FluxMPS™ MCDB 105 Medium: 1X Liquid

icFluxMPS™ MCDB 105 Medium: 1X Liquid

FluxMPS™ DCP-M105H1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid MCDB 105 formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. It is dual-buffered with sodium bicarbonate (1500 mg/L) plus 25 mM HEPES, and supplied with D-glucose (0.72 g/L), L-glutamine, and sodium pyruvate. Every lot is processed through Diagnocine's four-stage 0.1 µm/0.04 µm filtration train and released against a < 0.05 EU/mL endotoxin specification.

• Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final cut-off
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
• Dual-buffered formulation — sodium bicarbonate (1500 mg/L, ≈17.9 mM) plus 25 mM HEPES for flexibility across CO₂ incubators and open-top chip handling
• D-Glucose 0.72 g/L, L-Glutamine, Sodium Pyruvate, plus the full MCDB 105 trace-element panel (zinc, copper, manganese, molybdenum, selenium, vanadium, tin, nickel)
• 54 verified ingredients per lot across inorganic salts, amino acids, vitamins and other components, each with CAS traceability
• Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
• Historically formulated for long-term survival and rapid clonal growth of human diploid fibroblast-like cell strains (WI-38, MRC-5, IMR-90, HFF)
• Custom pH, salts, HEPES, and nutrient adjustments available on request — contact support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-scale organisms, subvisible particulates, and process residuals that clog microfluidic channels and interfere with sensor readouts. FluxMPS™ is processed through a four-stage 0.1 µm/0.04 µm filtration train to reduce these failure modes, and its dual bicarbonate/HEPES buffer system gives flexibility across CO₂-controlled and open-top chip environments.

Quadruple-stage filtration system

Four serial filtration stages, alternating a 0.1 µm prefilter with a 0.04 µm final filter twice in series, reaching a 0.04 µm final cut-off.

1. 1

   0.1 µm Prefiltration I

   Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; ISO Class 5 aseptic fill & finish.

Designed for next-generation cell models

FluxMPS™ DCP-M105H1X supports demanding platforms from single-channel microfluidic chips to human diploid fibroblast culture. The dual bicarbonate/HEPES buffer system makes it suited to both CO₂-controlled incubators and open-top microfluidic devices.

Automated Bioreactors & Robotics

Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
• Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

54 ingredients verified per lot with CAS numbers for full raw-material traceability. MCDB 105 contains a full trace-element panel (zinc, copper, manganese, selenium, vanadium, tin, nickel, molybdenum) formulated for serum-free diploid fibroblast culture.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-M105H1X compares

FluxMPS™ DCP-M105H1X vs. published supplier specifications for comparable cell culture media.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-M105H1X.

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip, microfluidic, and fibroblast culture research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
4. Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-3
5. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
7. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
8. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b