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FluxMPS™ MCDB 153 Medium: 1X Liquid

Product#: DCP-M153H1X
$71.49
DCP-M153H1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ MCDB 153 Medium: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate

FluxMPS™ DCP-M153H1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MCDB 153 + HEPES formulation engineered for serum-free or low-protein culture of Human Epidermal Keratinocytes and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final filtration stage delivers the trace element matrix (zinc, copper, selenium, manganese) as fully dissolved ions rather than particulate aggregates, and every manufacturing batch is released to an endotoxin specification of < 0.05 EU/mL. Formulation: [+] 1.081 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 28 mM HEPES, [+] Trace Elements.

  • Trace element formulation — zinc, copper, selenium and manganese for metalloenzyme support and antioxidant activity in serum-free culture
  • Formulated for Human Epidermal Keratinocytes, CHO cells and Chicken Embryo Fibroblasts
  • 28 mM HEPES (pKa 7.3 at 37°C) supplemented with 14 mM sodium bicarbonate — buffering suited to open-top chips and atmospheric platforms
  • 0.04 µm final filtration stage — trace elements delivered as dissolved ions, not particulate aggregates that could foul microfluidic channels
  • Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; Ultrapure Type 1 water (18.2 MΩ·cm) and ISO Class 5 (Class 100) aseptic fill
CAT. NO.
DCP-M153H1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
MCDB 153 Medium: 1X Liquid
  • Cell typesHuman Epidermal Keratinocytes
  • Glucose1081 mg/L (1.081 g/L)
  • HEPES28 mM (pKa 7.3 at 37°C)
  • Sodium Bicarbonate14 mM (1176 mg/L)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

MCDB media formulated for primary cells contain trace element salts that can form subvisible particulate aggregates when filtered at 0.22 µm under typical manufacturing conditions. These aggregates clog microfluidic channels and disrupt trace metal bioavailability. FluxMPS™ addresses these failure modes with a four-stage, sub-0.04 µm filtration train and a batch-release endotoxin specification of < 0.05 EU/mL.

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Particle-free trace element delivery

0.04 µm filtration keeps trace metal salts fully dissolved — reducing the risk of aggregate deposition in chip channels or culture vessels where trace metal precipitation could alter bioavailability.

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Serum-free primary cell support

The trace element composition supports metalloenzyme activity (SOD, GPx, carbonic anhydrase) relevant to primary cell survival and function without serum supplementation.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) limits ionic and organic-carbon contaminants that could compete with trace element uptake by low-serum primary cells.

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Low-endotoxin release specification

Every manufacturing batch is released to an endotoxin specification of < 0.05 EU/mL (LAL, USP <85>), relevant for inflammation-sensitive primary cell models cultured without serum.

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HEPES-stabilized pH

28 mM HEPES (pKa 7.3 at 37°C), supplemented with sodium bicarbonate, supports stable pH for primary cell culture across open and closed chip formats.

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Customization on demand

pH, glucose, trace element concentrations, HEPES, and growth factor co-formulations available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering mycoplasma-retentive filtration and particle-free trace element delivery beyond what conventional 0.22 µm filtered MCDB media can achieve.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, including trace metal salt precipitates, protecting the first 0.04 µm cartridge from fouling.

  2. 2

    0.04 µm Final filtration I

    Mycoplasma-retentive final filter (mycoplasma range 0.2–0.3 µm); retains residual fine particulates not removed by standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge — redundancy, not polishing of the first pass.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of ISO Class 5 aseptic fill & finish.

Performance vs. conventional MCDB media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); 0.1 µm mycoplasma-retentive filtration is applied at every stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-M153H1X MCDB 153 Medium: 1X Liquid, Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I mycoplasma-retentive stage, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish, Microfluidics Suitable MCDB 153 plus HEPES for organ-on-a-chip and microphysiological system applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) supporting particle-free trace element delivery.
© Diagnocine® — DCP-M153H1X
Applications

Specialized cell models & OoC applications

FluxMPS™ DCP-M153H1X was formulated for Human Epidermal Keratinocytes, CHO cells and Chicken Embryo Fibroblasts in low-protein or serum-free conditions. The 0.04 µm filtered trace element matrix supports use in microfluidic chip architectures where conventional MCDB media can cause trace metal particulate fouling.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available for automated bioreactor perfusion, where trace metal salt nanoparticulates in standard MCDB media can cause valve fouling.

  • Trace Metal Nanoparticulate Removal: 10 nm filtration removes sub-0.04 µm metal salt colloids not detected by standard QC
  • Valve & Sensor Protection: Reduces micro-fouling risk from trace element aggregates in automated systems
  • Extended Perfusion Stability: Consistent trace element delivery over weeks-long primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

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Organ-on-a-Chip & MPS

0.04 µm filtration reduces microchannel clogging risk. Trace elements are delivered particle-free, limiting aggregate deposition in chip channels.

Human Epidermal KeratinocytesCHO cellsChicken Embryo Fibroblasts
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Skin-on-Chip

FluxMPS™ MCDB 153 + HEPES supports mycoplasma-retentive filtration and trace element fidelity for skin-on-chip models in serum-free or low-protein conditions.

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Epidermal Barrier OoC

Supports epidermal barrier organ-on-a-chip models requiring particle-free trace element delivery and low-endotoxin culture conditions.

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CHO Bioproduction

Trace element and HEPES-buffered formulation compatible with CHO cell culture workflows requiring particle-free, low-endotoxin media.

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Wound Healing Assays

Serum-free MCDB 153 + HEPES formulation supports keratinocyte and fibroblast wound healing assay models.

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Keratinocyte Differentiation

Trace element and vitamin profile supports keratinocyte differentiation studies in low-protein or serum-free conditions.

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 1.081 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 28 mM HEPES, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Trace Elements
Appearance Red-colored, clear solution
Glucose 1081 mg/L (1.081 g/L)
HEPES 28 mM (6600 mg/L; pKa 7.3 at 37°C)
Sodium Pyruvate 55 mg/L
Trace Elements Present (zinc, copper, selenium, manganese, vanadium, molybdenum, nickel, tin, silicon)
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Total ingredients 55 components across 4 categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement HEPES-buffered (28 mM) with supplemental sodium bicarbonate (14 mM); reduced CO2 dependence for open-top/atmospheric platforms. If a controlled atmosphere is used, approximately 3% CO2 is consistent with the bicarbonate component at pH 7.4
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MCDB 153 + HEPES: 55 ingredients verified per lot with CAS numbers for raw-material traceability. Contains trace elements for metalloenzyme support and antioxidant activity. HEPES (28 mM ≈ 6600 mg/L) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Ammonium metavanadate 7803-55-6 0.000585
Calcium chloride dihydrate 10035-04-8 4.411
Cupric sulfate pentahydrate 7758-99-8 0.00275
Disodium hydrogen phosphate anhydrous 7558-79-4 284.088
Ferrous sulfate heptahydrate 7782-63-0 1.390
Magnesium chloride hexahydrate 7791-18-6 122.000
Manganese sulfate 7785-87-7 0.000151
Molybdic acid ammonium tetrahydrate 12054-85-2 0.00124
Nickel chloride 7718-54-9 0.00012
Potassium chloride 7447-40-7 111.830
Sodium acetate anhydrous 127-09-3 301.530
Sodium bicarbonate 144-55-8 1176.00
Sodium chloride 7647-14-5 7599.000
Stannous chloride monohydrate 10025-69-1 0.000113
Sodium metasilicate nonahydrate 13517-24-3 0.1421
Sodium selenite 10102-18-8 0.0038
Zinc sulfate heptahydrate 7446-20-0 0.144
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.510
L-Alanine 56-41-7 8.910
L-Arginine hydrochloride 1119-34-2 210.700
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 3.990
L-Cysteine hydrochloride monohydrate 7048-04-6 42.040
L-Glutamic acid 56-86-0 14.710
L-Glutamine 56-85-9 877.200
L-Histidine hydrochloride monohydrate 5934-29-2 16.770
L-Isoleucine 73-32-5 1.968
L-Leucine 61-90-5 65.600
L-Lysine hydrochloride 657-27-2 18.270
L-Methionine 63-68-3 4.480
L-Phenylalanine 63-91-2 4.960
L-Proline 147-85-3 34.530
L-Serine 56-45-1 63.060
L-Threonine 72-19-5 11.910
L-Tryptophan 73-22-3 3.060
L-Tyrosine disodium salt dihydrate 69847-15-0 3.410
L-Valine 72-18-4 35.130
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 13.960
D-Biotin 58-85-5 0.0146
D-Pantothenic acid (hemicalcium) 137-08-6 0.238
Folic acid 59-30-3 0.790
Niacinamide 98-92-0 0.03663
Pyridoxine hydrochloride 58-56-0 0.06171
Riboflavin 83-88-5 0.0376
Thiamine hydrochloride 67-03-8 0.337
Vitamin B12 68-19-9 0.407
myo-Inositol 87-89-8 18.020
OTHERS
Adenine hydrochloride 2922-28-3 30.880
D-Glucose 50-99-7 1081.000
HEPES 7365-45-9 6600.000
Phenol red sodium salt 34487-61-1 1.242
Putrescine dihydrochloride 333-93-7 0.161
Sodium pyruvate 113-24-6 55.00
Thioctic acid 1077-28-7 0.206
Thymidine 50-89-5 0.727
Custom formulation: Growth factor co-formulations, adjusted trace element concentrations, custom glucose, and pH modifications available. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular attention to trace element dissolution and particulate removal during MCDB formulation.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at the DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — relevant for trace element MCDB formulations where ionic contaminants could compete with zinc, copper, and selenium uptake.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

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Micro-Batch Precision

Small-batch production, per-lot traceability, and a Certificate of Analysis for every batch — trace element concentrations verified against specification.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310–350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-M153H1X lot at support@diagnocine.com.
Product Comparison

How DCP-M153H1X compares

FluxMPS™ DCP-M153H1X vs. conventional 0.22 µm-filtered MCDB 153 + HEPES formulations.

Parameter DCP-M153H1X (FluxMPS™) Conventional MCDB 153 + HEPES
(0.22 µm filtered)		 Standard Alt. MCDB
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
MCDB 153 (Ham's F-12 modification) with 28 mM HEPES and Trace Elements — for keratinocyte, CHO, and fibroblast OoC check_circle Yes cancel No cancel No
Trace element delivery Particle-free (0.04 µm) May contain aggregates May contain aggregates
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm mycoplasma-retentive) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes (0.04 µm filtered) cancel Higher clogging risk cancel Higher clogging risk
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-M153H1X MCDB 153 + HEPES.

Yes. DCP-M153H1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, supporting low particulate levels for MPS, OoC, ToC, and LoC platforms. The trace element profile is delivered particle-free, reducing the risk of trace metal aggregate deposition in chip channels, and every batch is released to an endotoxin specification of < 0.05 EU/mL.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I (mycoplasma-retentive), 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — Polish — resulting in approximately 5× fewer particles than a single 0.22 µm pass. For MCDB media containing trace elements, this additional filtration also keeps trace metal salts fully dissolved, which limits micro-aggregates that pass through 0.22 µm filters and could otherwise deposit in microfluidic channels.
MCDB 153 is a Ham's F-12 modification with trace elements (zinc, copper, selenium, manganese, and others) and adjusted amino acid/vitamin concentrations optimized for non-transformed cell types in serum-free conditions. These support metalloenzyme activity and cornification/proliferation pathways without serum. For keratinocyte, CHO, or fibroblast applications requiring additional growth factors, contact support@diagnocine.com for a custom co-formulation matched to your cell type.
This formulation is HEPES-buffered (28 mM, pKa 7.3 at 37°C) with supplemental sodium bicarbonate (14 mM), giving reduced CO2 dependence suitable for open-top or atmospheric chip platforms. If a controlled atmosphere is used, approximately 3% CO2 is consistent with the bicarbonate component at the stated pH of 7.4.
Yes. MCDB media are designed for low-protein or serum-free conditions but can be supplemented with growth factors (EGF, bFGF, VEGF, etc.), dialyzed FBS protein (FBSP, 0.5–2%), antibiotics, or custom nutrients. Serum or protein-containing supplements should be pre-filtered through a 0.2 µm, low-protein-binding PES or PVDF membrane before addition — a 0.04 µm membrane is not appropriate for serum-containing solutions. Contact support@diagnocine.com for custom growth factor co-formulations for your specific cell type.
Every manufacturing batch of DCP-M153H1X is tested by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet a release specification of < 0.05 EU/mL before release. This is a batch-level release specification, not a per-unit certificate. Low endotoxin is relevant for primary cell models such as keratinocytes and fibroblasts, where endotoxin-driven inflammatory signaling can confound experimental outcomes.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MCDB 153 + HEPES for Human Epidermal Keratinocyte culture and organ-on-a-chip applications.

  1. Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-6
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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