MagExtractor -PCR & Gel Clean up

Product#: NPK-601
$254.50
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MagExtractor -PCR & Gel Clean up- High Efficient DNA Fragment purification Kit

Cat.No. NPK-601
Storage Store at 4ºC


Description


MagExtractor-PCR & Gel Clean up- provides a simple and reliable method for the rapid purification of DNA fragments from a PCR solution, enzyme solution, or agarose gel slices utilizing magnetic silica beads. This kit is based on binding properties of DNA to a silica surface in the presence of chaotropic agents (1). The purified DNA fragments can be used directly for general molecular biology experiments.

Features
  • Extracts DNA fragments from ≤ 100 µL PCR solutions or enzyme solutions within 5 minutes.
  • Extracts DNA fragment from ≤ 0.3 g agarose gel slices (TAE or TBE) within 15 minutes.
  • Agarose slices can be melted at room temperature.
  • Typical yields from solution or gel slices are approximately 60-70%.
  • DNA fragments of approximately 100 bp to 50 kb can be effectively recovered. Small fragments ( < 40 bp) can   be removed.
  • Purified DNA fragments can be applied to sequencing, restriction enzyme treatment, labeling, ligation, transformation, etc.

Applications
  • Purification of DNA fragments from various solutions
  • Purification of DNA fragments from an agarose gel slice
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Components

Binding Solution               88 mL
Washing Solution           132 mL
Magnetic Beads              8.5 mL


Principle

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The selectivity of extracted nucleic acids can be changed by optimization of the binding and washing solutions. MagExtractor -Genome- (Code No. NPK-101) extracts genomic DNA from various specimens (e.g. whole blood, cultured cells or animal tissues etc.). MagExtractor -RNA- (Code No. NPK-201) extracts total RNA from various specimens (e.g. cultured cells or animal tissues). MagExtractor -Plasmid- (Code No. NPK-301) extracts plasmids from E. coli cells, MagExtractor -Viral RNA- (Code No. NPK-401) is a kit for extracting viral RNA from serum or plasma specimens. MagExtractor -Plant Genome- (Code No. NPK-501) is a kit for extracting genomic DNA from various plant specimens (e.g., leaf, cultured cells, etc.). MagExtractor-PCR & Gel Clean up- (Code No. NPK-601) extracts DNA fragments from a PCR solution, enzyme solution, or agarose gel slices.

Application Data
 
Example 1. Purification of DNA fragments from DNA solutions
DNA fragments were purified from PCR products (120 bp, 1 kb, 2kb) and φx174/Hinf I DNA size markers (0.25 µg/µL) <100 µL> by MagExtractor –PCR & Gel clean up–. The purified DNA fragments were analyzed by agarose and acrylamide gels, respectively. As shown in Fig.1, the fragments approx. > 60 bp could be recovered with high yield. On the other hand, the < 50 bp fragments and primer dimers were removed efficiently (Fig. 2).
 
pic_03 (1).png Fig. 1. Electrophoretic analysis of the purified PCR products from solutions.

1,3,5: Before purification
2,4,6: After purification
pic_04.png Fig. 2. Electrophoretic analysis of the purified fx174/Hinf I DNA size markers from solutions.

M: φx174/Hinf I Marker
1: MagExtractor
2: Company A
3: Company B
4: Company C
   
Example 2. Purification of DNA fragments from agarose gel slices
DNA fragments were purified from PCR products (120 bp, 1 kb, 2kb) and λ phage DNA (48.5 kb) from gel slices by MagExtractor -PCR & Gel clean up- and other companies’ purification kits. As shown in Fig. 1 and 2, all fragments could be recovered with high yield using MagExtractor -PCR & Gel clean up-. Other companies’ purification kits showed poor yields in the case of the large DNA fragment (48.5 kb) (Fig. 2).
 
pic_05.png Fig. 1. Electrophoretic analysis of the purified PCR products from gel slices.

1,3,5: Before purification
2,4,6: After purification
pic_06.png Fig. 2. Electrophoretic analysis of the purified λ phage DNA (48.5 kb) from gel slices.

M: λ/HindIII Marker
1: Before purification
2: MagExtractor
3: Company A
4: Company B
 
References 
  1. B. Vogelstein and D. Gillespie, Proc. Natl. Acad. Sci. USA. 76: 615-619 (1979)
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