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FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

Product#: DCP-MEMG-QP1X
$44.00
DCP-MEMG-QP1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without HEPES

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) high-glucose MEM engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • High-glucose (4.5 g/L) MEM formulation with Non-Essential Amino Acids (NEAA); L-glutamine and HEPES are not included — supplement per your protocol
  • Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, four passes, reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), controlled per manufacturing batch
  • Sodium pyruvate included at 110 mg/L to support supplemental carbon metabolism
  • Sodium bicarbonate buffered (2200 mg/L); approximately 5–6% CO₂ atmosphere recommended for this formulation
  • Manufactured under an ISO 13485:2016 quality management system with lot-specific Certificate of Analysis
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMG-QP1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L)
  • L-GlutamineNot added — supplement as needed
  • Sodium Pyruvate110 mg/L
  • HEPESNot added
  • NEAAIncluded
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and lot-to-lot endotoxin variation that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ addresses these failure modes through a validated four-stage filtration process.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 particulate compliance verified per lot.

target

High-glucose metabolic support

4.5 g/L glucose plus 110 mg/L sodium pyruvate supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm) to ASTM D1193 / ISO 3696 standards, controlling trace-metal and organic-carbon content.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy and biosensor integration. Note: this formulation contains phenol red (see Composition), which should be considered for fluorescence and absorbance assays.

science

Rich, stable nutrient profile

NEAA-supplemented formulation with per-lot release testing ensures comprehensive amino acid coverage for demanding cell culture models.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid is processed through a four-stage filtration sequence — two paired 0.1 µm prefilter / 0.04 µm final-filter cycles — reaching a 0.04 µm final cut-off. Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter; the second pair is not polishing the first pair's output, it is protecting the second 0.04 µm cartridge.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including mycoplasma-sized organisms (0.2–0.3 µm diameter).

  3. 3

    0.1 µm Prefiltration II

    Second, dedicated prefilter protecting the second 0.04 µm cartridge; provides full redundancy for the final polish.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish performed in a validated ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

Four sequential stages reaching a 0.04 µm final cut-off are reported to deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every production lot.

4
Filtration passes — 0.1 µm ×2 + 0.04 µm ×2
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing with no bacterial or fungal growth observed. Mycoplasma risk is controlled through 0.1 µm mycoplasma-retentive filtration; lots are not individually tested for mycoplasma by USP <63>.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid (DCP-MEMG-QP1X) Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering a 0.04 µm final cut-off for Microfluidics Suitable applications.
© Diagnocine® — DCP-MEMG-QP1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid is suited to organ-on-a-chip, cancer biology, stem cell, vascular, metabolomics, and live-cell imaging models where particulate contamination and endotoxin variability are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion beyond the Microfluidics Suitable tier described above.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Microfluidics Suitable formulation reduces microchannel clogging risk and helps maintain laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High-glucose base with sodium pyruvate supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered formulation supports sensitive iPSC differentiation protocols where endotoxin variability causes off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurement in perfusion models.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

High-glucose, pyruvate-containing base supports ¹³C isotope tracing and glycolytic flux experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load supports high-content confocal imaging and optical biosensor integration; contains phenol red (see Composition).

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation High-glucose MEM with NEAA; contains Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, Glucose, Sodium Pyruvate; without L-Glutamine and HEPES
Appearance Orange-to-red colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 4500 mg/L (4.5 g/L)
L-Glutamine Not added — supplement as needed
Sodium Pyruvate 110 mg/L
Phenol Red 11 mg/L (present)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release spec)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696)
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement Yes, approximately 5–6% CO₂ (sodium bicarbonate-buffered, 2200 mg/L)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation reproduced from the manufacturing specification, with CAS registry numbers added where available. This formulation totals 36 components across 4 categories: Inorganic Salts, Amino Acids, Vitamins, and Others. Every lot is manufactured and released against this profile; custom compositions are available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate   15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30189-89-0 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate   51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 11.000
Sodium Pyruvate 113-24-6 110.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water (ASTM D1193 / ISO 3696 Type I), controlling trace-metal and organic-carbon content.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay per manufacturing batch. Release specification: < 0.05 EU/mL. Assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result reported on the Certificate of Analysis.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com with your lot number.
Product Comparison

How DCP-MEMG-QP1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives.

Parameter DCP-MEMG-QP1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm) Standard MEM High Glucose alternative
Grade Microfluidics Suitable Not applicable (0.22 µm filtered) Not applicable (0.22 µm filtered)
Base Formulation MEM, High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate MEM High Glucose Standard MEM High Glucose Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEMG-QP1X is Microfluidics Suitable, processed through our Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), delivering a 0.04 µm final cut-off that reduces the particulate load associated with microchannel clogging in OoC and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or many subvisible particulates that can accumulate in microchannels. FluxMPS™ uses four sequential stages reaching a 0.04 µm final cut-off, reported to deliver approximately 5× lower particulate counts per USP <788> Method 1.
L-Glutamine is omitted because it degrades in liquid storage; add L-glutamine or a stable dipeptide substitute (e.g., GlutaMAX) to a final concentration appropriate for your cell line at time of use. HEPES is also not included; add 10–25 mM HEPES if additional pH buffering is required for extended open-air handling. Contact support@diagnocine.com for a custom pre-supplemented formulation.
Yes. This formulation is sodium bicarbonate-buffered (2200 mg/L) and requires an atmosphere of approximately 5–6% CO₂ to maintain a physiological pH of 7.4.
Yes. FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Pyruvate: 1X Liquid can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before use; do not use a 0.04 µm membrane for serum, as it will retain lipoproteins and clog rapidly. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> BET (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL. Batch-specific results are documented in the CoA, available from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  8. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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