FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Phenol Red: 1X Liquid
FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Phenol Red: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). This CO₂-independent, HEPES-buffered, phenol-red-free formulation uses D-galactose (900 mg/L) rather than glucose as its carbon source and is manufactured under ISO 13485:2016 in an ISO Class 5 fill environment.
- CO₂-independent Leibovitz's L-15 base with 25 mM HEPES buffering — no sodium bicarbonate, suitable for ambient-air incubation
- D-Galactose-based carbon source (900 mg/L) in place of glucose, supporting metabolic and Warburg-effect study designs
- L-Glutamine (300 mg/L) and Sodium Pyruvate (550 mg/L) included as ready-to-use energy substrates
- Phenol-red-free formulation — pale/colorless clear solution suited to optical and fluorescence-based assays
- Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) with a 0.04 µm final polish
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ, USA
- Custom pH, HEPES, salts, and nutrient composition available on request — support@diagnocine.com
- Carbon SourceD-Galactose, 900 mg/L (no glucose)
- L-Glutamine300 mg/L ([+])
- Sodium Pyruvate550 mg/L ([+])
- pH (USP <791>)7.4
- Osmolality (USP <785>)300 - 340 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows organisms in the mycoplasma size range (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and confounding metabolic assays. FluxMPS™ closes that gap.
Microchannel-safe purity
A 0.04 µm final filter, tested to USP <788> Method 1 (light obscuration), supports unobstructed flow in channels as narrow as 50 µm.
Total metabolic control
A precisely defined galactose/pyruvate carbon source enables Warburg-effect studies,[3] glycolysis inhibition, and ¹³C metabolic tracing.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) — formulated for low trace-metal and organic-carbon content, supporting consistent lot-to-lot formulation.
Low background for imaging
Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.
Customization on demand
pH, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — two paired prefilter/final-filter passes run in series, each 0.04 µm cartridge protected by its own dedicated 0.1 µm prefilter.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm cartridge and preserves microchannel chip geometries downstream.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter does not.
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3
0.1 µm Prefiltration II
Second, independent prefilter dedicated to protecting the second 0.04 µm cartridge, giving the train full redundancy.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow environment.
Purity architecture, in practice
The quadruple-stage train — two prefilter/final-filter pairs in series — extends particulate and bioburden control well beyond single-pass 0.22 µm filtration, supporting long-duration perfusion in narrow microfluidic channels.
© Diagnocine® — DCP-LL15-R1X
Optimized for next-generation cell biology platforms
FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Phenol Red: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, CO₂-independent culture, and metabolic precision are critical.
Automated Bioreactors & Robotics
For continuous, unattended perfusion systems, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, engineered for automated bioreactors and robotic liquid-handling platforms.
- Total particulate exclusion beyond the 0.04 µm Microfluidics Suitable tier
- Valve and sensor protection in automated dosing systems
- Extended perfusion stability for multi-week unattended runs
Inquiry Required: the 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request this grade.
Micro Physiological System (MPS) & Chip
Ultra-low particulate, HEPES-buffered, CO₂-independent medium for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Galactose-based, glucose-free carbon source enables Warburg-effect studies, aerobic glycolysis suppression, and cancer metabolomics.
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-free perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Bicarbonate-free, phenol-red-free formulation compatible with ¹³C metabolic tracing, Agilent Seahorse XF respiratory assays, and NMR metabolomics.
Microscopy & Optical Sensing
Ultra-low particulate baseline supports confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Full technical specification
Every batch of FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Phenol Red: 1X Liquid is released against comprehensive multi-parameter QC specifications.
Available pack sizes: 500 mL, 1000 mL.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, 25mM HEPES, Calcium, Magnesium, Sodium Pyruvate / [-] Phenol Red |
| Carbon Source | D-Galactose, 900 mg/L (no glucose) |
| Appearance | Clear solution, pale/colorless (phenol-red-free) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 300 - 340 mOsm/kg H2O |
| L-Glutamine | 300 mg/L ([+]) |
| Sodium Pyruvate | 550 mg/L ([+]) |
| Phenol Red | Not added ([-]) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release; see Manufacturing & Compliance) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate USP <788> Method 1 | Light-obscuration particle count compliant |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Grade | Microfluidics Suitable (0.04 µm cut-off) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack (2–8°C) |
| CO₂ requirement | Not required — HEPES-buffered, CO₂-independent formulation (no sodium bicarbonate) |
| Parameter | Specification |
|---|---|
| Raw material grade | Research-grade, per-lot CoA traceable |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 35 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 185.000 |
| Magnesium chloride hexahydrate | 7791-18-6 | 200.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Potassium phosphate monobasic | 7778-77-0 | 60.000 |
| Sodium chloride | 7647-14-5 | 8000.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 190.120 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Alanine | 56-41-7 | 225.000 |
| Glycine | 56-40-6 | 200.000 |
| L-Arginine (free base) | 74-79-3 | 500.000 |
| L-Asparagine | 70-47-3 | 250.000 |
| L-Cysteine (free base) | 52-90-4 | 120.000 |
| L-Glutamine | 56-85-9 | 300.000 |
| L-Histidine (free base) | 71-00-1 | 250.000 |
| L-Isoleucine | 73-32-5 | 250.000 |
| L-Leucine | 61-90-5 | 125.000 |
| L-Lysine hydrochloride | 657-27-2 | 94.000 |
| L-Methionine | 63-68-3 | 75.000 |
| L-Phenylalanine | 63-91-2 | 125.000 |
| L-Serine | 56-45-1 | 200.000 |
| L-Threonine | 72-19-5 | 300.000 |
| L-Tryptophan | 73-22-3 | 20.000 |
| L-Tyrosine disodium salt | 276.160 | |
| L-Valine | 72-18-4 | 100.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin-5-phosphate sodium salt | 130-40-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 2.000 |
| D-Galactose | 59-23-4 | 900.000 |
| HEPES buffer | 7365-45-9 | 5958.000 |
| Sodium pyruvate | 113-24-6 | 550.000 |
Manufacturing & compliance
Every batch is subjected to multi-parameter release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity with low trace-metal and organic-carbon (TOC) content, supporting consistent formulation across lots.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains through final fill.
Micro-Batch Precision
Small-batch production for tight lot-to-lot consistency across pH, osmolality, and endotoxin — critical for reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL, tested per manufacturing batch.
Particulate — USP <788> Method 1
Light-obscuration particle count testing confirms compliance with subvisible particulate limits, supporting clean microchannel flow.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 300 - 340 mOsm/kg H2O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-LL15-R1X compares
Key differences in grade, filtration, mycoplasma control, and endotoxin specification versus conventional 0.22 µm filtered L-15 media.
| Parameter | DCP-LL15-R1X (FluxMPS™) | Standard L-15 (0.22 µm) | Competitor L-15 (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not applicable | Not applicable |
| Formulation definition | [+] L-Glutamine, 25mM HEPES, Calcium, Magnesium, Sodium Pyruvate / [-] Phenol Red | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple: 0.1×2 + 0.04×2) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance (Method 1) | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Phenol Red: 1X Liquid (DCP-LL15-R1X).
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
- Rottem S. Interaction of mycoplasmas with host cells. Physiol Rev. 2003;83:417–432. doi:10.1152/physrev.00030.2002