Does DCP-MEMG-QB1X require a CO2 incubator?

This formulation does not contain sodium bicarbonate, so CO2 incubation is optional. For extended culture without a CO2 incubator, supplement with HEPES (10-25 mM).

FluxMPS Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Q: What makes the filtration process superior to standard 0.22 micron filtered media?

Standard 0.22 micron filtration does not retain mycoplasma-sized organisms (0.2-0.3 micron) or subvisible particulates that accumulate in microchannels. FluxMPS uses two paired prefilter and final-filter stages reaching 0.04 micron, delivering approximately 5x lower particulate counts per USP less than 788.

Q: Can DCP-MEMG-QB1X be supplemented with serum, growth factors, or other components?

Yes. Supplement with FBS (5-20%), growth factors, antibiotics, or other additives per standard practice. Add immediately before use, and filter serum-containing or protein-containing additions through a 0.2 micron low-protein-binding PES or PVDF filter, never 0.04 micron.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. A lot-specific CoA is available for every shipment and includes appearance, pH, osmolality, endotoxin, sterility, particulate matter, raw material traceability, lot number, expiry, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.

ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Phenol Red Calcium Magnesium Glucose Sodium Pyruvate L-Glutamine Sodium Bicarbonate HEPES

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, Quadruple-stage ultra-filtered cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

High-glucose (4.5 g/L) MEM formulation with Non-Essential Amino Acids (NEAA) — supports high-energy-demand cell types
Quadruple-stage filtration train: 0.1 µm Prefiltration I & II paired with 0.04 µm Final filtration I & II (0.1 µm ×2 + 0.04 µm ×2)
Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), verified per manufacturing batch
Formulated without L-glutamine, sodium bicarbonate, or HEPES — a bicarbonate-free base ready for user-defined buffering
pH 7.4 (USP <791>); phenol red included for visual pH monitoring
Manufactured under an ISO 13485:2016 quality management system, with final QC and packaging at Diagnocine, Totowa, NJ
Sodium pyruvate (110 mg/L) included as an alternative carbon/energy substrate
Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request

DCP-MEMG-QB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Glucose4500 mg/L (4.5 g/L)
L-GlutamineNot added — supplement as needed
Sodium Pyruvate110 mg/L
HEPESNot added
NEAAIncluded
pH (USP <791>)7.4
Osmolality (USP <785>)See Certificate of Analysis
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and lot-to-lot particulate variation that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ addresses these failure modes with a validated four-stage filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.

target

High-glucose metabolic support

4.5 g/L glucose supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696), minimizing trace-metal and organic-carbon (TOC) background.

visibility

Low background for imaging

Reduced particulate baseline from Quadruple-stage filtration supports confocal microscopy, live-cell biosensors, and TEER measurements. Note: this formulation contains phenol red and riboflavin — a phenol-red-free custom formulation is available for autofluorescence-sensitive assays.

science

Rich, stable nutrient profile

NEAA-supplemented formulation plus micro-batch precision ensures comprehensive amino acid coverage for demanding cell culture models.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is processed through a four-stage filtration train — two dedicated prefilter + final-filter pairs — reaching a 0.04 µm final pore size, substantially reducing mycoplasma-sized and subvisible particulates relative to single-pass 0.22 µm filtration.

1

0.1 µm Prefiltration I

Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a 0.22 µm filter.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge; not a polish of Stage 2 effluent.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; aseptic fill and finish performed under validated ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

Four sequential stages reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration.

5×

0.04

µm final filter pore size — sub-mycoplasma polishing, 4 total passes

Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is mitigated via 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are approximately 0.2–0.3 µm in diameter.

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate 1X Liquid (DCP-MEMG-QB1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I and II, 0.04 micron Final filtration I and II, for organ-on-a-chip and microfluidic applications by Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering sub-mycoplasma purity for MPS and OoC applications.
© Diagnocine® — DCP-MEMG-QB1X

Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is validated for organ-on-a-chip, cancer biology, stem cell, vascular, metabolic, and live-cell imaging models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Quadruple-stage filtered formulation prevents microchannel clogging and maintains laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS

Cancer Biology

Warburg Effect & Metabolic Research

High-glucose base supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080

Stem Cell Biology

iPSC-Derived Models

Ultra-filtered formulation supports sensitive iPSC differentiation protocols where particulate load and endotoxin cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity essential for maintaining endothelial barrier integrity and TEER values in perfusion models.

HUVECsBHK-21Primary fibroblasts

Metabolomics

Metabolic Flux Analysis

High-glucose base enables precise ¹³C isotope tracing and glycolytic flux experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium (this formulation contains phenol red).

¹³C tracingNMR metabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Reduced particulate load from Quadruple-stage filtration supports high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER

Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid undergoes the quality-release battery below before shipment.

Physical & Chemical Parameters

Parameter	Specification
Formulation	NEAA, Phenol Red, Calcium, Magnesium, Glucose (4.5 g/L), Sodium Pyruvate; without L-Glutamine, Sodium Bicarbonate, HEPES
Appearance	Orange-to-red colored, clear solution (phenol red present)
pH USP <791>	7.4
Osmolality USP <785>	See Certificate of Analysis
Glucose	4500 mg/L (4.5 g/L)
L-Glutamine	Not added — supplement as needed
Sodium Pyruvate	110 mg/L
Phenol Red	11 mg/L (present)

Sterility, Purity & Safety Parameters

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL (batch release specification — see § Manufacturing & Compliance)
Sterility USP <71>	No growth after 14 days
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	Compliant
Particulate ≥25 µm USP <788> Method 1	Compliant
Water Purity	Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696)
Manufacturing std. ISO 13485	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, protected from light
Freeze-thaw	Not recommended
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	Bicarbonate-free; CO₂ supplementation optional. For extended non-CO₂ culture, supplement with HEPES (10–25 mM)
Available pack sizes	500 mL, 1000 mL

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Cell culture / reagent grade
Traceability	Full lot documentation, CoA available
Manufacturing QMS	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch precision manufacturing
Intended use	For Research Use Only (RUO)

Formulation

Full composition (mg/L)

Full formulation of 35 components, organized here into three navigable tabs reflecting the original INORGANIC SALTS, AMINO ACIDS, VITAMINS, and OTHERS category headings. Custom compositions available on request.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	265.000
Magnesium sulfate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	400.000
Sodium chloride	7647-14-5	6800.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	122.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	7.500
L-Alanine	56-41-7	8.900
L-Arginine hydrochloride	1119-34-2	126.000
L-Asparagine monohydrate	5794-13-8	15.000
L-Aspartic acid	56-84-8	13.300
L-Cystine dihydrochloride	30189-89-0	31.300
L-Glutamic acid	56-86-0	14.700
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	52.000
L-Leucine	61-90-5	52.000
L-Lysine hydrochloride	657-27-2	72.500
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	32.000
L-Proline	147-85-3	11.500
L-Serine	56-45-1	10.500
L-Threonine	72-19-5	48.000
L-Tryptophan	73-22-3	10.000
L-Tyrosine disodium salt dihydrate		51.900
L-Valine	72-18-4	46.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	1.000
D-Ca-Pantothenate	137-08-6	1.000
Folic acid	59-30-3	1.000
Nicotinamide	98-92-0	1.000
Pyridoxal hydrochloride	65-22-5	1.000
Riboflavin	83-88-5	0.100
Thiamine hydrochloride	67-03-8	1.000
OTHERS
i-Inositol	87-89-8	2.000
D-Glucose	50-99-7	4500.000
Phenol red sodium salt	34487-61-1	11.000
Sodium pyruvate	113-24-6	110.000

Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.

Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water (ASTM D1193 / ISO 3696), minimizing trace-metal and organic-carbon background.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay per batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result documented in the Certificate of Analysis.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request via support@diagnocine.com.

Product Comparison

How DCP-MEMG-QB1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives, and against published supplier endotoxin specifications.

Parameter	DCP-MEMG-QB1X (FluxMPS™)	Conventional MEM High Glucose (0.22 µm)	Standard MEM High Glucose alternative
Grade	Microfluidics Suitable	Not specified	Not specified
Base Formulation	MEM, High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid	MEM High Glucose Standard	MEM High Glucose Equivalent
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 stages	1 stage	1–2 stages
Mycoplasma barrier filtration	check_circle	cancel	cancel
Endotoxin (release specification)	FluxMPS™ — < 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance	check_circle USP <788> Method 1	cancel	cancel
Water quality	Ultrapure Type 1 (18.2 MΩ·cm)	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	Variable	Variable
Microfluidic channel compatibility	check_circle Validated	cancel Risk of clogging	cancel Risk of clogging
Custom formulation	check_circle On request	cancel	Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Is DCP-MEMG-QB1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-MEMG-QB1X uses our Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2), delivering low particulate counts that help prevent microchannel clogging in OoC and MPS devices. It is Microfluidics Suitable at a 0.04 µm final cut-off.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or subvisible particulates that accumulate in microchannels. FluxMPS™ uses two paired prefilter + final-filter stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788>.

Why are L-glutamine and sodium bicarbonate excluded, and how do I supplement for my cell type?expand_more

This formulation ships without L-glutamine and without sodium bicarbonate so the buffering and glutamine source can be set at time of use. Add L-glutamine (or a stable dipeptide substitute) to your target concentration, and add sodium bicarbonate or HEPES depending on your incubation environment. Contact support@diagnocine.com for a custom pre-formulated option.

Does DCP-MEMG-QB1X require a CO₂ incubator?expand_more

This formulation does not contain sodium bicarbonate, so CO₂ incubation is optional. For extended culture without a CO₂ incubator, supplement with HEPES (10–25 mM).

Can DCP-MEMG-QB1X be supplemented with serum, growth factors, or other components?expand_more

Yes. FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. Add supplements immediately before use, and filter serum-containing or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter (never 0.04 µm, which retains IgM, lipoproteins, and much of serum's functional protein fraction).

What endotoxin level should I expect, and how is it verified?expand_more

Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay per USP <85> Bacterial Endotoxins Test and must meet the release specification of < 0.05 EU/mL (assay sensitivity 0.005 EU/mL) before shipment. Lot-specific results are documented in the Certificate of Analysis, available from support@diagnocine.com.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, lot number, expiry, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.

Scientific References

Supporting literature

Peer-reviewed publications supporting ultra-filtered MEM formulations and microfluidic cell culture applications.

Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Engineered where standard media fails

Microchannel-safe purity

High-glucose metabolic support

Ultrapure-grade water

Low background for imaging

Rich, stable nutrient profile

Customization on demand

Quadruple-stage filtration system

0.1 µm Prefiltration I

0.04 µm Final filtration I

0.1 µm Prefiltration II

0.04 µm Final filtration II — Polish

Performance vs. conventional media

Designed for next-generation cell culture platforms

Automated Bioreactors & Robotics

Micro Physiological System (MPS) & Chip

Warburg Effect & Metabolic Research

iPSC-Derived Models

Endothelial & Primary Cells

Metabolic Flux Analysis

Microscopy & Optical Sensing

Lot-release quality parameters

Full composition (mg/L)

ISO 13485:2016 manufacturing & compliance

ISO 13485:2016 QMS

Ultrapure Type 1 Water

ISO Class 5 Fill & Finish

Micro-Batch Precision

Endotoxin — USP <85> BET

Particulate — USP <788> Method 1

Osmolality — USP <785>

Documentation — CoA & Full Lot Records

How DCP-MEMG-QB1X (FluxMPS™) compares

Frequently asked questions

Supporting literature

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