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FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMGH-BR1X
$49.50
DCP-MEMGH-BR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-MEMGH-BR1X is a Microfluidics Suitable, ultra-filtered MEM High Glucose + NEAA + 25mM HEPES formulation engineered for primary neurons and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers ultra-low particulate levels versus conventional 0.22 µm–filtered media. NEAA is pre-loaded, reducing de novo synthesis burden on primary cells. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO2-independent pH buffering in a bicarbonate-free, phenol red-free base.

  • High Glucose (4.5 g/L, 4500 mg/L) supports energy-demanding primary cell types including neurons and cardiomyocytes
  • NEAA pre-loaded — reduces metabolic burden and ammonia accumulation from de novo amino acid synthesis
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO2-independent pH buffering; suited to open-air handling, flow cytometry prep, and atmospheric incubation
  • Bicarbonate-free and phenol red-free formulation — reduces background autofluorescence for imaging and biosensor workflows
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • 36 formulation components verified per lot, including 20 amino acids and 8 vitamins at standard MEM concentrations
DCP-MEMGH-BR1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Media familyMEM High Glucose + NEAA + 25mM HEPES
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] Earle's Salts, [+] NEAA, [+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] Sodium Bicarbonate, [-] Phenol Red
  • AppearancePale yellow-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235 - 275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage / Shelf Life2-8°C, protect from light / 12 months from manufacture
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a primary cell biology standard — but conventional 0.22 µm filtered MEM passes mycoplasma-sized particles, subvisible particulates, and higher endotoxin than sensitive primary cells and OoC microchannels tolerate. FluxMPS™ addresses these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 particulate compliance helps prevent chip channel clogging in sub-100 µm geometries.

biotech

Primary cell-optimized formulation

MEM + Earle's Salts + NEAA is a long-established base for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

HEPES: CO2-stable pH

25 mM HEPES helps limit pH drift during open-air suspension handling, flow cytometry prep, and multi-well assay setup outside CO2 incubators.

shield

Low endotoxin release specification

< 0.05 EU/mL release specification, tested per batch by LAL assay (USP <85>) — relevant where endotoxin exposure can influence primary fibroblast, neuronal, and epithelial cell behavior.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis demand on primary cells, which may improve viability in low-serum conditions and reduce ammonia accumulation.

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Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates that pass a standard 0.22 µm filter, including material in the mycoplasma size range.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-MEMGH-BR1X is processed through a four-pass, paired-cartridge filtration train reaching a 0.04 µm final cut-off, versus the single 0.22 µm pass typical of conventional MEM.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final pore size
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms typically measure 0.2-0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEMGH-BR1X Minimum Essential Medium (MEM) High Glucose NEAA and 25mM HEPES without Sodium Bicarbonate Phenol Red 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II - Microfluidics Suitable MEM High Glucose plus NEAA plus 25mM HEPES for organ-on-a-chip applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMGH-BR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMGH-BR1X is formulated for primary neurons and related primary cell models, with 0.04 µm filtration purity supporting microfluidic platform use.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic handling systems (see the Grade note in the Purity Architecture section above).

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates the 0.04 µm tier does not target
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle's Salts + NEAA is a physiologically established base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

MEM with NEAA supports primary epithelial cells and barrier-integrity assays in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle's salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate, phenol red-free formulation reduces background autofluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every batch released against the full specification matrix. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow-colored, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
Sodium Pyruvate 110 mg/L
pH USP <791> 7.4
Osmolality USP <785> 235 - 275 mOsm/kg H2O
Total ingredients 36
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — bicarbonate-free, 25 mM HEPES-buffered formulation; validate pH stability for your specific application
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM High Glucose + NEAA + 25mM HEPES: 36 ingredients verified per lot with CAS numbers, grouped by formulation category (Inorganic Salts, Amino Acids, Vitamins, Others).

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
HEPES 7365-45-9 5958.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMGH-BR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water with tight trace-metal and organic-carbon control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every batch.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 235 - 275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Certificate of Analysis: Request for any DCP-MEMGH-BR1X batch at support@diagnocine.com.
Product Comparison

How DCP-MEMGH-BR1X compares

FluxMPS™ DCP-MEMGH-BR1X vs. conventional 0.22 µm-filtered MEM High Glucose + NEAA formulations, and published supplier endotoxin specifications.

Parameter DCP-MEMGH-BR1X (FluxMPS™) Comparison
Grade Microfluidics Suitable (0.04 µm final filtration) Standard filtration grade (0.22 µm, uncontrolled)
Bicarbonate-free, phenol red-free, HEPES-buffered MEM check_circle Yes Conventional MEM typically includes phenol red and/or sodium bicarbonate
Final filtration pore size 0.04 µm 0.22 µm (conventional MEM/DMEM)
Number of filtration stages 4 (Quadruple-stage: 0.1 µm ×2 + 0.04 µm ×2) 1 (single-stage, 0.22 µm)
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stages) cancel No
HEPES (25 mM) included check_circle Yes Usually not included in standard MEM
NEAA included check_circle Yes Optional add-on in most standard MEM formulations
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate testing check_circle Yes Not typically tested
Water quality Ultrapure Type 1, 18.2 MΩ·cm Purified water (typical)
Manufacturing QMS ISO 13485:2016 ISO 9001 or none (typical)
Microfluidic channel compatibility check_circle Yes cancel Risk of channel clogging (0.22 µm particulate carryover)

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMGH-BR1X.

Yes. DCP-MEMGH-BR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size. MEM High Glucose + NEAA + 25mM HEPES, bicarbonate-free and phenol red-free, with an endotoxin release specification < 0.05 EU/mL, is suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.
FluxMPS™ runs four sequential passes as two dedicated prefilter + final-filter pairs: 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II. This reaches a 0.04 µm final cut-off versus the single 0.22 µm pass typical of conventional media, with mycoplasma-retentive filtration (0.1 µm) built into the train.
Sodium bicarbonate is excluded because pH is buffered by 25 mM HEPES, allowing use outside a CO2 incubator. Phenol red is excluded to reduce background autofluorescence for imaging and biosensor applications. NEAA is pre-loaded (Glycine, L-Alanine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Proline, and related non-essential amino acids), reducing de novo synthesis demand on primary cells. If your application requires bicarbonate/CO2 buffering or a phenol red indicator, contact support@diagnocine.com for the corresponding formulation variant.
No. This formulation is bicarbonate-free and buffered with 25 mM HEPES (pKa 7.3 at 37°C), which is designed for CO2-independent use. Validate pH stability under your specific vessel, seal, and incubation conditions before extended culture.
Yes. When adding serum or other protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane to preserve serum components such as growth factors and lipoproteins. Defined, protein-free additions may be filtered at 0.1 µm. Do not use a 0.04 µm membrane for supplement filtration — it retains serum proteins and lipoproteins and clogs rapidly.
The release specification is < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification. A Certificate of Analysis is available on request.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM High Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432-437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117-129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j

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