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FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Phenol Red: 2X Liquid

Product#: DCP-LL15-QR2X
$99.00
DCP-LL15-QR2X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Phenol Red — 2X Liquid

Contains Calcium Contains Magnesium Contains Sodium Pyruvate Without L-Glutamine Without Phenol Red

FluxMPS™ DCP-LL15-QR2X is a Microfluidics Suitable, quadruple-stage ultra-filtered Leibovitz's L-15 2X formulation built around D-Galactose (1.8 g/L) and sodium pyruvate for CO₂-free pH maintenance — engineered for primary cells and related models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. This formulation omits L-Glutamine (add fresh at time of use) and Phenol Red (reduced background fluorescence contribution from the indicator dye).

  • CO₂-free by design: D-Galactose energy source plus high free amino acid buffering — no gas supplementation required for atmospheric pH maintenance
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish, with 0.1 µm mycoplasma-retentive prefiltration at each pass
  • Endotoxin release specification: < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch
  • Formulated without L-Glutamine and without Phenol Red for fresh nitrogen supplementation at time of use
  • 2X concentrated Leibovitz's L-15 base with 1.8 g/L D-Galactose and 1100 mg/L sodium pyruvate as primary energy sources
  • Manufactured under an ISO 13485:2016 quality management system with per-lot Certificate of Analysis
  • Ultrapure Type 1 water (18.2 MΩ·cm) used throughout formulation and dilution
  • pH, nutrient concentrations, and custom additives available on request — contact support@diagnocine.com
CAT. NO.
DCP-LL15-QR2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 — 2X Liquid
  • Media familyLeibovitz's L-15 2X
  • Carbon (Galactose)1800 mg/L (1.8 g/L, D-Galactose)
  • Formulation[+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red
  • AppearancePale yellow, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)620–680 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
Available sizes: 500 mL, 1000 mL
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's passes mycoplasma-scale particles, subvisible particulates, and endotoxin fragments that interfere with CO₂-free pH stability and live-imaging clarity. FluxMPS™ is engineered against these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance. Reduced particulate load supports clear open-stage live imaging and CO₂-free chip loading.

science

CO₂-free by design formulation

Leibovitz's L-15 uses D-Galactose (not glucose) as the primary carbon source and high concentrations of free amino acids for CO₂-free pH buffering. This is a 2X concentrated formulation intended for dilution or direct 2X use.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) supports trace-metal and organic-carbon (TOC) control during formulation, consistent with the low-endotoxin release specification.

shield

Low endotoxin specification

< 0.05 EU/mL release specification by LAL assay (USP <85>) — well below levels commonly associated with TLR4-mediated activation in endotoxin-sensitive primary cell models.

bolt

CO₂-free design

D-Galactose and high concentrations of free amino acids maintain stable pH in atmospheric conditions — no CO₂ supplementation required.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, alternating 0.1 µm prefiltration with 0.04 µm final filtration, reaching a validated 0.04 µm final pore size.

  1. 1

    0.1 µm Prefiltration I

    Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm cartridge; 0.1 µm is the mycoplasma-retentive filtration grade.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across four filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); 0.1 µm mycoplasma-retentive filtration applied at each pass (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-LL15-QR2X Leibovitz's L-15 2X medium Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip and microfluidic applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15-QR2X
Applications

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15-QR2X — Leibovitz's L-15 2X — delivers 0.04 µm filtered purity for primary cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant of this formulation is available on request for automated perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the MPS Grade 0.01 µm variant.

Live-Cell Imaging

Open-Stage & CO₂-Free Microscopy

L-15 with D-Galactose maintains pH without CO₂ — a standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning-disk imaging on open-stage microscopes.

ConfocalTIRFLight-sheetOpen-stage
Cell Biology

Primary Cell Dissociation & Transport

CO₂-free L-15 is a standard dissociation and transport medium for primary cells, tissue pieces, and organoids outside the incubator.

Primary cellsOrganoidsTissue dissociation
Microfluidics

OoC Loading & Priming

CO₂-independent L-15 enables chip loading, priming, and cell seeding outside incubators without pH drift during chip assembly steps.

OoC loadingChip primingCell seeding
In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is a standard superfusion medium for intravital microscopy, supporting pH stability in open tissue preparations without CO₂ during surgical and imaging procedures.

IntravitalSuperfusionIn vivo
Metabolomics

CO₂-Free Metabolic Studies

The D-Galactose energy source enables metabolic studies without CO₂ interference; galactose forces oxidative phosphorylation in metabolically flexible cells.

OXPHOSGalactose forcingMetabolomics
Neuroscience

Acute Brain Slice & Neuron Imaging

CO₂-free L-15 supports neuronal viability in acute brain slice preparations and dissociated neuron imaging without requiring carbogen (95% O₂/5% CO₂) gassing.

Brain slicesPrimary neuronsNeuronal imaging
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red
Appearance Pale yellow, clear solution
Carbon source D-Galactose: 1800 mg/L (1.8 g/L)
pH USP <791> 7.4
Osmolality USP <785> 620–680 mOsm/kg H₂O
Total ingredients 33
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent by design — D-Galactose and high free amino acid buffering maintain pH without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Leibovitz's L-15 2X: 33 ingredients verified per lot with CAS numbers for raw-material traceability. Leibovitz's L-15 uses D-Galactose (not glucose) as the primary carbon source and high concentrations of free amino acids for CO₂-free pH buffering. This is a 2X concentrated formulation.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 370.000
Magnesium chloride hexahydrate 7791-18-6 400.000
Magnesium sulfate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Potassium phosphate monobasic 7778-77-0 120.000
Sodium chloride 7647-14-5 16000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 380.240
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 450.000
Glycine 56-40-6 400.000
L-Arginine (free base) 74-79-3 1000.000
L-Asparagine 70-47-3 500.000
L-Cysteine (free base) 52-90-4 240.000
L-Histidine (free base) 71-00-1 500.000
L-Isoleucine 73-32-5 500.000
L-Leucine 61-90-5 250.000
L-Lysine hydrochloride 657-27-2 188.000
L-Methionine 63-68-3 150.000
L-Phenylalanine 63-91-2 250.000
L-Serine 56-45-1 400.000
L-Threonine 72-19-5 600.000
L-Tryptophan 73-22-3 40.000
L-Tyrosine disodium salt 69847-45-6 552.320
L-Valine 72-18-4 200.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
i-Inositol 87-89-8 4.000
D-Galactose 59-23-4 1800.000
Sodium pyruvate 113-24-6 1100.000
Custom formulation: Contact support@diagnocine.com for DCP-LL15-QR2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — supports trace-metal and organic-carbon (TOC) control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per manufacturing batch; assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm); light obscuration.

Osmolality — USP <785>

Target: 620–680 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-LL15-QR2X compares

FluxMPS™ DCP-LL15-QR2X vs. conventional 0.22 µm-filtered Leibovitz's and standard DMEM/RPMI formulations.

Parameter DCP-LL15-QR2X (FluxMPS™) Conventional Leibovitz's L-15
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
L-15 2X without L-Glutamine and Phenol Red — CO₂-free base for fresh nitrogen supplementation check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm grade) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
CO₂-free design check_circle Yes (D-Galactose) check_circle Yes cancel No
Microfluidics Suitable channel compatibility check_circle Yes cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15-QR2X — Leibovitz's L-15 2X.

DCP-LL15-QR2X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size (Microfluidics Suitable, 0.04 µm cut-off — a separate 0.01 µm MPS Grade tier is available on request for automated systems). Leibovitz's L-15 CO₂-free formulation makes it well suited for chip loading, priming, open-stage imaging, and atmospheric OoC platforms where CO₂ control is impractical.
 
Phenol red is removed to reduce background fluorescence contribution from the pH indicator dye during live imaging; L-Glutamine is removed because it degrades in liquid storage and is intended to be added fresh at time of use. Add L-Glutamine (or a stable dipeptide alternative) to your working concentration before use.
No. This formulation is CO₂-independent by design — D-Galactose and high concentrations of free amino acids maintain pH in atmospheric conditions. No gas supplementation is required.
Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Serum-containing additions should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm membrane for serum-containing supplements, as it will strip lipoproteins and clog. Contact support@diagnocine.com for custom co-formulation.
FluxMPS™ DCP-LL15-QR2X is produced to a release specification of < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch rather than per unit. This is relevant for primary cell culture, where endotoxin can activate TLR4 signaling and affect cell phenotype.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (LAL assay, USP <85>), 0.1 µm mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X in CO₂-free live-cell imaging, primary cell culture, and OoC applications.

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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