FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). D-Galactose (0.9 g/L) replaces glucose as the carbon source, consistent with the classic CO₂-independent L-15 design. Manufactured under ISO 13485:2016 using Ultrapure Type 1 water (18.2 MΩ·cm).

• Quadruple-stage filtration to a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2) — Microfluidics Suitable, not an MPS Grade claim
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
• D-Galactose (0.9 g/L) supplied as the carbon source in place of glucose — supports Warburg-effect and CO₂-independent culture models
• L-Glutamine, sodium pyruvate, and phenol red omitted from the base formulation for defined metabolic control and imaging compatibility
• Bicarbonate-free, CO₂-independent formulation — no CO₂ incubator required
• 4× concentrated BME amino acids and vitamins to support extended microfluidic perfusion
• Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
• Custom pH, galactose concentration, salts, and nutrient composition available on request — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

A ready-to-use cell culture medium featuring a validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — capturing sub-micron particulates and mycoplasma-sized organisms that 0.22 µm filtration misses.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter protecting the second 0.04 µm final filter cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in an ISO Class 5 (Class 100) laminar-flow environment.

Optimized for next-generation cell biology platforms

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is suited for applications where microchannel cleanliness, signal fidelity, and CO₂-independent culture are required.

Automated Bioreactors & Robotics

For closed-loop automated bioreactors and robotic perfusion platforms, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation — a distinct six-stage process built on top of this Microfluidics Suitable base, intended for systems where valve and sensor longevity are mission-critical.

• Total Particulate Exclusion — minimizes fouling of micro-valves and inline sensors
• Valve & Sensor Protection — extends service intervals for automated perfusion hardware
• Extended Perfusion Stability — supports uninterrupted multi-week automated culture runs

Inquiry Required: the 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request specifications.

Full technical specification

Every lot of FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is released against multi-parameter QC specifications.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 32 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

How DCP-LL15-QPR1X compares

Differences in filtration depth, endotoxin specification, and formulation control between FluxMPS™ and standard L-15 media.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid (DCP-LL15-QPR1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x