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FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-MEMGH-PR1X
$49.50
DCP-MEMGH-PR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-MEMGH-PR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM High Glucose + NEAA + 25 mM HEPES formulation engineered for primary neurons and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides robust pH buffering outside a CO2 incubator. Formulation: [+] Earle's Salts, [+] NEAA, [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25 mM HEPES | [-] Sodium Pyruvate, [-] Phenol Red.

  • High Glucose (4.5 g/L) with Earle's Salts and NEAA supports energy-demanding primary neurons, cardiomyocytes and epithelial cells.
  • NEAA pre-loaded reduces de novo synthesis burden and ammonia accumulation in low-serum primary cell culture.
  • 25 mM HEPES (pKa 7.3 at 37°C) buffers pH during open-air handling, flow cytometry preparation and atmospheric incubation.
  • Formulated without sodium pyruvate and without phenol red for imaging-clean, low optical-interference primary cell assays.
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — Microfluidics Suitable for OoC and MPS platforms.
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), verified per manufacturing batch.
  • Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis.
DCP-MEMGH-PR1X Sizes: 500 mL, 1000 mL Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
  • Media familyMEM High Glucose + NEAA + 25mM HEPES (Earle's Salts base)
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Phenol Red, [-] Sodium Pyruvate
  • AppearancePale yellow, clear solution (phenol red–free)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290–330 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a foundational primary cell medium, but conventional 0.22 µm filtered MEM permits mycoplasma, subvisible particulates and endotoxin that can alter primary cell phenotype. FluxMPS™ addresses these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> particulate compliance. Low-particulate primary cell media helps prevent chip channel clogging in sub-100 µm geometries.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial and vascular cells.

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HEPES: pH stability outside CO2

25 mM HEPES helps prevent pH rise during open-air suspension handling, flow cytometry prep, and multi-well assay setup outside a CO2 incubator.

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Low endotoxin release specification

< 0.05 EU/mL endotoxin release specification — below the threshold conventionally associated with TLR4 activation, relevant to preventing confounding fibroblast activation, neuroinflammatory signaling, and epithelial barrier disruption in primary cell assays.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

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Customization on demand

pH, glucose, HEPES concentration and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions. The train is a repeated prefilter-plus-final-filter pair run twice, not a single descending cascade.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates, including the 0.2–0.3 µm size range associated with mycoplasma.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter under ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across 4 filtration passes
Sterility: No growth after 14-day incubation (USP <71>). Mycoplasma: Controlled via 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEMGH-PR1X Minimum Essential Medium (MEM), High Glucose, NEAA and 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM for organ-on-a-chip (OoC) and microphysiological system (MPS) applications | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMGH-PR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMGH-PR1X is formulated for primary neurons and related primary cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic liquid handling systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm cut-off.
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries.
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture.

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle's Salts + NEAA provides a physiologically balanced base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle's salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate levels and a phenol red–free formulation reduce optical interference for confocal imaging, TEER sensors and biosensor applications on primary cell chips. Note: this formulation retains riboflavin, which itself contributes background fluorescence at its own excitation/emission range.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every batch released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Phenol Red, [-] Sodium Pyruvate
Media family MEM High Glucose + NEAA + 25mM HEPES (Earle's Salts base)
Appearance Pale yellow, clear solution (phenol red–free)
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present (Gly, Ala, Asn, Asp, Glu, Pro, Ser)
pH USP <791> 7.4
Osmolality USP <785> 290–330 mOsm/kg H2O
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 recommended; sodium bicarbonate (2200 mg/L) provides primary buffering, with 25 mM HEPES supplementing pH stability outside the incubator
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM High Glucose + NEAA + 25mM HEPES: 36 ingredients across 4 categories, verified per batch with CAS numbers. NEAA components are listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMGH-PR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

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ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016–certified quality management system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water supports low trace-metal and organic carbon (TOC) content in the feedwater.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested; a Certificate of Analysis is issued for every batch.

Endotoxin — USP <85> BET

LAL assay; batch release specification < 0.05 EU/mL; assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290–330 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMGH-PR1X compares

FluxMPS™ DCP-MEMGH-PR1X vs. conventional 0.22 µm–filtered MEM formulations and published supplier specifications.

Parameter DCP-MEMGH-PR1X (FluxMPS™) Conventional 0.22 µm–filtered media / published specifications
Grade Microfluidics Suitable Not specified
MEM + Earle's Salts + NEAA + 25 mM HEPES, without sodium pyruvate and without phenol red check_circle Yes cancel Uncommon combination
Final filtration pore size 0.04 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stage) cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested (Method 1) check_circle Yes cancel Not specified
Water quality Ultrapure Type 1, 18.2 MΩ·cm Purified water (grade often unspecified)
Manufacturing QMS ISO 13485:2016 ISO 9001 or unspecified
Microfluidic channel compatibility check_circle Microfluidics Suitable (0.04 µm filtered) cancel Risk of channel clogging
Custom formulation available check_circle Yes cancel Typically no

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMGH-PR1X.

Yes. Processed through a quadruple-stage filtration system reaching a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2), DCP-MEMGH-PR1X is Microfluidics Suitable for OoC, ToC, LoC and MPS platforms. The MEM High Glucose + NEAA + 25 mM HEPES formulation with 0.1 µm mycoplasma-retentive filtration supports primary neuronal, epithelial, fibroblast and vascular chip models.
FluxMPS™ uses four sequential filtration passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off versus the single 0.22 µm pass used in conventional media. This provides additional mycoplasma-retentive filtration and lower particulate levels for microfluidic channel compatibility.
This MEM formulation is provided without sodium pyruvate and without phenol red to give researchers control over pyruvate-dependent metabolic assays and to minimize optical interference. If your cell type requires an alternative carbon source or redox buffer, sodium pyruvate can be added at the bench; defined, protein-free stock solutions may be filtered at 0.1 µm before addition. Contact support@diagnocine.com for a custom formulation with sodium pyruvate pre-added.
5% CO2 is recommended; sodium bicarbonate (2200 mg/L) provides the primary buffering system, and the included 25 mM HEPES (pKa 7.3 at 37°C) supplements pH stability during open-air handling outside the incubator.
Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; a 0.04 µm membrane is not recommended for serum, as it retains immunoglobulins, lipoproteins and other components below its cut-off. Defined, protein-free additions may be filtered at 0.1 µm.
Each batch is released to a specification of less than 0.05 EU/mL, tested by LAL assay per USP <85> (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before release. Endotoxin is controlled per manufacturing batch rather than per unit.
Yes. A CoA is available for every batch and covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM High Glucose + NEAA + 25mM HEPES in primary cell and OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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