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FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine: 2X Liquid

Product#: DCP-LL15-Q2X
$77.00
DCP-LL15-Q2X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine: 2X Liquid

Contains Phenol Red Contains Calcium Contains Magnesium Contains Sodium Pyruvate Without L-Glutamine

FluxMPS™ DCP-LL15-Q2X is a Microfluidics Suitable, quadruple-stage ultra-filtered Leibovitz's L-15 2X formulation engineered for primary cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. CO₂-free by design — uses D-galactose and high amino acid buffering for atmospheric pH maintenance, in place of sodium bicarbonate.

  • CO₂-free by design: D-galactose energy source + high amino acid buffering — no gas supplementation required
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish for microchannel-safe purity
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85>)
  • Formulated without L-glutamine for fresh addition at time of use, avoiding ammonia accumulation from stored glutamine
  • Contains sodium pyruvate (1100 mg/L) and phenol red (22 mg/L) as a visual pH indicator
  • Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis
  • Ultrapure Type 1 water (18.2 MΩ·cm) processing supports consistent amino acid buffering capacity
CAT. NO.
DCP-LL15-Q2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 2X
  • Media familyLeibovitz's L-15 2X
  • Carbon sourceD-Galactose, 1800 mg/L (1.8 g/L)
  • Formulation[+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] L-Glutamine
  • AppearanceOrange-Red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's L-15 passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that can interfere with CO₂-free pH maintenance and live-cell imaging clarity. FluxMPS™ is engineered to reduce these risks.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> particulate compliance (Method 1, light obscuration) support clear live imaging and reliable CO₂-free chip loading.

science

CO₂-free by design formulation

Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and elevated amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with controlled trace metals and organic carbon (TOC) supports consistent amino acid buffering capacity.

shield

Low endotoxin release specification

< 0.05 EU/mL — formulated to minimize the risk of LPS-driven TLR4 activation in primary cell culture.

bolt

CO₂-free design

D-galactose + high amino acid buffering is intended to maintain pH in atmospheric conditions — no CO₂ incubation required for many applications; validate per protocol.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish under validated aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates — including organisms in the 0.2–0.3 µm mycoplasma size range — that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm final filter cartridge; provides full-redundancy processing.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish under validated ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
4
Filtration passes — 0.1 µm ×2 + 0.04 µm ×2
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-LL15-Q2X Leibovitz's L-15 2X quadruple-stage filtration system, 0.1 micron x2 plus 0.04 micron x2, for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15-Q2X
Applications

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15-Q2X — Leibovitz's L-15 2X — delivers 0.04 µm filtered purity for primary cells and related CO₂-independent applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional MPS Grade 0.01 µm (10 nm) ultra nano-filtered variant (adding 0.02 µm and 0.01 µm stages after the 0.04 µm polish) available on request.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Live-Cell Imaging

Open-Stage & CO₂-Free Microscopy

L-15 + galactose is intended to maintain pH without CO₂ — a common base for prolonged live-cell confocal, TIRF, light-sheet, and spinning-disk imaging on open-stage microscopes.

ConfocalTIRFLight-sheetOpen-stage
Cell Biology

Primary Cell Dissociation & Transport

CO₂-free L-15 is a common dissociation and transport buffer for primary cells, tissue pieces, and organoids outside the incubator.

Primary cellsOrganoidsTissue dissociation
Microfluidics

OoC Loading & Priming

CO₂-independent L-15 supports chip loading, priming, and cell seeding outside incubators, reducing pH drift risk during chip assembly steps.

OoC loadingChip primingCell seeding
In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is used as a superfusion medium for intravital microscopy, supporting pH maintenance in open tissue preparations without CO₂ during surgical and imaging procedures.

IntravitalSuperfusionIn vivo
Metabolomics

CO₂-Free Metabolic Studies

The galactose-based energy source supports metabolic studies without CO₂ interference; galactose is commonly used to force oxidative phosphorylation (OXPHOS) in metabolically flexible cells.

OXPHOSGalactose forcingMetabolomics
Neuroscience

Acute Brain Slice & Neuron Imaging

CO₂-free L-15 is used to support neuronal viability in acute brain slice preparations and dissociated neuron imaging without requiring carbogen (95% O₂/5% CO₂) gassing.

Brain slicesPrimary neuronsNeuronal imaging
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Available pack sizes: 500 mL, 1000 mL. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate | [-] L-Glutamine
Appearance Orange-Red colored, clear solution
Carbon source D-Galactose: 1800 mg/L (1.8 g/L)
L-Glutamine Not added — add fresh at time of use
Sodium Pyruvate 1100 mg/L
Phenol Red 22 mg/L (pH indicator)
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 34
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent by design — Leibovitz's L-15 uses D-galactose and high amino acid buffering for CO₂-free pH maintenance. No gas supplementation required.
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 | Molecular biology and cell culture growth media | (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Leibovitz's L-15 2X: 34 ingredients verified per lot with CAS numbers for full raw-material traceability. Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 370.000
Magnesium chloride hexahydrate 7791-18-6 400.000
Magnesium sulfate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Potassium phosphate monobasic 7778-77-0 120.000
Sodium chloride 7647-14-5 16000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 380.240
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 450.000
Glycine 56-40-6 400.000
L-Arginine (free base) 74-79-3 1000.000
L-Asparagine 70-47-3 500.000
L-Cysteine (free base) 52-90-4 240.000
L-Histidine (free base) 71-00-1 500.000
L-Isoleucine 73-32-5 500.000
L-Leucine 61-90-5 250.000
L-Lysine hydrochloride 657-27-2 188.000
L-Methionine 63-68-3 150.000
L-Phenylalanine 63-91-2 250.000
L-Serine 56-45-1 400.000
L-Threonine 72-19-5 600.000
L-Tryptophan 73-22-3 40.000
L-Tyrosine disodium salt 69847-45-6 552.320
L-Valine 72-18-4 200.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
i-Inositol 87-89-8 4.000
D-Galactose 59-23-4 1800.000
Phenol red sodium salt 34487-61-1 22.000
Sodium pyruvate 113-24-6 1100.000
Custom formulation: Contact support@diagnocine.com for DCP-LL15-Q2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water with controlled trace metals and organic carbon (TOC).

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-LL15-Q2X lot at support@diagnocine.com.
Product Comparison

How DCP-LL15-Q2X compares

FluxMPS™ DCP-LL15-Q2X vs. conventional 0.22 µm–filtered Leibovitz's formulations.

Parameter DCP-LL15-Q2X (FluxMPS™) Conventional Leibovitz's L-15
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
L-15 2X without L-glutamine — fresh nitrogen for CO₂-free primary cell dissociation and transport protocols check_circle Yes cancel No cancel No
CO₂-free design (galactose-based buffering) check_circle Yes check_circle Yes cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm retentive) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Yes, on request cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15-Q2X — Leibovitz's L-15 2X.

DCP-LL15-Q2X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size. Leibovitz's L-15 CO₂-free design makes it suitable for chip loading, priming, open-stage imaging, and atmospheric OoC platforms where CO₂ control is impractical. This product is Microfluidics Suitable (0.04 µm final cut-off), not MPS Grade (0.01 µm); contact us if the 0.01 µm variant is required.
 
L-glutamine is omitted for fresh addition at use (typically 2 mM), preventing degradation and ammonia accumulation from stored glutamine. In L-15's CO₂-free environment used for primary cell dissociation and transport, fresh glutamine helps ensure nitrogen availability throughout the procedure.
CO₂-independent by design — Leibovitz's L-15 uses D-galactose and high amino acid buffering for CO₂-free pH maintenance. No gas supplementation is required; validate pH stability under your own atmospheric conditions.
Yes. FBS (typically 5–10%), serum-free supplements, growth factors, or antibiotics may be added as required. When adding serum or other protein-containing supplements, filter using a 0.2 µm low-protein-binding PES or PVDF membrane rather than a smaller pore size, which can strip serum of active components. Contact support@diagnocine.com for custom co-formulation.
FluxMPS™ DCP-LL15-Q2X is produced to meet a release specification of < 0.05 EU/mL by LAL assay (USP <85>). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification before shipment. For primary cells, low endotoxin helps reduce the risk of TLR4-driven inflammatory activation.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X in CO₂-free live-cell imaging, primary cell culture, and OoC applications.

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120328
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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