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FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- High-glucose (4.5 g/L) Minimum Essential Medium supplemented with Non-Essential Amino Acids (NEAA) — suited to high-energy-demand cell types
- Quadruple-stage filtration train: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II — Polish)
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), controlled per manufacturing batch
- Formulated without sodium bicarbonate and without sodium pyruvate — confirm buffering and carbon-source strategy before use
- pH 7.4 (USP <791>); osmolality reported on the Certificate of Analysis (CoA)
- Contains phenol red (11 mg/L) as a visual pH indicator
- Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
- Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
- Glucose4500 mg/L (4.5 g/L)
- L-Glutamine292 mg/L
- NEAAIncluded (7 non-essential amino acids)
- Sodium PyruvateNot added
- Sodium BicarbonateNot added
- pH (USP <791>)7.4
- Osmolality (USP <785>)See CoA
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm-filtered media carry mycoplasma-sized organisms (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings, stressing primary cultures, and shortening device lifetimes. FluxMPS™ addresses these failure modes with a validated four-stage filtration train.
Microchannel-safe purity
0.04 µm final filter reduces sub-visible particulate load well below 0.22 µm media; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.
High-glucose metabolic support
4.5 g/L glucose plus a full NEAA supplement supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.
Ultrapure-grade water
Prepared with Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon control, reducing feed-water contribution to formulation variability.
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy, live-cell biosensors, and TEER measurements. Note: this formulation contains phenol red, which carries inherent absorbance/fluorescence — a phenol-red-free variant is available on request for optical assays requiring a colorless base.
Rich, stable nutrient profile
NEAA-supplemented MEM formulation plus micro-batch precision manufacturing ensures comprehensive amino acid coverage for demanding cell culture models.
Customization on demand
pH, glucose, salts, HEPES, sodium bicarbonate, and full nutrient composition available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is processed through a four-pass, paired prefilter/final-filter sequence reaching a 0.04 µm final cut-off — a validated improvement over the subvisible-particle and mycoplasma-sized-organism carryover typical of single-pass 0.22 µm filtration.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final-filter cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated 0.1 µm prefilter, protecting the second 0.04 µm cartridge and providing filtration-train redundancy.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish performed in a validated ISO Class 5 laminar-flow workstation.
Performance vs. conventional media
Four sequential passes reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared with single-pass 0.22 µm filtration.
© Diagnocine® — DCP-MEMG-PB1X
Designed for next-generation cell culture platforms
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is validated for organ-on-a-chip, cancer biology, stem cell, vascular, and imaging applications where particulate contamination and endotoxin variability are unacceptable.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion beyond the 0.04 µm Microfluidics Suitable tier described above.
- Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
- Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
- Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs
Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
Ultra-filtered formulation supports laminar flow integrity across complex microchannel geometries without particulate-driven blockage.
Warburg Effect & Metabolic Research
High-glucose base supports Warburg-effect studies and glucose uptake assays in cancer cell lines.
iPSC-Derived Models
Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin variability causes off-target effects.
Endothelial & Primary Cells
Microchannel-safe purity supports endothelial barrier integrity and TEER measurement in perfusion models.
Metabolic Flux Analysis
High-glucose base enables precise 13C isotope tracing and glycolytic flux experiments.
Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate load supports confocal imaging and optical biosensor integration; a phenol-red-free variant is available for TEER and fluorescence assays sensitive to background absorbance.
Lot-release quality parameters
Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid undergoes the quality-release battery below before shipment.
| Parameter | Specification |
|---|---|
| Formulation | Contains: L-Glutamine, Phenol Red, Calcium, Magnesium, Glucose. Without: Sodium Bicarbonate, HEPES, Sodium Pyruvate. |
| Appearance | Orange-to-red colored, clear solution (phenol red present) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | See CoA |
| Glucose | 4500 mg/L (4.5 g/L) |
| L-Glutamine | 292 mg/L |
| Sodium Pyruvate | Not added |
| Phenol Red | 11 mg/L (present) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | Compliant |
| Particulate ≥25 µm USP <788> Method 1 | Compliant |
| Water Purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. ISO 13485 | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Not recommended |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Not required (bicarbonate-free formulation) |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot documentation, CoA available |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 · Molecular biology and cell culture growth media · (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch precision manufacturing |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
Complete formulation with CAS numbers, reproduced from manufacturer specification. Total: 35 components across 4 categories. Custom compositions available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.900 |
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Asparagine monohydrate | 15.000 | |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30189-89-0 | 31.300 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 51.900 | |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
| i-Inositol | 87-89-8 | 2.000 |
ISO 13485:2016 manufacturing & compliance
Manufactured under an ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering manufacturing, testing, and release for every production lot.
Ultrapure Type 1 Water
All media prepared with 18.2 MΩ·cm resistivity Type 1 water for trace-metal and organic-carbon control.
ISO Class 5 Fill & Finish
Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.
Micro-Batch Precision
Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.
Osmolality — USP <785>
Freezing-point osmometry per USP <785>. Result: See CoA.
Documentation — CoA & Full Lot Records
Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-MEMG-PB1X (FluxMPS™) compares
Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives.
| Parameter | DCP-MEMG-PB1X (FluxMPS™) | Conventional MEM High Glucose (0.22 µm) | Standard MEM High Glucose alternative |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard grade (0.22 µm) | Standard grade (0.22 µm) |
| Base Formulation | MEM, High Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid | MEM High Glucose Standard | MEM High Glucose Equivalent |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 stages | 1 stage | 1–2 stages |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Variable | Variable |
| Microfluidic channel compatibility | check_circle Validated | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle On request | cancel | Limited |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.
Supporting literature
Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.
- Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
- Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
- Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
- Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
- Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
- Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
- Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
- Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
- Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
