FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 2X Liquid

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 2X Liquid

FluxMPS™ DCP-LL15-QP2X is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Leibovitz's L-15 2X liquid formulation for primary cells, CO₂-free live-cell imaging, and organ-on-a-chip (OoC) / microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. CO₂-free by design — D-galactose plus high amino acid buffering maintains pH without gas supplementation. Formulation: [+] 1.8 g/L D-Galactose | [-] L-Glutamine, [-] Sodium Pyruvate.

• CO₂-free by design: 1.8 g/L D-galactose energy source plus high amino acid buffering — no gas supplementation required
• Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish — approximately 5× lower particulate counts than 0.22 µm–filtered media
• Endotoxin release specification < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch
• Formulated without L-Glutamine and Sodium Pyruvate for independent, application-specific supplementation
• 2X concentrated Leibovitz's L-15 base — dilute or use directly per your protocol
• Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ
• Ultrapure Type 1 water (18.2 MΩ·cm) used throughout processing
• Customization available: pH, nutrient concentrations, HEPES on request — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that interfere with CO₂-free pH maintenance and live-imaging clarity. FluxMPS™ is built to reduce these failure modes.

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under aseptic fill conditions.

1. 1

   0.1 µm Prefiltration I

   Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge. This grade is validated as mycoplasma-retentive.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2's effluent.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter prior to aseptic fill & finish.

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15-QP2X — Leibovitz's L-15 2X — delivers 0.04 µm filtered purity for primary cells and related CO₂-independent applications.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available on request for automated bioreactors and robotic systems requiring the ultra nano-filtered tier.

• Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
• Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

Leibovitz's L-15 2X: 33 ingredients verified per lot with CAS numbers for full raw-material traceability. D-galactose (not glucose) is the primary carbon source, together with high amino acid concentrations, for CO₂-free pH buffering in this 2X concentrated formulation.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

How DCP-LL15-QP2X compares

FluxMPS™ DCP-LL15-QP2X vs. published specifications from other Leibovitz's / classical media suppliers. Where a supplier publishes no figure for a parameter, the cell reads "Not specified" rather than being omitted.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15-QP2X — Leibovitz's L-15 2X.

Supporting literature

Key publications supporting Leibovitz's L-15 2X in CO₂-free live-cell imaging, primary cell culture, and OoC applications.

1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120309
2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Reitzer LJ, Wice BM, Kennell D. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells. J Biol Chem. 1979;254:2669–2676.
6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
7. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
8. Ho WY, Yeap SK, Ho CL, Rahim RA, Alitheen NB. Development of a resazurin-based cytotoxicity assay for cultured primary cells. PLoS One. 2012;7:e44366. doi:10.1371/journal.pone.0044366