Long ssDNA Preparation Kit for 10kb
Select LongssDNAPreparationKitfor10kb
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Description:
BioDynamics Laboratory Inc.'s novel Long ssDNA Preparation Kit for 10 kb enables to make 3 to 10 kb of long single strand DNA (LssDNA) by simple method.
Conventional methods for preparing such LssDNA use PCR, exonuclease or reverse trascriptase reaction.
However, LssDNA prepared by these methods may contain mutation or lack inside or end.
Our kit does not use these methods, so LssDNA with precise sequence can be obtained.
Storage Conditions: pLSODN PCR template should be stored at -80℃. Denaturing Gel-Loading Buffer should be stored under -20℃. λ/HindIII DNA for long ssDNA preparation kit should be stored at -80℃. Long ssDNA Gel Extraction kit for 10kb should be stored at 15-25℃, stable for up to 2 years from the date of receipt.
| Image | Cat.No: | Size | Academic/Commercial uses | Link |
 |
FNK-DS635-A | 1 Kit for 10kb | Academic |  |
| FNK-DS635-C | Commercial | |
Features
- LssDNA with precise sequence can be obtained.
- Low contamination of ssDNA without having full length sequence.
- Specially optimized gel extraction kit is included.
Principle
(A) Subcloning objective DNA into plasmid
(B) Digest the vector with nicking enzyme
(C) Do electrophoresis under denaturing condition
(D) Excise gel and purify it with the special column in the kit
Kit Contents
- pLSODN PCR Template 20 μl
- Denaturing Gel-Loading Buffer (#DS611, #DS612) 1 ml
- lambda / Hind III DNA (#DS670) 200 μl (25 ng/μl)
- Long ssDNA Gel Extraction kit for 10kb (#DS650) 25 preps
Attention!
(1) Letter of Assurance (LoA) is required for purchase. Please ask us, support@diagnocine.com(2) For academic or commercial purpose use, license contract should be made between customers and DIagnocine. Please ask us.
(3) Price differs for academia / industrial customers. Please contact us, support@diagnocine.com
