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FluxMPS™ MCDB 131 Medium: 1X Liquid

Product#: DCP-M1311X
$71.49
DCP-M1311X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ MCDB 131 Medium: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Low Glucose (1000 mg/L) Contains Sodium Pyruvate

FluxMPS™ DCP-M1311X is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MCDB 131 formulation engineered for serum-free or low-protein culture of Human Microvascular Endothelial Cells (HMVEC) and related primary endothelial models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, plus a defined trace element panel (Zn, Cu, Se, Mn, Mo, Ni, V, Fe). Each batch is separately released to meet an endotoxin specification of < 0.05 EU/mL.

  • Formulated for Human Microvascular Endothelial Cells (HMVEC), HUVEC, HAEC and primary endothelial cell culture in serum-free or low-protein conditions
  • Defined trace element panel (Zn, Cu, Se, Mn, Mo, Ni, V, Fe) supports metalloenzyme activity and antioxidant defense without serum supplementation
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) delivers particle-free trace element and salt delivery suited to microfluidic chip channels
  • Low glucose formulation (1000 mg/L) with L-glutamine (1461 mg/L) and sodium pyruvate (110 mg/L) supports endothelial energy metabolism
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ
  • Custom trace element concentrations, glucose levels, pH and growth-factor co-formulations available on request
CAT. NO.
DCP-M1311X | Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
MCDB 131 Medium: 1X Liquid
  • Cell typesHuman Microvascular Endothelial Cells (HMVEC)
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-Glutamine1461 mg/L
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280–320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

MCDB media formulated for primary cells contain trace element salts that can form subvisible particulate aggregates when filtered at 0.22 µm under typical manufacturing conditions. These aggregates clog microfluidic channels, disrupt trace metal bioavailability, and complicate interpretation of sensitive primary cell assays. FluxMPS™ addresses these failure modes with four-stage sub-0.04 µm filtration and a batch-tested < 0.05 EU/mL endotoxin release specification.

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Particle-free trace element delivery

0.04 µm filtration is designed to keep trace metal salts (Zn, Cu, Se, Mn) in solution rather than as aggregates — reducing the risk of deposition in chip channels or culture vessels where precipitation could alter local bioavailability.

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Serum-free primary cell support

The trace element composition supports metalloenzyme activity (SOD, GPx, carbonic anhydrase) relevant to primary cell survival and function in low-serum or serum-free culture.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) limits trace ionic and organic carbon contaminants entering the formulation, relevant where zinc, copper and selenium uptake by low-serum primary cells is being studied.

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Batch-tested endotoxin specification

Each production batch is tested and must meet a release specification of < 0.05 EU/mL, a level designed to stay below thresholds commonly associated with TLR4-mediated inflammatory signaling in vitro — relevant to angiogenesis and barrier-formation assays using HMVEC.

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Rich nutrient profile

The MCDB 131 trace element and amino acid profile supports clonal growth and serum-free survival of microvascular endothelial cells.

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Customization on demand

pH, glucose, trace element concentrations, HEPES, and growth-factor co-formulations available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish — a repeated prefilter-plus-final-filter pair run twice for redundancy — delivering ultra-low particulate counts and particle-free trace element delivery beyond what conventional 0.22 µm filtration provides.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, including trace metal salt precipitates, and protects the downstream 0.04 µm membrane from fouling.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and trace metal salt microaggregates that pass a 0.22 µm filter, and contributes to mycoplasma risk mitigation (mycoplasma, approximately 0.2–0.3 µm).

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge, giving the train full redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill; final mycoplasma-risk-mitigating pass.

Performance vs. conventional MCDB media

5×
Cleaner than 0.22 µm media by particulate count
4
Filtration passes to a 0.04 µm final cut-off
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma risk mitigated by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-M1311X MCDB 131 Medium: 1X Liquid ? Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish ? Microfluidics Suitable MCDB 131 for organ-on-a-chip and HMVEC culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) ensuring particle-free trace element delivery.
© Diagnocine® — DCP-M1311X
Applications

Specialized cell models & OoC applications

FluxMPS™ DCP-M1311X was formulated for Human Microvascular Endothelial Cells (HMVEC), HUVEC, HAEC and primary endothelial cells in low-protein or serum-free conditions. The 0.04 µm filtered trace element matrix is intended to reduce the risk of trace metal particulate fouling in microfluidic chip architectures relative to conventional 0.22 µm filtered MCDB media.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor perfusion applications where sub-0.04 µm trace metal salt colloids in standard MCDB media may contribute to valve fouling.

  • Trace Metal Nanoparticulate Reduction: 10 nm filtration targets sub-0.04 µm metal salt colloids not resolved by standard QC
  • Valve & Sensor Protection: Reduces the risk of micro-fouling from trace element aggregates in automated systems
  • Extended Perfusion Stability: Supports consistent trace element delivery over weeks-long primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

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Organ-on-a-Chip & MPS

Ultra-clean 0.04 µm filtration is designed to reduce microchannel clogging risk. Trace elements are delivered as fully dissolved species, reducing the risk of aggregate deposition in chip channels.

Human Microvascular Endothelial Cells (HMVEC)HUVECHAECprimary endothelial cells
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Vascular Biology

FluxMPS™ MCDB 131 provides sub-mycoplasma-risk filtration and trace element fidelity for vascular biology models in serum-free or low-protein conditions.

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Endothelium-on-Chip

FluxMPS™ MCDB 131 provides sub-mycoplasma-risk filtration and trace element fidelity for endothelium-on-chip models in serum-free or low-protein conditions.

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Angiogenesis Assays

FluxMPS™ MCDB 131 provides sub-mycoplasma-risk filtration and trace element fidelity for angiogenesis assay models in serum-free or low-protein conditions.

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Blood-Brain Barrier OoC

FluxMPS™ MCDB 131 provides sub-mycoplasma-risk filtration and trace element fidelity for blood-brain barrier OoC models in serum-free or low-protein conditions.

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Vascular Permeability

FluxMPS™ MCDB 131 provides sub-mycoplasma-risk filtration and trace element fidelity for vascular permeability models in serum-free or low-protein conditions.

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Available pack sizes: 500 mL, 1000 mL. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Low Glucose (1000 mg/L), [+] Sodium Pyruvate
Appearance Red-colored (phenol red indicator), clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
L-Glutamine 1461 mg/L
Sodium Pyruvate 110 mg/L
Trace Elements Present (Zn, Cu, Se, Mn, Mo, Ni, V, Fe)
pH USP <791> 7.4
Osmolality USP <785> 280–320 mOsm/kg H2O
Total ingredients 53 (4 composition categories: Inorganic Salts, Amino Acids, Vitamins, Others)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Sodium bicarbonate-buffered (14 mM NaHCO3); calculated to support pH 7.4 at approximately 3% CO2 (Henderson-Hasselbalch); validate CO2 set point for your incubator system
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MCDB 131: 53 ingredients across 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others), verified per lot with CAS numbers for raw-material traceability.

Component CAS Number mg/L
INORGANIC SALTS
Ammonium metavanadate 7803-55-6 0.0006
Ammonium molybdate tetrahydrate 12054-85-2 0.0037
Calcium chloride dihydrate 10035-04-8 235.200
Cupric sulfate pentahydrate 7758-99-8 0.0012
Disodium hydrogen phosphate anhydrous 7558-79-4 71.000
Ferrous sulfate heptahydrate 7782-63-0 0.278
Magnesium sulfate anhydrous 7487-88-9 1204.000
Manganese sulfate 7785-87-7 0.00015
Molybdic acid tetrahydrate (ammonium) 12054-85-2 0.0037
Nickel chloride hexahydrate 7791-20-0 0.000071
Potassium chloride 7447-40-7 298.200
Sodium bicarbonate 144-55-8 1180.000
Sodium chloride 7647-14-5 6428.400
Sodium metasillicate nonahydrate 13517-24-3 2.842
Sodium selenite 10102-18-8 0.0052
Zinc sulfate heptahydrate 7446-20-0 0.0003
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 2.250
L-Alanine 56-41-7 2.670
L-Arginine hydrochloride 1119-34-2 63.210
L-Asparagine monohydrate 5794-13-8 15.010
L-Aspartic acid 56-84-8 13.310
L-Cysteine hydrochloride monohydrate 7048-04-6 35.120
L-Glutamic acid 56-86-0 44.130
L-Glutamine 56-85-9 1461.000
L-Histidine hydrochloride monohydratee 5934-29-2 41.920
L-Isoleucine 73-32-5 65.600
L-Leucine 61-90-5 131.200
L-Lysine hydrochloride 657-27-2 182.600
L-Methionine 63-68-3 14.920
L-Phenylalanine 63-91-2 33.040
L-Proline 147-85-3 11.510
L-Serine 56-45-1 31.530
L-Threonine 72-19-5 11.910
L-Tryptophan 73-22-3 4.080
L-Tyrosine disodium salt dihydrate 69847-15-0 22.520
L-Valine 72-18-4 117.100
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 13.960
D-Biotin 58-85-5 0.0073
D-Ca-Pantothenate 137-08-6 11.915
Folinic acid (Calcium) 1492-18-8 0.5115
Niacinamide 98-92-0 6.105
Pyridoxine hydrochloride 58-56-0 2.056
Riboflavin 83-88-5 0.0038
Thiamine hydrochloride 67-03-8 3.373
Vitamin B12 68-19-9 0.0136
myo-Inositol 87-89-8 7.208
OTHERS
Adenine hydrochloride 2922-28-3 1.720
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 12.421
Putrescine dihydrochloride 333-93-7 0.0002
Sodium pyruvate 113-24-6 110.000
Thioctic acid 1077-28-7 0.0021
Thymidine 50-89-5 0.0242
Custom formulation: Growth-factor co-formulations, adjusted trace element concentrations, custom glucose, and pH modifications available. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular attention to trace element dissolution and particulate removal during MCDB formulation.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — used for trace-element MCDB formulations where ionic contaminants may compete with zinc, copper, and selenium uptake.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

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Micro-Batch Precision

Small-batch production, per-lot traceability, Certificate of Analysis for every lot — trace element concentrations verified against specification.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL; assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280–320 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-M1311X compares

FluxMPS™ DCP-M1311X vs. conventional 0.22 µm–filtered MCDB 131 and comparable classical media suppliers.

Parameter DCP-M1311X (FluxMPS™) Conventional / Competitor Products
Grade Microfluidics Suitable Not specified / standard grade
MCDB 131 with defined trace elements — optimized for HMVEC, HUVEC and primary endothelial OoC models check_circle Yes cancel No
Trace element delivery Particle-free (0.04 µm filtered) May contain aggregates
Final filtration pore size 0.04 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stage) cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-M1311X MCDB 131.

DCP-M1311X is processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) delivering ultra-low particulate counts suited to MPS, OoC, ToC, and LoC platforms. Trace elements are delivered as fully dissolved species, reducing the risk of aggregate deposition in chip channels. Separately, each batch is released to meet an endotoxin specification of < 0.05 EU/mL (LAL, USP <85>), relevant for endotoxin-sensitive primary cell models such as HMVEC.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish) — reaching approximately 5× lower particulate counts than 0.22 µm filtered media. For MCDB media containing trace elements, this additional filtration also targets trace metal salt microaggregates that pass through 0.22 µm filters and could otherwise deposit in microfluidic channels or alter trace element bioavailability.
MCDB 131 was originally developed for clonal growth of human microvascular endothelial cells (HMVEC). Trace elements (zinc, copper, selenium, manganese, molybdenum, nickel, vanadium, iron) support endothelial cell metalloenzymes, antioxidant defense (superoxide dismutase), and angiogenic signaling. The 0.04 µm filtration is intended to keep these trace elements in solution, reducing the risk of aggregate deposition in vascular OoC channels.
This formulation contains 1180 mg/L (14 mM) sodium bicarbonate. Based on the Henderson-Hasselbalch equation, this buffering capacity is calculated to support pH 7.4 at approximately 3% CO2; many laboratories run this formulation at 5% CO2 with pH adjusted at time of use. Validate the CO2 set point for your specific incubator and vessel geometry.
Yes. MCDB media are designed for low-protein or serum-free conditions but can be supplemented with growth factors (EGF, bFGF, VEGF, etc.), dialyzed FBS protein (FBSP, 0.5–2%), antibiotics, or custom nutrients. When adding serum or protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — not 0.04 µm, which retains serum proteins and lipoproteins. Contact support@diagnocine.com for custom growth-factor co-formulations for your specific cell type.
FluxMPS™ DCP-M1311X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch rather than per unit. This matters for primary cell models: endotoxin activates TLR4/NF-κB signaling, which can alter angiogenesis (HMVEC), keratinocyte differentiation, fibroblast activation, and glycosylation patterns independently of your experimental design.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), lot number and expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MCDB 131 for Human Microvascular Endothelial Cells (HMVEC) culture and organ-on-a-chip applications.

  1. Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-6
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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