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FluxMPS™ MCDB 302 Medium: 1X Liquid

Product#: DCP-M3021X
$71.49
DCP-M3021X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ MCDB 302 Medium: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate

FluxMPS™ DCP-M3021X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MCDB 302 Nucleoside-Free formulation engineered for serum-free or low-protein culture of Chinese Hamster Ovary (CHO) cells under nucleoside-free conditions for DHFR-mediated gene amplification selection, and for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The formulation supplies trace elements — zinc, copper, selenium and manganese — as dissolved, particle-free ions through the 0.04 µm final filter stage, with a per-batch endotoxin release specification of < 0.05 EU/mL.

  • Nucleoside-free formulation supports DHFR-mediated gene amplification selection in CHO cell lines
  • Trace element profile — zinc, copper, selenium, manganese — for metalloenzyme support and antioxidant activity in serum-free culture
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), verified per manufacturing batch
  • Formulated with 1.801 g/L glucose, L-glutamine, sodium pyruvate and sodium bicarbonate; phenol red present
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, glucose, trace element and growth-factor co-formulations available on request
CAT. NO.
DCP-M3021X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
MCDB 302 Medium: 1X Liquid
  • Glucose1801 mg/L (1.801 g/L)
  • L-Glutamine438.6 mg/L
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280–320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack, 2–8°C in transit
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

MCDB media formulated with trace element salts can form subvisible particulate aggregates when filtered at 0.22 µm under typical manufacturing conditions. These aggregates clog microfluidic channels and can affect trace metal bioavailability in sensitive serum-free cultures. FluxMPS™ addresses these failure modes with four-stage sub-0.04 µm filtration and a < 0.05 EU/mL endotoxin release specification.

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Particle-free trace element delivery

0.04 µm final filtration helps ensure trace metal salts remain fully dissolved, reducing the risk of aggregate deposition in chip channels or culture vessels.

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Serum-free primary cell support

Trace element composition supports metalloenzyme activity (SOD, GPx, carbonic anhydrase) relevant to serum-free cell survival and function.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) is used in manufacturing to control trace-metal and organic-carbon background in the finished medium.

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Low-endotoxin formulation

< 0.05 EU/mL batch release specification (LAL, USP <85>) helps reduce the risk of endotoxin-driven inflammatory signaling in sensitive serum-free cultures.

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Rich nutrient profile

MCDB 302 trace element and amino acid profile supports clonal growth and serum-free cell survival.

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Customization on demand

pH, glucose, trace element concentrations, HEPES, and growth-factor co-formulations available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish, delivering low-particulate, mycoplasma-retentive purity for trace-element-containing MCDB formulations used in microfluidic and organ-on-a-chip platforms.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and aggregates, including trace metal salt precipitates; protects the first 0.04 µm cartridge downstream.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that would pass a standard 0.22 µm filter, including material in the mycoplasma size range (0.2–0.3 µm).

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm cartridge; this is not polishing the first filter’s effluent, it is safeguarding the next stage.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill in a validated ISO Class 5 (Class 100) environment.

Performance vs. conventional media

FluxMPS™ DCP-M3021X is processed through two prefilter + final-filter pairs (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm), reaching a 0.04 µm final cut-off — substantially finer than the 0.22 µm filtration used in conventional MCDB 302 Nucleoside-Free preparations.

4
Filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final pore size
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved via 0.1 µm mycoplasma-retentive filtration (not tested per lot); typical mycoplasma diameter is 0.2–0.3 µm.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-M3021X MCDB 302 Medium: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II ? Polish ? Microfluidics Suitable MCDB 302 Nucleoside-Free for organ-on-a-chip and CHO DHFR selection | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) supporting particle-free trace element delivery.
© Diagnocine® — DCP-M3021X
Applications

Specialized cell models & OoC applications

FluxMPS™ DCP-M3021X was formulated for Chinese Hamster Ovary (CHO) cells under nucleoside-free conditions for DHFR-mediated selection, in low-protein or serum-free culture. The 0.04 µm filtered trace element matrix is intended for use in microfluidic chip architectures where conventional MCDB media may cause trace metal particulate fouling.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available for automated bioreactor perfusion applications where sub-0.04 µm trace metal salt colloids may contribute to valve fouling.

  • Trace Metal Nanoparticulate Removal: 0.01 µm filtration targets sub-0.04 µm metal salt colloids not addressed by standard QC
  • Valve & Sensor Protection: Intended to reduce micro-fouling risk from trace element aggregates in automated systems
  • Extended Perfusion Stability: Supports consistent trace element delivery over extended perfusion culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

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Organ-on-a-Chip & MPS

0.04 µm filtration is intended to reduce microchannel clogging risk. Trace elements are delivered as dissolved ions rather than particulate aggregates.

CHO cells — nucleoside-free, DHFR selection
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CHO Bioproduction

FluxMPS™ MCDB 302 Nucleoside-Free supports low-particulate, trace-element-defined culture for CHO bioproduction models in serum-free or low-protein conditions.

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DHFR-Mediated Selection

Nucleoside-free formulation supports DHFR selection and methotrexate step-selection workflows for gene amplification in CHO-DHFR-deficient cell lines.

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CHO-on-Chip

Low-particulate, mycoplasma-retentive-filtered formulation suited to microfluidic CHO culture models.

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Biopharmaceutical Cell Culture

Trace element and amino acid profile supports biopharmaceutical CHO cell culture workflows in serum-free or low-protein conditions.

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Metalloenzyme-Dependent Assays

Zinc, copper, selenium and manganese support metalloenzyme-dependent assays (SOD, GPx) in serum-free primary and CHO cell models.

Technical Specifications

Analytical release specifications

Every lot released against the specification matrix below. Pack sizes: 500 mL, 1000 mL. Certificate of Analysis: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate
Appearance Red-colored (phenol red present), clear solution
pH USP <791> 7.4
Osmolality USP <785> 280–320 mOsm/kg H2O
Glucose 1801 mg/L (1.801 g/L)
L-Glutamine 438.6 mg/L
Sodium Pyruvate 110 mg/L
Phenol Red 1.242 mg/L (phenol red sodium salt)
Trace Elements Present (zinc, copper, selenium, manganese, molybdenum, vanadium)
Nucleosides Not added (nucleoside-free for DHFR-mediated selection)
Total ingredients 50 (13 inorganic salts, 20 amino acids, 9 vitamins, 8 others)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification — see Manufacturing & Compliance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack, 2–8°C in transit
CO2 requirement Approximately 3% CO2 (derived from ~14 mM sodium bicarbonate at pH 7.4; validate empirically per incubator and cell line)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MCDB 302 Nucleoside-Free: 50 ingredients verified per lot with CAS numbers for raw-material traceability, including trace elements for metalloenzyme support and antioxidant activity.

Component CAS Number mg/L
INORGANIC SALTS
Ammonium metavanadate 7803-55-6 0.00117
Calcium chloride dihydrate 10035-04-8 88.210
Cupric sulphate pentahydrate 7758-99-8 0.0025
Disodium hydrogen phosphate anhydrous 7558-79-4 141.980
Ferrous sulphate heptahydrate 7782-63-0 0.834
Magnesium chloride hexahydrate 7791-18-6 122.000
Manganese sulphate 7785-87-7 0.000151
Molybdic acid ammonium tetrahydrate 7785-87-7 0.0124
Potassium chloride 7447-40-7 223.650
Sodium bicarbonate 144-55-8 1180.00
Sodium chloride 7647-14-5 7599.000
Sodium selenite 10102-18-8 0.00173
Zinc sulphate heptahydrate 7446-20-0 0.863
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.510
L-Alanine 56-41-7 8.910
L-Arginine hydrochloride 1119-34-2 210.700
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.310
L-Cysteine hydrochloride monohydrate 7048-04-6 17.560
L-Glutamic acid 56-86-0 14.710
L-Glutamine 56-85-9 438.600
L-Histidine hydrochloride monohydrate 5934-29-2 20.970
L-Isoleucine 73-32-5 3.940
L-Leucine 61-90-5 13.120
L-Lysine hydrochloride 657-27-2 36.540
L-Methionine 63-68-3 4.480
L-Phenylalanine 63-91-2 4.960
L-Proline 147-85-3 34.530
L-Serine 56-45-1 10.510
L-Threonine 72-19-5 11.910
L-Tryptophan 73-22-3 2.040
L-Tyrosine disodium salt dihydrate 69847-15-0 7.896
L-Valine 72-18-4 11.720
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 13.960
D-Biotin 58-85-5 0.00733
D-Ca-Pantothenate 137-08-6 0.238
Folic acid 59-30-3 1.324
Niacinamide 98-92-0 0.0366
Pyridoxine hydrochloride 58-56-0 0.0617
Riboflavin 83-88-5 0.0376
Thiamine hydrochloride 67-03-8 0.337
Vitamin B12 68-19-9 0.136
OTHERS
myo-Inositol 87-89-8 18.020
D-Glucose 50-99-7 1801.600
Hypoxanthine 68-94-0 4.083
Linoleic acid 60-33-3 0.0841
Phenol red sodium salt 34487-61-1 1.242
Putrescine dihydrochloride 333-93-7 0.161
Sodium pyruvate 113-24-6 110.000
Thioctic acid 1077-28-7 0.206
Custom formulation: Growth factor co-formulations, adjusted trace element concentrations, custom glucose, and pH modifications available. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular attention to trace element dissolution and particulate control during MCDB formulation.

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ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm process water used for trace-element-containing MCDB formulations.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

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Micro-Batch Precision

Small-batch production with per-lot traceability and a Certificate of Analysis for every lot, including trace element verification against specification.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280–320 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-M3021X compares

FluxMPS™ DCP-M3021X vs. conventional 0.22 µm–filtered MCDB 302 Nucleoside-Free formulations.

Parameter DCP-M3021X (FluxMPS™) Conventional MCDB 302 Nucleoside-Free
(0.22 µm filtered)		 Standard Alt. MCDB
(0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
MCDB 302 Nucleoside-Free with Trace Elements — CHO DHFR selection & bioproduction OoC check_circle Yes cancel No cancel No
Trace element delivery Particle-free (0.04 µm) May contain aggregates May contain aggregates
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stage) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-M3021X MCDB 302 Nucleoside-Free.

Yes. DCP-M3021X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, intended to provide low-particulate media for MPS, OoC, ToC and LoC platforms. The trace element profile is delivered as dissolved ions through the 0.04 µm final filter, and endotoxin is controlled to a release specification of < 0.05 EU/mL, tested per manufacturing batch.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — Polish — reaching a 0.04 µm final cut-off versus 0.22 µm for conventional MCDB media. For trace-element-containing formulations, this additional filtration helps ensure trace metal salts remain dissolved rather than depositing as micro-aggregates in microfluidic channels.
Nucleosides (such as thymidine and hypoxanthine) are excluded to create the nucleoside-free conditions required for DHFR (dihydrofolate reductase) selection. In the DHFR amplification system, cells lacking endogenous DHFR cannot synthesize nucleosides de novo — only cells that have integrated and expressed the DHFR transgene survive in nucleoside-free medium. This is the standard selection pressure used to amplify recombinant protein expression in CHO DHFR-deficient cell lines via methotrexate (MTX) step selection. Hypoxanthine appears in the composition table (4.083 mg/L) as a formulation component rather than a supplement — do not add additional nucleosides unless selection pressure is intentionally being removed.
Yes. This formulation is buffered with sodium bicarbonate (approximately 14 mM). Based on the Henderson-Hasselbalch relationship, maintaining pH 7.4 at this bicarbonate concentration requires approximately 3% CO2. Validate the exact incubator set point empirically for your specific vessel and cell line.
Yes. MCDB media are designed for low-protein or serum-free conditions but can be supplemented with growth factors (EGF, bFGF, VEGF, etc.), dialyzed FBS protein (FBSP, 0.5–2%), antibiotics, or custom nutrients. Serum and protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane prior to addition — 0.04 µm membranes are not appropriate for protein-containing additions and will strip serum of active components. Contact support@diagnocine.com for custom growth-factor co-formulations.
DCP-M3021X is produced to a release specification of < 0.05 EU/mL, tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) on a per-manufacturing-batch basis. Endotoxin activates TLR4-mediated inflammatory signaling in many cell types, so controlling this specification is relevant to reproducibility in serum-free and primary cell culture. A Certificate of Analysis reporting the tested value is available per lot.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MCDB 302 Nucleoside-Free CHO culture and organ-on-a-chip applications.

  1. Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-6
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216–4220. doi:10.1073/pnas.77.7.4216
  6. Kaufman RJ, Sharp PA. Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene. J Mol Biol. 1982;159:601–621. doi:10.1016/0022-2836(82)90103-6
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
  9. Wurm FM. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol. 2004;22:1393–1398. doi:10.1038/nbt1026

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