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FluxMPS™ MCDB 201 Medium w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-M201H-B1X
$71.49
DCP-M201H-B1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ MCDB 201 Medium w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES (30 mM) Contains Calcium Contains Magnesium Contains Glucose (1.441 g/L) Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-M201H-B1X is a Microfluidics Suitable, quadruple-stage ultra-filtered MCDB 201 −Bicarbonate +HEPES formulation engineered for serum-free or low-protein culture of Chick Embryo Fibroblasts, mesenchymal stem cells (MSCs) and primary fibroblasts on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Sodium bicarbonate is fully removed; 30 mM HEPES alone maintains pH, making the medium CO2-independent.

  • Trace element formulation — zinc, copper, selenium, manganese, molybdenum, vanadium and nickel salts for metalloenzyme support and antioxidant activity in serum-free culture
  • Formulated for Chick Embryo Fibroblasts, mesenchymal stem cells (MSCs) and primary fibroblasts under CO2-independent, HEPES-buffered conditions
  • 30 mM HEPES (pKa 7.3 at 37°C) replaces sodium bicarbonate entirely, maintaining stable pH without gas-phase CO2 control — suited to open-top chips and atmospheric platforms
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish for particle-free trace element delivery and mycoplasma-retentive filtration
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch with Certificate of Analysis on request
  • Manufactured under an ISO 13485:2016 quality management system, with final QC performed at Diagnocine’s Totowa, NJ facility
  • 53 formulation components verified per lot — 14 inorganic salts, 20 amino acids, 10 vitamins and 9 additional components — with full raw-material traceability
  • Custom pH, glucose, trace element and HEPES concentrations available on request — contact support@diagnocine.com
CAT. NO.
DCP-M201H-B1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
MCDB 201 Medium w/o Sodium Bicarbonate: 1X Liquid
  • Cell typesChick Embryo Fibroblasts, MSCs, Primary Fibroblasts
  • Glucose1441 mg/L (1.441 g/L)
  • HEPES30 mM (pKa 7.3 at 37°C)
  • Trace ElementsZinc, copper, selenium, manganese and others
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280 - 320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

MCDB media formulated for primary cells contain trace element salts that can form subvisible particulate aggregates when filtered at 0.22 µm under typical manufacturing conditions. These aggregates can deposit in microfluidic channels, disrupt trace metal bioavailability, and contribute to innate immune signaling in sensitive primary cell models. FluxMPS™ addresses these failure modes with four-stage sub-0.04 µm filtration and a < 0.05 EU/mL endotoxin release specification.

filter_alt

Particle-free trace element delivery

0.04 µm final filtration helps ensure trace metal salts remain dissolved rather than depositing as aggregates in chip channels or culture vessels, where precipitation could alter trace metal bioavailability.

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Serum-free primary cell support

The trace element composition supports metalloenzyme activity (SOD, GPx, carbonic anhydrase) relevant to primary cell survival and function in low-serum or serum-free conditions.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) minimizes ionic contaminants in a formulation where zinc, copper and selenium uptake by low-serum primary cells is sensitive to background ionic load.

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Batch-tested endotoxin specification

Every manufacturing batch is released against a < 0.05 EU/mL specification (LAL, USP <85>), relevant to TLR4-mediated inflammatory signaling that can confound primary cell phenotype in serum-free conditions.

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HEPES CO2-stable pH

HEPES (30 mM, pKa 7.3 at 37°C) maintains stable pH for sensitive primary cell work without CO2 dependency, useful for open-top chips and atmospheric incubation.

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Customization on demand

pH, glucose, trace element concentrations, HEPES level, and growth factor co-formulations available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes reaching a final 0.04 µm polish, run as two dedicated prefilter-plus-final-filter pairs in series — delivering sub-mycoplasma-scale filtration and particle-free trace element delivery beyond what conventional 0.22 µm filtered MCDB media provide.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates including trace metal salt precipitates; protects the first 0.04 µm final filter from fouling.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains mycoplasma-scale organisms (0.2–0.3 µm) and sub-micron particulates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill and finish.

Performance vs. conventional MCDB media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across four filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma control by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-M201H-B1X MCDB 201 Medium without Sodium Bicarbonate 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MCDB 201 minus Bicarbonate plus HEPES for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) ensuring particle-free trace element delivery.
© Diagnocine® — DCP-M201H-B1X
Applications

Specialized cell models & OoC applications

FluxMPS™ DCP-M201H-B1X was formulated for Chick Embryo Fibroblasts, MSCs and primary fibroblasts under CO2-independent, low-protein or serum-free conditions. The 0.04 µm filtered trace element matrix supports use in microfluidic chip architectures where conventional MCDB media can cause trace metal particulate fouling.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor perfusion, where trace metal salt nanoparticulates below the 0.04 µm cut-off can contribute to valve fouling over long perfusion runs.

  • Trace Metal Nanoparticulate Removal: 0.01 µm filtration targets sub-0.04 µm metal salt colloids not captured by standard QC
  • Valve & Sensor Protection: Reduces micro-fouling risk from trace element aggregates in automated systems
  • Extended Perfusion Stability: Supports consistent trace element delivery over weeks-long primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Organ-on-a-Chip & MPS

Ultra-clean 0.04 µm filtration reduces microchannel fouling risk. Trace elements are delivered particle-free, minimizing aggregate deposition in chip channels.

OoCToCBoCLoCMPS
Fibroblast Biology

Fibroblast-on-Chip

FluxMPS™ MCDB 201 −Bicarbonate +HEPES supports mycoplasma-retentive-filtered, trace-element-complete fibroblast-on-chip models in serum-free or low-protein conditions.

Chick Embryo FibroblastsPrimary Fibroblasts
Stem Cell Biology

MSC OoC

Supports mesenchymal stem cell organ-on-a-chip models requiring stable pH without CO2 control and a particle-free trace element background.

MSCs
Tissue Engineering

Open-Top Connective Tissue Models

CO2-independent HEPES buffering is well suited to open-top chip formats and connective tissue models cultured at ambient atmosphere.

Cell Physiology

CO2-Independent Stromal Culture

30 mM HEPES maintains pH stability for stromal cell culture in incubators or workstations without gas-phase CO2 control.

Metabolic Research

Metalloenzyme & Antioxidant Research

The trace element package (zinc, copper, selenium, manganese) supports studies of metalloenzyme activity, including superoxide dismutase, glutathione peroxidase, and carbonic anhydrase.

SODGPxCarbonic Anhydrase
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 1.441 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 30 mM HEPES, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Trace Elements | [-] Sodium Bicarbonate
Appearance Red-colored, clear solution
Glucose 1441 mg/L (1.441 g/L)
HEPES 30 mM (pKa 7.3 at 37°C)
Trace Elements Present — zinc, copper, selenium, manganese, molybdenum, vanadium, nickel
pH USP <791> 7.4
Osmolality USP <785> 280 - 320 mOsm/kg H2O
Total ingredients 53
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — 30 mM HEPES maintains pH without gas supplementation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MCDB 201 −Bicarbonate +HEPES: 53 ingredients verified per lot with CAS numbers for raw-material traceability. HEPES (30 mM ≈ 7149 mg/L) and D-Glucose are grouped under OTHERS as in the original formulation record.

Component CAS Number mg/L
INORGANIC SALTS
Ammonium metavanadate 7803-55-6 0.000006
Calcium chloride dihydrate 10035-04-8 294.000
Cupric sulfate pentahydrate 7758-99-8 0.00025
Disodium hydrogen phosphate anhydrous 7558-79-4 70.990
Ferrous sulfate heptahydrate 7782-63-0 1.668
Magnesium sulfate anhydrous 7487-88-9 180.570
Manganese sulfate 7785-87-7 0.000075
Molybdic acid ammonium tetrahydrate 12054-85-2 0.000618
Nickel chloride hexahydrate 7791-20-0 0.0000012
Potassium chloride 7447-40-7 223.650
Sodium chloride 7647-14-5 7597.000
Sodium metasilicate nonahydrate 13517-24-3 0.142
Sodium selenite 10102-18-8 0.000865
Zinc sulfate heptahydrate 7446-20-0 0.028744
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.510
L-Alanine 56-41-7 8.910
L-Arginine hydrochloride 1119-34-2 63.200
L-Asparagine monohydrate 5794-13-8 150.000
L-Aspartic acid 56-84-8 13.310
L-Cysteine hydrochloride monohydrate 7048-04-6 35.130
L-Glutamic acid 56-86-0 14.710
L-Glutamine 56-85-9 146.150
L-Histidine hydrochloride monohydrate 5934-29-2 20.970
L-Isoleucine 73-32-5 13.120
L-Leucine 61-90-5 39.350
L-Lysine hydrochloride 657-27-2 36.540
L-Methionine 63-68-3 4.480
L-Phenylalanine 63-91-2 4.960
L-Proline 147-85-3 5.760
L-Serine 56-45-1 31.530
L-Threonine 72-19-5 35.750
L-Tryptophan 73-22-3 6.130
L-Tyrosine disodium salt dihydrate 69847-15-0 11.350
L-Valine 72-18-4 35.130
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 13.960
D-Biotin 58-85-5 0.00733
D-Ca-Pantothenate 137-08-6 0.477
Folinic acid (Calcium)   0.00512
Niacinamide   6.110
Pyridoxine hydrochloride 58-56-0 0.0617
Riboflavin 83-88-5 0.113
Thiamine hydrochloride 67-03-8 0.337
Vitamin B12 68-19-9 0.136
myo-Inositol 87-89-8 18.020
OTHERS
Adenine hydrochloride   1.720
D-Glucose 50-99-7 1441.000
HEPES 7365-45-9 7149.000
Linoleic acid 60-33-3 0.0841
Phenol red sodium salt 34487-61-1 1.242
Putrescine dihydrochloride 333-93-7 0.00161
Sodium pyruvate 113-24-6 55.000
Thioctic acid 1077-28-7 0.00206
Thymidine 50-89-5 0.0727
Custom formulation: Growth factor co-formulations, adjusted trace element concentrations, custom glucose, and pH modifications available. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular attention to trace element dissolution and particulate removal during MCDB formulation.

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ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — used for trace element MCDB formulations where ionic contaminants could otherwise compete with zinc, copper, and selenium uptake.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch production, per-lot traceability, Certificate of Analysis for every lot — trace element concentrations verified against specification.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280 - 320 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-M201H-B1X compares

FluxMPS™ DCP-M201H-B1X vs. conventional 0.22 µm–filtered MCDB 201 −Bicarbonate +HEPES formulations and published classical media specifications.

Parameter DCP-M201H-B1X (FluxMPS™) Conventional MCDB 201 −Bicarbonate +HEPES (0.22 µm filtered) Standard Alt. MCDB (0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
MCDB 201 without Sodium Bicarbonate + HEPES — CO2-independent culture check_circle Yes cancel No cancel No
Trace element delivery Particle-free (0.04 µm) May contain aggregates May contain aggregates
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL
Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-M201H-B1X MCDB 201 −Bicarbonate +HEPES.

Yes. DCP-M201H-B1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels for MPS, OoC, ToC, and LoC platforms. The trace element profile is delivered particle-free — reducing the risk of trace metal aggregate deposition in chip channels — and endotoxin is released to a < 0.05 EU/mL specification, relevant for sensitive primary cell models such as Chick Embryo Fibroblasts.
For MCDB media containing trace elements, this additional filtration also helps ensure trace metal salts remain fully dissolved before filling.
Sodium bicarbonate is removed so that 30 mM HEPES alone provides pH control without CO2. Fibroblasts and MSCs in low-serum or serum-free conditions can be sensitive to pH oscillations during media changes and sampling; HEPES (pKa 7.3 at 37°C) reduces these oscillations in open-top chips and atmospheric incubation environments. If your protocol requires a bicarbonate-buffered, CO2-dependent format instead, contact support@diagnocine.com for a custom formulation.
No. This formulation is CO2-independent — 30 mM HEPES maintains pH without gas-phase CO2 supplementation, making it suitable for open-top chips and atmospheric platforms.
Yes. MCDB media are designed for low-protein or serum-free conditions but can be supplemented with growth factors (EGF, bFGF, VEGF, etc.), dialyzed FBS protein (FBSP, 0.5–2%), antibiotics, or custom nutrients. When adding serum or protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane before combining with the base medium; do not use a 0.04 µm membrane for serum-containing additions, as it will remove large proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulations for your specific cell type.
FluxMPS™ DCP-M201H-B1X is released to a specification of < 0.05 EU/mL, tested by LAL assay per USP <85> on a per-batch basis with an assay sensitivity of 0.005 EU/mL; a Certificate of Analysis is issued for every batch. This matters for primary cell models such as Chick Embryo Fibroblasts and MSCs, where endotoxin can activate TLR4-mediated inflammatory signaling that confounds differentiation and functional readouts independent of experimental treatment.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), the filtration-based mycoplasma control step, and particulate count (USP <788> Method 1), together with raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MCDB 201 −Bicarbonate +HEPES formulations for fibroblast, MSC and organ-on-a-chip applications.

  1. Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-6
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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