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FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-MEMG-QPR1X
$44.00
DCP-MEMG-QPR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains Calcium Contains Magnesium Contains Glucose Without L-Glutamine Without Phenol Red Without HEPES Without Sodium Pyruvate

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. The quadruple-stage train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration — keeping microchannels clear of sub-mycoplasma particulates from first use.

  • High-glucose (4.5 g/L) MEM formulation with Non-Essential Amino Acids (NEAA) — supports high-energy-demand cell types
  • Formulated without L-Glutamine, Sodium Pyruvate, and Phenol Red — supplement fresh at time of use per your protocol
  • Quadruple-stage filtration train: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II — Polish), reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
  • pH 7.4 (USP <791>); osmolality reported per Certificate of Analysis (USP <785>)
  • Prepared with Ultrapure Type 1 water; aseptic fill under an ISO 13485:2016 quality management system
  • Manufactured and released at Diagnocine, Totowa, NJ, with full lot traceability and CoA available on request
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMG-QPR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L)
  • L-GlutamineNot added — supplement as needed
  • Sodium PyruvateNot added — supplement as needed
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See Certificate of Analysis
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protected from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack, insulated container
Available pack sizes: 500 mL, 1000 mL.
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and batch-to-batch endotoxin variation that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ addresses these failure modes with a validated quadruple-stage filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.

target

High-glucose metabolic support

4.5 g/L glucose supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm) meeting ASTM D1193 / ISO 3696 standards for trace-metal and organic-carbon (TOC) control.

visibility

Low background for imaging

Low particulate baseline from quadruple-stage filtration supports confocal microscopy, live-cell biosensors, and TEER measurements without particulate interference.

science

Rich, stable nutrient profile

Nineteen amino acids plus NEAA supplementation and micro-batch precision ensure comprehensive nutrient coverage for demanding cell culture models.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is processed through a four-stage serial filtration sequence reaching a 0.04 µm final cut-off — addressing mycoplasma-sized and subvisible particulates that 0.22 µm filtration cannot retain.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) not captured by 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm cartridge, giving the train full redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish performed in an ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration, with USP <788> Method 1 compliance verified on every production lot.

5×
Finer final pore size than conventional 0.22 µm media (pore-size ratio)
4
Sequential filtration passes: 0.1 µm ×2 + 0.04 µm ×2
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter. No bacterial or fungal growth observed in sterility testing.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Minimum Essential Medium (MEM) High Glucose NEAA w/o L-Glutamine Sodium Pyruvate Phenol Red 1X Liquid (DCP-MEMG-QPR1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish for organ-on-a-chip, tissue-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off for Microfluidics Suitable cell culture media.
© Diagnocine® — DCP-MEMG-QPR1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is validated for organ-on-a-chip, cancer biology, stem cell, vascular, metabolomics, and live-cell imaging applications where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the finest particulate exclusion beyond Microfluidics Suitable specifications.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: reduces particulate-induced blockage risk in precision fluidic systems
  • Extended Perfusion Stability: supports flow-rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation supports laminar flow integrity across microchannel geometries in chip-based culture systems.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High-glucose (4.5 g/L) base supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin variation causes off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER-based assays in perfusion models.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

High-glucose base enables precise ¹³C isotope tracing and glycolytic flux experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load from quadruple-stage filtration supports high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation High Glucose (4.5 g/L) MEM + NEAA; contains Sodium Bicarbonate, Calcium, Magnesium, Glucose; without L-Glutamine, Phenol Red, HEPES, Sodium Pyruvate
Appearance Pale yellow to colorless, clear solution (phenol red-free)
pH USP <791> 7.4
Osmolality USP <785> See Certificate of Analysis
Glucose 4500 mg/L (4.5 g/L)
L-Glutamine Not added — supplement as needed
Sodium Pyruvate Not added — supplement as needed
Phenol Red Not added
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (release specification — see §Manufacturing & Compliance)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water purity Ultrapure Type 1 water (18.2 MΩ·cm), ASTM D1193 / ISO 3696
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100) aseptic fill
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement Approximately 5% CO₂ (derived from 2200 mg/L sodium bicarbonate buffering to maintain pH 7.4)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Total: 34 components across 4 formulation categories (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 browsable tabs below. Reproduced from manufacturer specification with CAS numbers where available. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate   15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30189-89-0 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate   51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 4500.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system with final packaging, testing, and customization at Diagnocine in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water meeting ASTM D1193 / ISO 3696 standards.

biotech

ISO Class 5 Fill & Finish

Aseptic filling performed in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay performed on every batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result reported on the Certificate of Analysis.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Certificate of Analysis: Request via support@diagnocine.com with your lot number.
Product Comparison

How DCP-MEMG-QPR1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives, and against published supplier endotoxin specifications.

Parameter DCP-MEMG-QPR1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm) Standard MEM High Glucose alternative
Grade Microfluidics Suitable Standard grade (0.22 µm) Standard grade (0.22 µm)
Base Formulation MEM High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red MEM High Glucose Standard MEM High Glucose Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as “Not specified”.

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEMG-QPR1X uses our quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), reaching a 0.04 µm final cut-off that helps keep microchannels clear of sub-mycoplasma particulates in OoC, ToC, and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm). FluxMPS™ uses four sequential stages (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, five times finer than 0.22 µm, with USP <788> Method 1 particulate compliance verified per lot.
These three components are left out so labs can control their own glutamine source (L-glutamine or a stabilized dipeptide such as GlutaMAX), pyruvate concentration, and keep the medium colorless for optical assays. Typical supplementation is 2–4 mM L-glutamine and 1 mM sodium pyruvate, added fresh at time of use; phenol red can be added back separately if visual pH indication is desired, though pH is best confirmed with a calibrated meter or the Certificate of Analysis. Contact support@diagnocine.com for guidance specific to your cell type.
Yes. This formulation contains sodium bicarbonate (2200 mg/L) as its primary buffer, which requires an approximately 5% CO₂ atmosphere to maintain physiological pH (7.4).
Yes. FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid can be supplemented with FBS (5–20%), growth factors, antibiotics, L-glutamine, and sodium pyruvate per standard practice. For serum or protein-containing additions, filter through a 0.2 µm low-protein-binding PES or PVDF membrane immediately before use — a 0.04 µm membrane is not recommended for supplement filtration, as it retains IgM and lipoprotein fractions and can strip serum of its activity. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before shipment. Lot-specific results are documented in the Certificate of Analysis, available from support@diagnocine.com.
Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  8. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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