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FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-LL15H-PR1X
$55.00
DCP-LL15H-PR1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains HEPES Contains Calcium Contains Magnesium Without Phenol Red Without Sodium Pyruvate

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). This galactose-based, bicarbonate-free formulation is buffered with 25mM HEPES for CO2-independent incubation. Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage filtration to a 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2) for microchannel-safe purity
  • Endotoxin release specification: less than 0.05 EU/mL per manufacturing batch (LAL, USP <85> BET)
  • Galactose-based (0.9 g/L), bicarbonate-free formulation buffered with 25mM HEPES — CO2-independent incubation
  • Formulated without sodium pyruvate and without phenol red for defined, low-background applications
  • Chemically defined amino acid and vitamin profile with micro-batch precision for long-duration MPS perfusion
  • Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
  • Custom pH, HEPES, salts and nutrient adjustments available on request — support@diagnocine.com
DCP-LL15H-PR1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid
  • Galactose0.9 g/L ([+])
  • L-Glutamine300 mg/L ([+])
  • Sodium PyruvateNot added ([-])
  • Phenol RedNot added ([-])
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)300 - 340 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2
  • Storage2-8°C, away from bright light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO

Available pack sizes: 500 mL, 1000 mL. Contact support@diagnocine.com for current pricing.

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

filter_alt

Microchannel-safe purity

0.04 µm final filter and USP <788> Method 1 particulate compliance support unobstructed flow in narrow microfluidic channels.

target

Total metabolic control

A precisely defined galactose-based carbon source enables Warburg-effect studies, glycolysis inhibition, and 13C metabolic tracing.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon control during formulation.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.

science

Rich, stable nutrient profile

Chemically defined amino acid and vitamin profile with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.

tune

Customization on demand

pH, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaches a final pore size of 0.04 µm, run as two dedicated prefilter + final-filter pairs, capturing what 0.22 µm filtration misses entirely.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains fine particulates and sub-micron material entirely absent from standard 0.22 µm media.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm final filter cartridge for full redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Performance vs. conventional media

FluxMPS™ delivers approximately 5× fewer particles ≥10 µm than standard 0.22 µm filtered media — supporting long-duration perfusion without channel occlusion.

0.1µm×2 + 0.04µm×2
Validated four-stage filtration train
0.04
µm final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: 14-day USP <71> sterility tested per batch. The 0.1 µm stages provide mycoplasma-retentive filtration (not tested per lot to USP <63>). Mycoplasma are typically 0.2–0.3 µm in diameter — above the 0.04 µm final cut-off.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid (DCP-LL15H-PR1X) - Quadruple-stage filtration system (0.1 micron x2 + 0.04 micron x2) for organ-on-a-chip and microfluidic MPS cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → ISO Class 5 aseptic fill.
© Diagnocine® — DCP-LL15H-PR1X
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated perfusion systems and robotic liquid handling, Diagnocine offers an optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation.

  • Total Particulate Exclusion — six-stage cascade down to 0.01 µm
  • Valve & Sensor Protection — minimizes fouling in automated flow paths
  • Extended Perfusion Stability — supports unattended, multi-day runs

Inquiry Required: The 0.01 µm MPS Grade variant is available on request — contact support@diagnocine.com.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low particulate, mycoplasma-retentive-filtered media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.

OoCToCBoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Galactose-based, defined carbon source formulation for Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-free perfusion media suitable for TEER measurement, endothelial monolayer integrity, and primary cell culture.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Bicarbonate-free, phenol red-free formulation compatible with 13C metabolic tracing, Agilent Seahorse XF respiratory assays, and NMR metabolomics.

13C tracingSeahorse XFNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate medium for confocal microscopy, fluorescent biosensors, and automated imaging on chip.

ConfocalBiosensorsTEER
Technical Specifications

Full technical specification

Every lot of FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid is released against multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 0.9 g/L Galactose, L-Glutamine, 25mM HEPES / [-] Sodium Pyruvate, Phenol Red
Appearance Clear, colorless solution (phenol red-free)
pH USP <791> 7.4
Osmolality USP <785> 300 - 340 mOsm/kg H2O
Galactose 900 mg/L (0.9 g/L) ([+])
L-Glutamine 300 mg/L ([+])
Sodium Pyruvate Not added ([-])
Phenol Red Not added ([-])
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> < 0.05 EU/mL (batch release spec)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, away from bright light
Freeze–thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2–8°C)
CO2 requirement Not required (CO2-independent; HEPES/phosphate-buffered, bicarbonate-free)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory 21 CFR Part 820 (QMSR) aligned
Production Micro-batch; Totowa, NJ, USA
Intended use RUO only
Formulation

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 34 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Component CAS Number mg/L
Calcium chloride dihydrate 10035-04-8 185.000
Magnesium chloride hexahydrate 7791-18-6 200.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Potassium phosphate monobasic 7778-77-0 60.000
Sodium chloride 7647-14-5 8000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 190.120
Component CAS Number mg/L
L-Alanine 56-41-7 225.000
Glycine 56-40-6 200.000
L-Arginine (free base) 74-79-3 500.000
L-Asparagine 70-47-3 250.000
L-Cysteine (free base) 52-90-4 120.000
L-Glutamine 56-85-9 300.000
L-Histidine (free base) 71-00-1 250.000
L-Isoleucine 73-32-5 250.000
L-Leucine 61-90-5 125.000
L-Lysine hydrochloride 657-27-2 94.000
L-Methionine 63-68-3 75.000
L-Phenylalanine 63-91-2 125.000
L-Serine 56-45-1 200.000
L-Threonine 72-19-5 300.000
L-Tryptophan 73-22-3 20.000
L-Tyrosine disodium salt   276.160
L-Valine 72-18-4 100.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
D-Galactose 59-23-4 900.000
HEPES 7365-45-9 5958.000
i-Inositol 87-89-8 2.000
Custom formulations: pH, galactose, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, low total organic carbon. Supports trace-metal and organic-carbon control for sensitive biosensor and TEER applications.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves filtration gains in the final container.

assignment

Micro-Batch Precision

Small-batch production with tight lot-to-lot consistency in osmolality, pH, and endotoxin — supporting reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL method; assay sensitivity 0.005 EU/mL. Batch release specification: < 0.05 EU/mL.

Filtration — Quadruple-stage

Validated four-stage train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 300 - 340 mOsm/kg H2O.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-LL15H-PR1X compares

Differences in grade, filtration, mycoplasma barrier, and QC depth versus conventional media for MPS and microfluidic applications.

Parameter DCP-LL15H-PR1X (FluxMPS™) Standard L-15 (0.22 µm) Competitor L-15 (0.22 µm)
Grade Microfluidics Suitable Standard grade Standard grade
Formulation [+] 0.9 g/L Galactose, L-Glutamine, 25mM HEPES / [-] Sodium Pyruvate, Phenol Red Standard Standard
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Compliant cancel cancel
Water quality Ultrapure Type 1, 18.2 MΩ·cm Unspecified Unspecified
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle On request cancel cancel

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid (DCP-LL15H-PR1X).

Yes. DCP-LL15H-PR1X is Microfluidics Suitable and engineered for organ-on-a-chip, microfluidic, and MPS applications. The 0.04 µm final filtration removes sub-micron particulates that cause microchannel clogging in standard 0.22 µm media, and the 0.1 µm stages provide a mycoplasma-retentive barrier.
Four sequential stages (0.1 µm ×2 + 0.04 µm ×2) retain mycoplasma-sized organisms (0.2–0.3 µm diameter) and sub-visible particulates entirely missed by conventional 0.22 µm filters — resulting in approximately 5× fewer particles by USP <788> Method 1 light obscuration count.
DCP-LL15H-PR1X combines 0.9 g/L galactose, L-glutamine, and 25mM HEPES with sodium pyruvate and phenol red removed, in an ultra-pure, quadruple-stage filtered base, giving a precisely defined, microchannel-safe starting medium free of a pH indicator dye. Customize pH, nutrients, or buffers at support@diagnocine.com.
No. This formulation contains no sodium bicarbonate; it is buffered by free-base amino acids, phosphate salts, and the 25mM HEPES already included in the formulation, making it suitable for CO2-independent incubation.
Yes. This medium is a basal formulation compatible with FBS (2–10%), human serum, EGF, FGF, VEGF, and antibiotics. Pre-filter serum-containing or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter before combining.
Endotoxin is controlled per manufacturing batch. Every batch is tested by the LAL method (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. Lot-specific results are on the CoA — request at support@diagnocine.com.
Yes — full CoA per lot includes: lot number, expiry, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), and filtration/mycoplasma-barrier confirmation. Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029-1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65-81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118-126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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