FluxMPS™ Leibovitz's L-15 Medium w/o Sodium Pyruvate, Phenol Red: 2X Liquid
FluxMPS™ Leibovitz's L-15 Medium w/o Sodium Pyruvate, Phenol Red — 2X Liquid
FluxMPS™ DCP-LL15-PR2X is a Microfluidics Suitable, ultra-filtered Leibovitz's L-15 2X formulation engineered for primary cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. CO₂-free by design — this 2X concentrate uses D-galactose and elevated amino acid buffering for atmospheric pH maintenance, with sodium pyruvate and phenol red both omitted for researcher-defined carbon control and autofluorescence-free imaging.
- CO₂-free by design: D-galactose (1.8 g/L) energy source plus elevated amino acid buffering — no gas supplementation required
- 2X concentrate formulated for dilution or direct blending into custom L-15–based protocols
- Sodium pyruvate and phenol red both omitted — enables researcher-defined secondary carbon supplementation and autofluorescence-free live imaging
- Quadruple-stage filtration: 0.1 µm prefiltration I → 0.04 µm final filtration I → 0.1 µm prefiltration II → 0.04 µm final filtration II (polish)
- Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85>)
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- 33 formulation components verified per lot with full CAS raw-material traceability
- Formulation[+] L-Glutamine, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Phenol Red
- Carbon sourceD-Galactose (1.8 g/L) — CO₂-free energy source
- AppearancePale yellow, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)620 – 680 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Sterile, Quadruple-stage)
- CO₂ requirementCO₂-independent by design
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered Leibovitz's L-15 passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that interfere with CO₂-free pH maintenance and live-imaging clarity. FluxMPS™ is engineered against these failure modes.
Microchannel-safe purity
0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate L-15 for clear live imaging and CO₂-free chip loading.
CO₂-free by design formulation
Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and elevated amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation.
Ultrapure-grade water
Type 1 water, 18.2 MΩ·cm — low trace-metal and organic-carbon background supports L-15's amino acid buffering capacity.
Below TLR4 endotoxin threshold
Release specification < 0.05 EU/mL — relevant to minimizing LPS-driven TLR4 activation risk in primary cell culture.
CO₂-free design
D-galactose plus elevated amino acid buffering maintains pH 7.4 in atmospheric conditions — no CO₂ incubator required.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reach a final 0.04 µm polish under aseptic fill conditions.
-
1
0.1 µm Prefiltration I
Large particulate, cell debris and protein aggregate removal; 0.1 µm mycoplasma-retentive filtration (not tested per lot); protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge; redundant mycoplasma-retentive pass.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-LL15-PR2X
CO₂-free live imaging and primary cell applications
FluxMPS™ DCP-LL15-PR2X — Leibovitz's L-15 2X w/o sodium pyruvate, phenol red — delivers 0.04 µm filtered purity for primary cells and related OoC applications.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered variant of this formulation — Diagnocine's separate MPS Grade line — is available on request for automated perfusion systems.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Open-Stage & CO₂-Free Microscopy
L-15 plus galactose maintains pH without CO₂ — a standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning-disk imaging on open-stage microscopes.
Primary Cell Dissociation & Transport
CO₂-free L-15 is a standard dissociation and transport buffer for primary cells, tissue pieces, and organoids outside the incubator.
OoC Loading & Priming
CO₂-independent L-15 enables chip loading, priming, and cell seeding outside incubators without pH drift during critical chip-assembly steps.
In Vivo Imaging & Intravital Microscopy
L-15 is a standard superfusion medium for intravital microscopy, maintaining pH in open tissue preparations without CO₂ during surgical and imaging procedures.
CO₂-Free Metabolic Studies
A galactose-based energy source enables metabolic studies without CO₂ interference; galactose favors oxidative phosphorylation in metabolically flexible cells.
Acute Brain Slice & Neuron Imaging
CO₂-free L-15 supports neuronal viability in acute brain slice preparations and dissociated neuron imaging without requiring carbogen (95% O₂/5% CO₂) gassing.
Analytical release specifications
Every lot released against the full specification matrix. Pack sizes: 500 mL, 1000 mL. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Phenol Red |
| Appearance | Pale yellow, clear solution |
| Carbon source | D-Galactose: 1800 mg/L (1.8 g/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 620 – 680 mOsm/kg H₂O |
| Total ingredients | 33 components across 4 groups |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release — see §Manufacturing) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent by design — Leibovitz's L-15 uses D-galactose and elevated amino acid buffering for CO₂-free pH maintenance. No gas supplementation required. |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Leibovitz's L-15 2X w/o sodium pyruvate, phenol red: 33 ingredients verified per lot with CAS numbers for full raw-material traceability. This formulation uses D-galactose (not glucose) as the primary carbon source and elevated amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 370.000 |
| Magnesium chloride hexahydrate | 7791-18-6 | 400.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 195.440 |
| Potassium chloride | 7447-40-7 | 800.000 |
| Potassium phosphate monobasic | 7778-77-0 | 120.000 |
| Sodium chloride | 7647-14-5 | 16000.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 380.240 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Alanine | 56-41-7 | 450.000 |
| Glycine | 56-40-6 | 400.000 |
| L-Arginine (free base) | 74-79-3 | 1000.000 |
| L-Asparagine | 70-47-3 | 500.000 |
| L-Cysteine (free base) | 52-90-4 | 240.000 |
| L-Glutamine | 56-85-9 | 600.000 |
| L-Histidine (free base) | 71-00-1 | 500.000 |
| L-Isoleucine | 73-32-5 | 500.000 |
| L-Leucine | 61-90-5 | 250.000 |
| L-Lysine hydrochloride | 657-27-2 | 188.000 |
| L-Methionine | 63-68-3 | 150.000 |
| L-Phenylalanine | 63-91-2 | 250.000 |
| L-Serine | 56-45-1 | 400.000 |
| L-Threonine | 72-19-5 | 600.000 |
| L-Tryptophan | 73-22-3 | 40.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 552.320 |
| L-Valine | 72-18-4 | 200.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 2.000 |
| D-Ca-Pantothenate | 137-08-6 | 2.000 |
| Folic acid | 59-30-3 | 2.000 |
| Nicotinamide | 98-92-0 | 2.000 |
| Pyridoxine hydrochloride | 58-56-0 | 2.000 |
| Riboflavin-5-phosphate sodium salt | 130-40-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 2.000 |
| i-Inositol | 87-89-8 | 4.000 |
| OTHERS | ||
| D-Galactose | 59-23-4 | 1800.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — low trace-metal and organic-carbon background.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: 620 – 680 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available on request.
How DCP-LL15-PR2X compares
FluxMPS™ DCP-LL15-PR2X vs. conventional 0.22 µm–filtered Leibovitz's L-15 and standard DMEM/RPMI formulations.
| Parameter | DCP-LL15-PR2X (FluxMPS™) | Conventional Leibovitz's L-15 (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| L-15 2X w/o sodium pyruvate, phenol red — autofluorescence-free with researcher-defined secondary carbon | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle 0.1 µm stage | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-LL15-PR2X — Leibovitz's L-15 2X w/o sodium pyruvate, phenol red.
Supporting literature
Key publications supporting Leibovitz's L-15 2X in CO₂-free live-cell imaging, primary cell culture, and OoC applications.
- Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120329
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Reid Y, et al. Preparation of primary human cells for tissue culture. In: Cells and Culture. Springer; 2009:41–46. doi:10.1007/978-90-481-3419-9_7
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
