Is a Certificate of Analysis available, and what does it contain?

Yes. A full CoA per lot covers appearance, pH, osmolality, sterility, endotoxin, mycoplasma filtration status, particulate count (USP less than 788 Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.

FluxMPS Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Q: Does this medium require a CO2 incubator?

Approximately 5 percent CO2 is recommended. The formulation contains both sodium bicarbonate and 25 mM HEPES: bicarbonate buffering benefits from a CO2 incubator, while HEPES alone can maintain pH during short periods of open-air handling outside CO2.

Q: Can this medium be supplemented with serum, growth factors, or other components?

Yes. Serum and other protein-containing supplements should be filtered through a 0.2 um low-protein-binding PES or PVDF membrane before addition, never through a 0.04 um membrane, which retains serum proteins and lipoproteins. Defined, protein-free additions may use a 0.1 um filter.

Q: What endotoxin level should I expect, and how is it verified?

DCP-MEMGH-QPR1X is produced to a release specification of less than 0.05 EU/mL by LAL assay (USP less than 85), controlled per manufacturing batch. Every batch is tested before release. For primary cells, endotoxin activates TLR4 signaling, which can influence fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.

verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Sodium Bicarbonate 25mM HEPES Calcium Magnesium High Glucose (4500 mg/L) L-Glutamine Phenol Red Sodium Pyruvate

FluxMPS™ DCP-MEMGH-QPR1X is a Microfluidics Suitable, ultra-filtered MEM High Glucose + NEAA + 25mM HEPES formulation engineered for primary neurons and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides robust pH buffering during open-air handling.

High Glucose (4.5 g/L) supports energy-demanding primary cell types such as neurons and cardiomyocytes.
NEAA pre-loaded — reduces de novo synthesis burden and ammonia accumulation in primary cultures.
25 mM HEPES (pKa 7.3 at 37°C) provides pH stability during open-air handling, flow cytometry prep, and atmospheric incubation.
Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaches a final 0.04 µm polish for microfluidic channel safety.
Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) — relevant to TLR4-sensitive primary cultures.
Formulated without L-glutamine, sodium pyruvate, or phenol red for full researcher-defined metabolic and optical control.
Manufactured under an ISO 13485:2016 quality management system, with a per-lot Certificate of Analysis available on request.
Ultrapure Type 1 water (18.2 MΩ·cm) supports low trace-metal and organic-carbon background.

DCP-MEMGH-QPR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801) Available sizes: 500 mL, 1000 mL

Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Media familyMEM High Glucose + NEAA + 25mM HEPES
Glucose4500 mg/L (4.5 g/L, High Glucose)
HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAAPresent
Formulation[+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L)
[-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate
AppearancePale yellow-colored, clear solution (phenol red-free)
pH (USP <791>)7.4
Osmolality (USP <785>)290–330 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes mycoplasma, particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ addresses these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate primary cell media helps prevent chip channel clogging.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

HEPES: pH stability

25 mM HEPES helps limit pH drift during open-air suspension handling, flow cytometry prep, and multi-well assay setup outside CO₂ incubators.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin release specification — below the TLR4 activation threshold commonly cited for LPS-driven fibroblast activation and neuroinflammatory signaling in primary cultures.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis demand on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions.

1

0.1 µm Prefiltration I

Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and microaggregates, including the mycoplasma size range (0.2–0.3 µm), that pass a 0.22 µm filter.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter ahead of aseptic fill & finish in an ISO Class 5 (Class 100) environment.

Performance vs. conventional media

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size — sub-mycoplasma polishing

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma is controlled by 0.1 µm and 0.04 µm mycoplasma-retentive filtration at every production stage (not tested per lot).

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS™ DCP-MEMGH-QPR1X Minimum Essential Medium (MEM) High Glucose, NEAA and 25 mM HEPES ? Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish ? Microfluidics Suitable cell culture medium for organ-on-a-chip (OoC) and microphysiological system (MPS) applications | Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMGH-QPR1X

Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMGH-QPR1X is purpose-built for primary neurons and related cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic liquid-handling systems.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA with a defined glucose base is a physiologically relevant medium for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip

Epithelial Biology

Epithelium-on-Chip

MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip

Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts

Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate accumulation in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS

Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate filtration and a phenol red–free formulation reduce optical background for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors

Technical Specifications

Analytical release specifications

Available pack sizes: 500 mL, 1000 mL. Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Sodium Bicarbonate, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L) \| [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate
Appearance	Pale yellow-colored, clear solution (phenol red-free)
Glucose	4500 mg/L (4.5 g/L, High Glucose)
HEPES	25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA	Present
pH USP <791>	7.4
Osmolality USP <785>	290–330 mOsm/kg H₂O
Total ingredients	35

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	~5% CO₂ recommended (HEPES + sodium bicarbonate dual buffering; HEPES alone maintains pH without CO₂ for open-air handling)

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

MEM High Glucose + NEAA + 25mM HEPES: 35 ingredients released per lot with CAS numbers. NEAA components are listed under AMINO ACIDS. i-Inositol and HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) are listed under OTHERS.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	265.000
Magnesium sulfate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2200.000
Sodium chloride	7647-14-5	6800.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	122.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	7.500
L-Alanine	56-41-7	8.900
L-Arginine hydrochloride	1119-34-2	126.000
L-Asparagine monohydrate	5794-13-8	15.000
L-Aspartic acid	56-84-8	13.300
L-Cystine dihydrochloride	30925-07-6	31.300
L-Glutamic acid	56-86-0	14.700
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	52.000
L-Leucine	61-90-5	52.000
L-Lysine hydrochloride	657-27-2	72.500
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	32.000
L-Proline	147-85-3	11.500
L-Serine	56-45-1	10.500
L-Threonine	72-19-5	48.000
L-Tryptophan	73-22-3	10.000
L-Tyrosine disodium salt dihydrate	69847-15-0	51.900
L-Valine	72-18-4	46.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	1.000
D-Ca-Pantothenate	137-08-6	1.000
Folic acid	59-30-3	1.000
Nicotinamide	98-92-0	1.000
Pyridoxal hydrochloride	65-22-5	1.000
Riboflavin	83-88-5	0.100
Thiamine hydrochloride	67-03-8	1.000
OTHERS
i-Inositol	87-89-8	2.000
D-Glucose	50-99-7	4500.000
HEPES	7365-45-9	5958.000

Custom formulation: Contact support@diagnocine.com for DCP-MEMGH-QPR1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water supports trace-metal and organic-carbon (TOC) control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot released — no blending. Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290–330 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-MEMGH-QPR1X compares

FluxMPS™ DCP-MEMGH-QPR1X vs. conventional 0.22 µm–filtered MEM High Glucose + NEAA formulations.

Parameter	DCP-MEMGH-QPR1X (FluxMPS™)	Conventional MEM High Glucose (0.22 µm filtered)	Standard DMEM (0.22 µm filtered)
Grade	Microfluidics Suitable	Not designated	Not designated
MEM High Glucose + NEAA + 25mM HEPES — no L-glutamine, no pyruvate, no phenol red; researcher-defined metabolic control	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma-retentive filtration	check_circle Yes (0.1/0.04 µm)	cancel No	cancel No
Endotoxin (release specification)	FluxMPS™ — < 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
HEPES (25 mM) included	check_circle Yes	cancel Usually no	cancel No
NEAA included	check_circle Yes	Optional add-on	cancel No
USP <788> Method 1 particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle Yes — Microfluidics Suitable	cancel Higher clogging risk	cancel Higher clogging risk
Custom formulation available	check_circle Yes	cancel Typically no	cancel Typically no

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMGH-QPR1X.

Is DCP-MEMGH-QPR1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-MEMGH-QPR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA with mycoplasma-retentive filtration and an endotoxin release specification of < 0.05 EU/mL is well suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

Why are L-glutamine, sodium pyruvate, and phenol red all excluded from DCP-MEMGH-QPR1X, and how do I supplement?expand_more

Phenol red is excluded to reduce optical background for imaging; L-glutamine and sodium pyruvate are excluded to give researchers full control over nitrogen and carbon sources. High glucose (4.5 g/L) and NEAA provide the base energy and non-essential amino acid supply. HEPES (25 mM) together with sodium bicarbonate provides dual-buffer pH stability. Add L-glutamine (or a stable dipeptide substitute such as GlutaMAX) and sodium pyruvate at the concentrations required for your specific cell type; use a 0.2 µm low-protein-binding PES or PVDF filter for any supplement solution.

Does this medium require a CO₂ incubator?expand_more

Approximately 5% CO₂ is recommended. The formulation contains both sodium bicarbonate and 25 mM HEPES: bicarbonate buffering benefits from a CO₂ incubator, while HEPES alone can maintain pH during short periods of open-air handling outside CO₂.

Can DCP-MEMGH-QPR1X be supplemented with serum, growth factors, or other components?expand_more

Yes. Serum and other protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — never through a 0.04 µm membrane, which retains serum proteins and lipoproteins. Defined, protein-free additions may use a 0.1 µm filter.

What endotoxin level should I expect, and how is it verified?expand_more

DCP-MEMGH-QPR1X is produced to a release specification of < 0.05 EU/mL by LAL assay (USP <85>), controlled per manufacturing batch. Every batch is tested before release. For primary cells, endotoxin activates TLR4 signaling, which can influence fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function — which is why a low, batch-tested specification matters.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting MEM High Glucose + NEAA + 25mM HEPES in primary cell OoC applications.

Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Engineered for primary cell culture where standard media fails

Microchannel-safe purity

Primary cell–optimized formulation

HEPES: pH stability

Below TLR4 endotoxin threshold

NEAA reduces metabolic burden

Customization on demand

Quadruple-stage filtration system

0.1 µm Prefiltration I

0.04 µm Final filtration I

0.1 µm Prefiltration II

0.04 µm Final filtration II — Polish

Performance vs. conventional media

Primary cell models and OoC applications

Automated Bioreactors & Robotics

Neuronal Chips & Brain-on-Chip

Epithelium-on-Chip

Primary Fibroblast & Stromal Models

Vascular Cell Culture

Organ-on-a-Chip & MPS

Microscopy & Optical Sensing

Analytical release specifications

Full composition (mg/L)

Manufacturing & compliance

ISO 13485:2016 Quality Management

Ultrapure Type 1 Water

ISO Class 5 Fill & Finish

Micro-Batch Precision

Endotoxin — USP <85> BET

Particulate — USP <788> Method 1

Osmolality — USP <785>

Documentation & CoA

How DCP-MEMGH-QPR1X compares

Frequently asked questions

Supporting literature

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