FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- High glucose (4.5 g/L, 4500 mg/L) formulation with Non-Essential Amino Acids (NEAA) — supports high-energy-demand cell types
- Quadruple-stage filtration train: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II) — two dedicated prefilter/final-filter pairs run in series
- Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> BET), tested per manufacturing batch
- pH 7.4 (USP <791>); osmolality reported per lot on the Certificate of Analysis
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
- ISO Class 5 aseptic fill & finish under an ISO 13485:2016 quality management system
- Sodium bicarbonate (2200 mg/L) buffered formulation with a phenol red pH indicator — requires a CO2 incubator
- Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request via support@diagnocine.com
- Glucose4500 mg/L (4.5 g/L)
- L-Glutamine292 mg/L
- Sodium Pyruvate110 mg/L
- HEPESNot added
- NEAAIncluded
- pH (USP <791>)7.4
- Osmolality (USP <785>)Reported per lot (see CoA)
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and lot-to-lot variability that accumulate inside microchannels — corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ addresses these failure modes through a validated four-pass filtration train.
Microchannel-safe purity
0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate testing is performed per lot.
High-glucose metabolic support
4.5 g/L glucose supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.
Ultrapure-grade water
Prepared with Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696), controlling trace metals and organic carbon in the feed water.
Low background for imaging
Quadruple-stage filtration delivers a low particulate baseline suited to confocal microscopy, live-cell biosensors, and TEER measurements.
Rich, stable nutrient profile
NEAA-supplemented formulation plus micro-batch precision manufacturing ensures comprehensive amino acid coverage for demanding cell culture models.
Customization on demand
pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA: 1X Liquid is processed through a four-pass filtration train — two dedicated prefilter/final-filter pairs run in series — reaching a 0.04 µm final cut-off, addressing mycoplasma-sized particulates and subvisible debris that 0.22 µm filtration cannot retain.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final-filter cartridge.
-
2
0.04 µm Final filtration I
Retains sub-micron particulates and microaggregates, including mycoplasma-sized organisms (0.2–0.3 µm diameter), that pass through a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge and providing redundancy against breakthrough.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill performed in a validated ISO Class 5 laminar-flow workstation.
Performance vs. conventional media
Two prefilter/final-filter pairs in series, reaching a 0.04 µm final cut-off, deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration.
© Diagnocine® — DCP-MEMG1X
Designed for next-generation cell culture platforms
FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA: 1X Liquid is suited to organ-on-a-chip, cancer biology, stem cell, vascular, metabolic, and live-cell imaging research where particulate load and batch-to-batch variation must be minimized.
Automated Bioreactors & Robotics
A separately designated MPS Grade variant, ultra nano-filtered to 0.01 µm (10 nm) with additional 0.02 µm and 0.01 µm stages after the 0.04 µm polish, is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.
- Total Particulate Exclusion: 0.01 µm (10 nm) filtration for nanoscale valve and sensor protection
- Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
- Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs
Inquiry Required: Contact support@diagnocine.com to request the MPS Grade, 0.01 µm variant.
Micro Physiological System (MPS) & Chip
Quadruple-stage filtered formulation supports microchannel flow integrity across complex chip geometries.
Warburg Effect & Metabolic Research
High-glucose base supports Warburg effect studies and glucose uptake assays in cancer cell lines.
iPSC-Derived Models
Low-particulate formulation supports sensitive iPSC differentiation protocols where particulate load causes off-target effects.
Endothelial & Primary Cells
Microchannel-safe purity supports endothelial barrier integrity and TEER measurements in perfusion models.
Metabolic Flux Analysis
High-glucose base enables precise 13C isotope tracing and glycolytic flux experiments.
Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Low particulate load supports high-content confocal imaging and optical biosensor integration.
Lot-release quality parameters
Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA: 1X Liquid undergoes the quality-release battery below before shipment.
| Parameter | Specification |
|---|---|
| Formulation | L-Glutamine (292 mg/L), Sodium Bicarbonate (2200 mg/L), Phenol Red (11 mg/L), Calcium (as CaCl2·2H2O, 265 mg/L), Magnesium (as MgSO4, 97.72 mg/L), Glucose (4500 mg/L), Sodium Pyruvate (110 mg/L); HEPES not added |
| Appearance | Orange-to-red colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Reported per lot (see CoA) |
| Glucose | 4500 mg/L (4.5 g/L) |
| L-Glutamine | 292 mg/L |
| Sodium Pyruvate | 110 mg/L |
| Phenol Red | 11 mg/L (present) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification — see §Manufacturing & Compliance) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | Compliant (lot-specific data in CoA) |
| Particulate ≥25 µm USP <788> Method 1 | Compliant (lot-specific data in CoA) |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696) |
| Manufacturing std. ISO 13485 | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Not recommended |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | Approximately 5% CO2 (derived from 2,200 mg/L sodium bicarbonate buffering) |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot documentation, CoA available |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch precision manufacturing |
| Intended use | For Research Use Only (RUO) |
| Available pack sizes | 500 mL, 1000 mL |
Full composition (mg/L)
Complete formulation with CAS numbers, reproduced from manufacturer specification. Total: 37 components across 4 formulation categories (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 browsable tabs below. Custom compositions available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2200.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.900 |
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Asparagine monohydrate | 15.000 | |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30189-89-0 | 31.300 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 51.900 | |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 2.000 |
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
ISO 13485:2016 manufacturing & compliance
Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering manufacturing, testing, and release for every production lot.
Ultrapure Type 1 Water
All media prepared with 18.2 MΩ·cm resistivity water (ASTM D1193 / ISO 3696).
ISO Class 5 Fill & Finish
Aseptic filling in a validated ISO Class 5 laminar-flow workstation; 21 CFR Part 820 (QMSR) aligned.
Micro-Batch Precision
Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay on every batch. Release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.
Osmolality — USP <785>
Freezing-point osmometry per USP <785>. Result reported per lot on the CoA.
Documentation — CoA & Full Lot Records
Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.
How DCP-MEMG1X (FluxMPS™) compares
Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives.
| Parameter | DCP-MEMG1X (FluxMPS™) | Conventional MEM High Glucose (0.22 µm) | Standard MEM High Glucose alternative |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not designated | Not designated |
| Base Formulation | Minimum Essential Medium (MEM), High Glucose, NEAA: 1X Liquid | MEM High Glucose Standard | MEM High Glucose Equivalent |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 passes | 1 pass | 1–2 passes |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Variable | Variable |
| Microfluidic channel compatibility | check_circle Validated | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle On request | cancel | Limited |
Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose, NEAA: 1X Liquid.
Supporting literature
Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.
- Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
- Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
- Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
- Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
- Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
- Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
- Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
- Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
- Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
