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FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMG-QBR1X
$44.00
DCP-MEMG-QBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red Without HEPES

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • High-glucose (4.5 g/L) Minimum Essential Medium (MEM) with Non-Essential Amino Acids (NEAA) for energy-demanding cell types
  • Formulated without L-Glutamine, Sodium Bicarbonate, or Phenol Red — add L-Glutamine and buffer per your protocol
  • Quadruple-stage ultra-filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> Bacterial Endotoxins Test)
  • Sodium pyruvate (110 mg/L) included as an alternative carbon/energy source
  • Manufactured under an ISO 13485:2016 quality management system with aseptic fill & finish
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMG-QBR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L)
  • L-GlutamineNot added — supplement per protocol
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protected from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is built to a tighter filtration standard from the outset.

filter_alt

Microchannel-safe purity

0.04 µm final filter limits particulate load; USP <788> Method 1 (light obscuration) particulate testing targeted per lot.

target

High-glucose metabolic support

4.5 g/L glucose supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696); low trace-metal and organic-carbon (TOC) content supports sensitive downstream assays.

visibility

Low background for imaging

Ultra-low particulate baseline from quadruple-stage filtration supports confocal microscopy, live-cell biosensors, and TEER measurements.

science

Rich, stable nutrient profile

NEAA-supplemented formulation plus micro-batch precision ensures comprehensive amino acid coverage for demanding cell culture models.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid is processed through a four-pass filtration train — two dedicated prefilter + final-filter pairs — reaching a 0.04 µm final pore size, well beyond what single-pass 0.22 µm filtration achieves.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge downstream.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm cartridge, giving the train full redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish performed in a validated ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

Four sequential passes reaching a 0.04 µm final cut-off deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration.

4
Filtration passes: 0.1 µm ×2 + 0.04 µm ×2
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is controlled via 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter. No bacterial or fungal growth observed in sterility testing.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid (DCP-MEMG-QBR1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II - Polish, for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering sub-mycoplasma purity for MPS and OoC applications.
© Diagnocine® — DCP-MEMG-QBR1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid is validated for organ-on-a-chip, cancer biology, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion — a separate tier from the 0.04 µm Microfluidics Suitable formulation described on this page.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation prevents microchannel clogging and maintains laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High-glucose base supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin variability causes off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurements in perfusion models.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

High-glucose, bicarbonate-free base is compatible with 13C isotope tracing, NMR metabolomics, and Agilent Seahorse XF assays.

13C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load ideal for high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Contains Calcium, Magnesium, Glucose, Sodium Pyruvate; without L-Glutamine, Sodium Bicarbonate, Phenol Red, HEPES
Appearance Colorless to pale straw, clear solution (phenol red-free)
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 4500 mg/L (4.5 g/L)
L-Glutamine Not added — supplement per protocol
Sodium Pyruvate 110 mg/L
Phenol Red Not added
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (release specification)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696)
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf Life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Optional (no sodium bicarbonate); supplement with HEPES for non-CO2 culture conditions
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Total: 34 components, organized under 4 formulation categories (Inorganic Salts, Amino Acids, Vitamins, Others) across the 3 tabs below, reproduced from manufacturer specification with CAS numbers where known. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate   15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30189-89-0 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate   51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
Sodium Pyruvate 113-24-6 110.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system, with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

Media prepared with 18.2 MΩ·cm resistivity water, minimizing trace-metal and organic-carbon contribution.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing ensures lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

Release specification: < 0.05 EU/mL. Verified by LAL assay on every batch.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: See CoA.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com with your lot number.
Product Comparison

How DCP-MEMG-QBR1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives and published supplier specifications.

Parameter DCP-MEMG-QBR1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm) Standard MEM High Glucose alternative
Grade Microfluidics Suitable Not designated Not designated
Base Formulation MEM, High Glucose, NEAA, without L-Glutamine, Sodium Bicarbonate, Phenol Red MEM High Glucose Standard MEM High Glucose Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 passes 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as “Not specified”.

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEMG-QBR1X uses our Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), delivering ultra-low particulate counts that help prevent microchannel clogging in OoC and MPS devices. It is a Microfluidics Suitable product, filtered to a 0.04 µm final cut-off.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or many subvisible particulates. FluxMPS™ uses four sequential passes — two 0.1 µm prefilter + 0.04 µm final-filter pairs — reaching a 0.04 µm final cut-off, delivering approximately 5× lower particulate counts.
L-Glutamine is omitted because it degrades over time in liquid storage; add it fresh (typically 2–4 mM, or as GlutaMAX) immediately before use. Sodium Bicarbonate and Phenol Red are omitted to give you control over buffering strategy and to keep the medium free of a pH indicator dye for optical assays. Sodium pyruvate (110 mg/L) is already included as a supplemental carbon/energy source. Contact support@diagnocine.com for a custom pre-supplemented formulation.
This formulation does not contain sodium bicarbonate. CO2 requirement is therefore reduced; supplement with HEPES for non-CO2 (ambient) culture, or maintain standard CO2 incubation with your own buffering strategy. Validate against your specific cell line.
Yes. This formulation can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. Serum and protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane immediately before addition — never a 0.04 µm membrane, which will strip serum of essential high-molecular-weight components (IgM, lipoproteins) and clog rapidly. Add supplements immediately before use.
Every production lot is controlled to a release specification of < 0.05 EU/mL by LAL assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch, and results are documented in the Certificate of Analysis, available from support@diagnocine.com.
Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, lot number, expiry, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  8. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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