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FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEMGH-QBR1X
$49.50
DCP-MEMGH-QBR1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-MEMGH-QBR1X is a Microfluidics Suitable, ultra-filtered MEM High Glucose + NEAA + 25mM HEPES formulation engineered for primary neurons and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells, and 25 mM HEPES (pKa 7.3 at 37°C) provides CO2-independent pH buffering. Formulation: [+] Earle's Salts (Calcium, Magnesium), [+] NEAA, [+] High Glucose (4500 mg/L), [+] Sodium Pyruvate, [+] 25mM HEPES | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red.

  • High Glucose (4.5 g/L) supports energy-demanding primary cell types.
  • NEAA pre-loaded — reduces metabolic burden and ammonia accumulation from de novo amino acid synthesis.
  • 25 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO2; suited to open-air handling, flow cytometry prep, and atmospheric incubation.
  • 0.04 µm final nano-filtration — sub-mycoplasma polishing; < 0.05 EU/mL endotoxin release specification for sensitive primary and hematopoietic cell cultures.
  • Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II.
  • Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill & finish.
  • Formulated without L-Glutamine, Sodium Bicarbonate, or Phenol Red — researcher-defined nitrogen source and imaging-clean, autofluorescence-reduced base.
DCP-MEMGH-QBR1X | Cell Culture Media
Available sizes: 500 mL, 1000 mL
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose, NEAA & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Media familyMEM High Glucose + NEAA + 25mM HEPES
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • NEAAPresent
  • Formulation[+] Earle's Salts (Ca, Mg), [+] NEAA, [+] High Glucose, [+] Sodium Pyruvate, [+] 25mM HEPES
    [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
  • AppearancePale yellow, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235–275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes mycoplasma, subvisible particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ addresses these failure modes through validated filtration while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Low-particulate primary cell media helps prevent chip channel clogging.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: the established base for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

HEPES: CO2-stable pH

25 mM HEPES helps prevent pH drift during open-air suspension handling, flow cytometry prep, and multi-well assay setup outside CO2 incubators.

shield

Low-endotoxin release specification

< 0.05 EU/mL endotoxin release specification, tested by LAL assay every batch. Endotoxin is a known TLR4 agonist; a low release specification helps reduce the risk of LPS-driven fibroblast activation, neuroinflammatory signaling, and epithelial barrier disruption in primary cell assays.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions. The train is a repeated prefilter-plus-final-filter pair, run twice: each 0.04 µm final filter has its own dedicated 0.1 µm prefilter.

  1. 1

    0.1 µm Prefiltration I — Large Particulate Removal

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge; mycoplasma-retentive grade (validated with A. laidlawii).

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate sub-mycoplasma polishing filter; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm mycoplasma-retentive filtration (0.2–0.3 µm organism diameter) applied at every production run; this is not a per-lot mycoplasma assay result.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEMGH-QBR1X Minimum Essential Medium MEM High Glucose NEAA and 25mM HEPES without L-Glutamine, Sodium Bicarbonate, Phenol Red 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM High Glucose plus NEAA plus 25mM HEPES for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEMGH-QBR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEMGH-QBR1X is purpose-built for primary neurons and related primary cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for automated bioreactor and robotic handling systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates below the 0.04 µm cut-off.
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries.
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture.

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle's Salts + NEAA is a widely used base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

MEM with NEAA supports primary epithelial cells and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle's salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

This ultra-low particulate, phenol red–free formulation reduces phenol red–derived optical interference, benefiting confocal imaging, TEER sensor readings, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts (Ca, Mg), [+] NEAA, [+] High Glucose (4500 mg/L), [+] Sodium Pyruvate, [+] 25mM HEPES | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow, clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 235–275 mOsm/kg H2O
Total ingredients 35
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — 25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.4 without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM High Glucose + NEAA + 25mM HEPES: 35 ingredients verified per lot with CAS numbers. NEAA components are listed under AMINO ACIDS. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
HEPES 7365-45-9 5958.000
Sodium Pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-MEMGH-QBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feedwater, controlled for trace metals and organic carbon.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), light obscuration.

Osmolality — USP <785>

Target: 235–275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEMGH-QBR1X compares

FluxMPS™ DCP-MEMGH-QBR1X vs. conventional 0.22 µm–filtered MEM High Glucose + NEAA formulations.

Parameter DCP-MEMGH-QBR1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm filtered) Standard DMEM (0.22 µm)
Grade Microfluidics Suitable (0.04 µm final cut-off) Not specified Not specified
MEM High Glucose + NEAA + HEPES-only, CO2-free — no L-Glutamine, no Phenol Red; imaging-clean, atmospheric handling check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration (0.1 µm) check_circle Yes cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
HEPES (25 mM) included check_circle Yes cancel Usually no cancel No
NEAA included check_circle Yes Optional add-on cancel No
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEMGH-QBR1X.

Yes. DCP-MEMGH-QBR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low-particulate media for MPS, OoC, and LoC platforms. MEM + Earle's Salts + NEAA is well suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms.
 
HEPES provides CO2-independent buffering, so sodium bicarbonate is not needed; phenol red is omitted to reduce optical interference for imaging and biosensor work. L-Glutamine is left out so researchers can add their preferred nitrogen source (for example, 2 mM L-Glutamine or a stable dipeptide substitute) at the concentration appropriate to their cell type. Sodium Pyruvate and high glucose are already included to support energy-demanding primary cells.
No. This formulation is CO2-independent — 25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.4 without gas supplementation, making it suitable for open-bench handling and atmospheric incubation.
Yes. Serum and other protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — never through a 0.04 µm membrane, which retains IgM, VLDL, chylomicrons, and much of the lipid/lipoprotein fraction of serum. Defined, protein-free additions may use a 0.1 µm filter.
Every batch of DCP-MEMGH-QBR1X is produced to meet a release specification of < 0.05 EU/mL, tested by LAL assay (USP <85> Bacterial Endotoxins Test, assay sensitivity 0.005 EU/mL) before release. This is a batch-level specification, not a per-unit test result.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM High Glucose + NEAA + 25mM HEPES in primary cell and OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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