DCP-MEMG-QPRN1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering sub-mycoplasma purity for MPS and OoC applications.
© Diagnocine® — DCP-MEMG-QPRN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is validated for organ-on-a-chip, cancer biology, stem cell, vascular biology, metabolomics, and live-cell imaging applications where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion, adding 0.02 µm and 0.01 µm stages after this product's 0.04 µm polish.

  • Total Particulate Exclusion: 0.01 µm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation prevents microchannel clogging and maintains laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High-glucose base supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin variation causes off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurement in perfusion models.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

High-glucose base enables 13C isotope tracing and glycolytic flux experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium; this formulation contains 2200 mg/L sodium bicarbonate.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load supports high-content confocal imaging and biosensor integration; riboflavin (0.100 mg/L) contributes native autofluorescence to account for in fluorescence-based assay design.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Contains sodium bicarbonate, calcium, magnesium, glucose, and sodium pyruvate; without L-glutamine, phenol red, and HEPES
Appearance Pale-yellow colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 4500 mg/L (4.5 g/L)
L-Glutamine Not added
Sodium Pyruvate 110 mg/L
Phenol Red Not added
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696)
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Approximately 5.8% CO2 (derived from 2200 mg/L NaHCO3 at pH 7.4; validate per cell line/incubator)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 4500.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water meeting ASTM D1193 / ISO 3696 Type I purity standards.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing ensures lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay performed on every manufacturing batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per batch.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: See CoA.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com with your lot number.
Product Comparison

How DCP-MEMG-QPRN1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives.

Parameter DCP-MEMG-QPRN1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm) Standard MEM High Glucose alternative
Grade Microfluidics Suitable Not specified Not specified
Formulation Contains sodium bicarbonate, calcium, magnesium, glucose, and sodium pyruvate; without L-glutamine, phenol red, and HEPES MEM High Glucose Standard MEM High Glucose Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)		 Varies by supplier
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEMG-QPRN1X uses our Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm), delivering ultra-low particulate counts that prevent microchannel clogging in OoC and MPS devices. It is Microfluidics Suitable at a 0.04 µm final cut-off.
Standard 0.22 µm filtration leaves intact mycoplasma-sized organisms (0.2–0.3 µm) and subvisible particulates that accumulate in microchannels. FluxMPS™ uses four sequential passes reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1 (light obscuration).
The 4.5 g/L (4500 mg/L) glucose level supports metabolically demanding cell types including HeLa, MCF-7, fibroblasts, and high-density suspension cultures. L-glutamine is not added to this formulation and should be supplemented at time of use (or via a stabilized dipeptide form) per your protocol. Sodium pyruvate (110 mg/L) is already included. Contact support@diagnocine.com for a custom formulation.
This formulation contains 2200 mg/L sodium bicarbonate (no HEPES) and requires an approximately 5.8% CO2 atmosphere to maintain physiological pH 7.4. Validate the exact CO2 setpoint for your specific incubator and cell line.
Yes. This formulation can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. Filter-sterilize serum-containing supplements using a 0.2 µm low-protein-binding PES or PVDF membrane before addition — do not use a 0.04 µm membrane for supplement filtration, as it will strip essential serum proteins and lipoproteins. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before shipment. Batch-specific results are documented in the Certificate of Analysis, available from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  8. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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