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FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-MEMGH-PBN1X
$44.00
DCP-MEMGH-PBN1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Glucose (4.5 g/L) Without Sodium Bicarbonate Without Sodium Pyruvate

FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. The bicarbonate-free, HEPES-buffered, high-glucose (4.5 g/L) formulation reaches a 0.04 µm final pore size across four validated filtration passes, keeping microfluidic channels and biosensor surfaces free of the subvisible particulate load carried by conventional 0.22 µm-filtered media.

  • High-glucose (4.5 g/L) Minimum Essential Medium formulated with 25 mM HEPES for microfluidic and OoC perfusion systems
  • Quadruple-stage filtration: 0.1 µm ×2 + 0.04 µm ×2, four validated passes reaching a 0.04 µm final cut-off
  • Sodium-bicarbonate-free, HEPES-buffered formulation; reduced CO2 dependence — validate per cell line
  • No sodium pyruvate added; 4.5 g/L glucose serves as the primary carbon source
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC and customization at Diagnocine, Totowa, NJ
  • Phenol red present (11 mg/L) for visual pH monitoring — not compatible with phenol-red-free optical assay platforms
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEMGH-PBN1X | Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L)
  • L-Glutamine292 mg/L
  • Sodium PyruvateNot added
  • HEPES5958 mg/L (25 mM)
  • Sodium BicarbonateNot added
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months from manufacture
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm diameter), subvisible debris, and variable endotoxin load that accumulate inside microchannels — corrupting biosensor readings, stressing primary cultures, and shortening device lifetimes. FluxMPS™ is built around a filtration process designed to address these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filter, reached across four validated passes; USP <788> Method 1 (light obscuration) particulate testing performed per lot.

target

High-glucose metabolic support

4.5 g/L glucose supports high-energy-demand cell types including HeLa, MCF-7, fibroblasts, and iPSC-derived models in perfusion devices.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm), controlling trace-metal and organic-carbon (TOC) background in the finished medium.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy, live-cell biosensor readouts, and TEER measurements where particulate interference would confound results.

science

Rich, stable nutrient profile

Thirteen essential amino acids at standard Minimum Essential Medium concentrations, plus a full B-vitamin panel, held to micro-batch lot-to-lot consistency.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is processed through a four-pass filtration train — two prefilter-plus-final-filter pairs in series — reaching a 0.04 µm final pore size.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a 0.22 µm filter unchecked.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge for a fully redundant filtration path.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish performed in a validated laminar-flow workstation.

Filtration architecture

Two prefilter-plus-final-filter pairs run in series, each 0.04 µm final filter protected by its own dedicated 0.1 µm prefilter, reaching a 0.04 µm final pore size with USP <788> Method 1 particulate testing performed on every production lot.

4
Validated filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Minimum Essential Medium (MEM) High Glucose 25mM HEPES DCP-MEMGH-PBN1X Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) for Microfluidics Suitable cell culture media.
© Diagnocine® — DCP-MEMGH-PBN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is validated for organ-on-a-chip, cancer biology, stem cell, vascular, metabolic, and live-cell imaging models where particulate load and endotoxin variability are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered variant — Diagnocine's separate MPS Grade line — is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 0.01 µm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Quadruple-stage filtered formulation supports microchannel flow paths and helps maintain laminar flow integrity across chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High-glucose (4.5 g/L) base supports Warburg effect studies and glucose uptake assays in cancer cell lines.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered formulation supports sensitive iPSC differentiation protocols where particulate and endotoxin variability cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurement in perfusion models.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

High-glucose base enables 13C isotope tracing and glycolytic flux experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load supports high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid undergoes the quality-release battery below before shipment. Available pack sizes: 500 mL, 1000 mL.

Physical & Chemical Parameters
Parameter Specification
Formulation MEM, High Glucose, 25 mM HEPES; L-Glutamine, Phenol Red, Calcium and Magnesium present; Sodium Pyruvate and Sodium Bicarbonate not added
Appearance Orange-to-red colored, clear solution (phenol red present)
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 4500 mg/L (4.5 g/L)
L-Glutamine 292 mg/L
Sodium Pyruvate Not added
Phenol Red 11 mg/L (present)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate USP <788> Method 1 Light obscuration; compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100) laminar-flow workstation
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement HEPES-buffered; reduced CO2 dependence (validate per cell line)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Total: 29 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others). Complete formulation with CAS numbers, reproduced in full from the manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 11.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system with final packaging, testing, and customization at Diagnocine, Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

Media prepared with 18.2 MΩ·cm resistivity water, controlling trace-metal and organic-carbon background.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in a validated ISO Class 5 laminar-flow workstation; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms particulate compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: see Certificate of Analysis.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com.
Product Comparison

How DCP-MEMGH-PBN1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered MEM High Glucose alternatives.

Parameter DCP-MEMGH-PBN1X (FluxMPS™) Conventional MEM High Glucose (0.22 µm) Standard MEM High Glucose alternative
Grade Microfluidics Suitable Not applicable Not applicable
Base Formulation MEM, High Glucose, 25 mM HEPES, w/o Sodium Pyruvate, w/o Sodium Bicarbonate MEM High Glucose Standard MEM High Glucose Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 passes 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid.

Yes. DCP-MEMGH-PBN1X is processed through our Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), reaching a 0.04 µm final pore size that reduces the particulate load reaching OoC and MPS microchannels compared to conventional 0.22 µm-filtered media.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or fine subvisible particulates. FluxMPS™ uses four sequential passes — two 0.1 µm prefilter and 0.04 µm final-filter pairs — reaching a 0.04 µm final cut-off, with USP <788> Method 1 (light obscuration) particulate testing performed per lot.
This formulation is HEPES-buffered and bicarbonate-free by design, and does not include sodium pyruvate. If your protocol requires sodium bicarbonate for CO2-buffered incubation, or sodium pyruvate as a supplemental carbon/redox source, both can be added at time of use, or contact support@diagnocine.com for a custom pre-formulated batch.
This formulation is HEPES-buffered and bicarbonate-free, giving it reduced CO2 dependence. Buffering performance should be validated for your specific cell line and incubation setup.
Yes. FluxMPS™ Minimum Essential Medium (MEM), High Glucose & 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid can be supplemented with FBS, growth factors, antibiotics, or other additives per standard practice. When filtering serum-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF filter — never a 0.04 µm filter, which retains serum proteins and lipoproteins. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> BET (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before shipment. Batch-specific results are documented in the Certificate of Analysis, available from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com with your lot number.
Scientific References

Supporting literature

Peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  8. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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