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FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Product#: DCP-MEMG-QBN1X
$34.10
DCP-MEMG-QBN1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • High-glucose (4.5 g/L) Eagle’s Minimum Essential Medium formulated without L-glutamine and without sodium bicarbonate, giving full user control over buffering and glutamine supplementation
  • Quadruple-stage filtration train: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II — Polish), reaching a 0.04 µm final cut-off for microchannel-safe purity
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), controlled per manufacturing batch
  • Contains phenol red (11 mg/L) as a pH indicator; appearance is an orange-to-red, clear solution
  • Sodium pyruvate (110 mg/L) included as a supplemental carbon/energy source
  • Manufactured under an ISO 13485:2016 quality management system, with final packaging, testing, and customization at Diagnocine, Totowa, NJ
  • pH 7.4 (USP <791>); osmolality reported per Certificate of Analysis
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMG-QBN1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L)
  • L-GlutamineNot added — supplement as needed
  • Sodium BicarbonateNot added
  • Sodium Pyruvate110 mg/L
  • Phenol Red11 mg/L (present)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is built to address these failure modes at the filtration stage.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 particulate compliance verified per lot.

target

High-glucose metabolic support

4.5 g/L glucose supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm) for low trace-metal and organic-carbon (TOC) content, reducing lot-to-lot chemical variability.

visibility

Low background for imaging

Ultra-low particulate load supports confocal microscopy and live-cell biosensor work. This formulation contains phenol red; for assays requiring zero optical background, a phenol-red-free custom formulation is available on request.

science

Rich, stable nutrient profile

Standard Eagle’s essential amino acid profile plus micro-batch precision ensures comprehensive coverage for demanding cell culture models.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is processed through a four-stage filtration sequence reaching a 0.04 µm final cut-off, addressing mycoplasma-scale particulates and subvisible debris that 0.22 µm filtration does not retain.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter allows through.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge in the redundant pairing.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill performed in an ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

Four sequential stages reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every production lot.

5×
 
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every production batch undergoes 14-day USP <71> sterility testing. Mycoplasma risk is controlled via 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Minimum Essential Medium (MEM) High Glucose w/o L-Glutamine, Sodium Bicarbonate 1X Liquid (DCP-MEMG-QBN1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering sub-mycoplasma purity for MPS and OoC applications.
© Diagnocine® — DCP-MEMG-QBN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is validated for organ-on-a-chip, cancer biology, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion. This is a distinct grade tier from the Microfluidics Suitable product on this page — see the Grade note above.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation reduces microchannel clogging risk and supports laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High-glucose base supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered formulation supports sensitive iPSC differentiation protocols where endotoxin variability can cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurements in perfusion models.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

High-glucose base supports 13C isotope tracing and glycolytic flux experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium — this formulation contains phenol red.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load supports confocal microscopy and biosensor integration. This formulation contains phenol red, which may interfere with certain fluorescence and absorbance channels — a phenol-red-free custom formulation is available on request.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation MEM High Glucose (4.5 g/L) with sodium pyruvate and phenol red; without L-glutamine and without sodium bicarbonate
Appearance Orange-to-red colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 4500 mg/L (4.5 g/L)
L-Glutamine Not added — supplement as needed
Sodium Pyruvate 110 mg/L
Phenol Red 11 mg/L (present)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement No sodium bicarbonate and no HEPES included; add sodium bicarbonate for standard CO2 incubation, or add HEPES (10–25 mM) for CO2-independent culture
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Pack sizes 500 mL, 1000 mL
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Total: 28 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others). Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 11.000
Sodium Pyruvate 113-24-6 110.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity Type 1 water, controlled for trace metals and organic carbon (TOC).

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay on every manufacturing batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result reported in the Certificate of Analysis.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com.
Product Comparison

How DCP-MEMG-QBN1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives.

Parameter DCP-MEMG-QBN1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm) Standard MEM High Glucose alternative
Grade Microfluidics Suitable Not specified Not specified
Base Formulation MEM High Glucose (4.5 g/L) with sodium pyruvate and phenol red; without L-glutamine and without sodium bicarbonate MEM High Glucose Standard MEM High Glucose Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEMG-QBN1X uses our quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), delivering ultra-low particulate counts that help prevent microchannel clogging in OoC and MPS devices. It is Microfluidics Suitable at a 0.04 µm final cut-off.
Standard 0.22 µm filtration leaves intact mycoplasma-sized particulates (0.2–0.3 µm) and subvisible debris that can accumulate in microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1.
L-Glutamine and sodium bicarbonate are omitted so labs can control buffering strategy and glutamine stability independently — glutamine degrades in liquid storage, so many labs prefer to add it fresh at time of use, and bicarbonate level determines the CO2 concentration required for pH control. Add L-glutamine (2 mM typical) and sodium bicarbonate per your protocol, or add HEPES for CO2-independent culture. This formulation already contains 4.5 g/L glucose, 110 mg/L sodium pyruvate, and phenol red — no additional supplementation of those three is needed. Contact support@diagnocine.com for a custom formulation.
This formulation contains no sodium bicarbonate and no HEPES. For standard CO2 incubator culture, supplement with sodium bicarbonate per your protocol; alternatively, add HEPES (10–25 mM) for CO2-independent culture.
Yes. This medium can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. Add supplements immediately before use. Serum and other protein-containing additions should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm filter for supplements, as it will strip serum of the proteins and lipoproteins that make it functional.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> BET before release, with a release specification of < 0.05 EU/mL. Batch-specific results are documented in the Certificate of Analysis, available from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, lot number, expiry, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  8. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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