FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o Sodium Pyruvate: 2X Liquid

verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15

FluxMPS™ DCP-LL15H-P2X is an MPS-grade, ultra-filtered Leibovitz's L-15 2X + 50mM HEPES formulation engineered for Primary cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. HEPES (50 mM, pKa 7.3 at 37°C) provides robust pH buffering. Formulation: [+] 1.8 g/L D-Galactose, [+] L-Glutamine, [+] 50mM HEPES | [-] Sodium Pyruvate.

CO₂-free by design: D-galactose energy source + high amino acid buffering — no gas supplementation required
50 mM HEPES (pKa 7.3 at 37°C) — robust pH buffering independent of CO₂
0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin
Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill

CAT. NO.

DCP-LL15H-P2X | UNSPSC 41122100 Cell Culture Media

Leibovitz's L-15 — 1X Liquid

Media familyLeibovitz's L-15 2X + 50mM HEPES
Carbon (Galactose)1800 mg/L (1.8 g/L, D-Galactose)
HEPES50 mM, pKa 7.3 at 37°C
Formulation[+] 1.8 g/L D-Galactose, [+] L-Glutamine, [+] 50mM HEPES | [-] Sodium Pyruvate
AppearanceOrange-Red colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)Contact for specification
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Shelf Life12 months / 2–8°C

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's passes mycoplasma, subvisible particulates, and endotoxin fragments that interfere with CO₂-free pH maintenance and live imaging clarity. FluxMPS™ eliminates these failure modes.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particle-free L-15 for clear live imaging and CO2-free chip loading.

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CO₂-free by design formulation

Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation. 50 mM HEPES provides e

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Ultrapure-grade water

18.2 MΩ·cm — eliminates ionic contaminants that interfere with L-15 amino acid buffering capacity.

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Below TLR4 endotoxin threshold

< 0.05 EU/mL — prevents LPS-driven inflammatory activation in primary cells.

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HEPES pH stability

HEPES (50 mM) prevents pH rise during open-air handling, flow cytometry preparation, and atmospheric incubation.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1

0.1 µm Pre-filtration I — Large Particulate Removal

Removes large aggregates; protects downstream 0.04 µm membranes.
2

0.04 µm Pre-filtration II — Mycoplasma Barrier

Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration.
3

0.1 µm Sterile-filtration I — Second-pass Redundancy

Second-pass sterility redundancy; no breakthrough from Stage 1.
4

0.04 µm Sterile-filtration II — Final Polish

Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-LL15H-P2X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered Leibovitz's, with confirmed mycoplasma barrier at every production stage.

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size — sub-mycoplasma polishing

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier every lot.

FluxMPS-Quadruple-stage-filtration-system-Cell-Culture-Media-Diagnocine-V2.jpg — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15H-P2X

Applications

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15H-P2X — Leibovitz's L-15 2X + 50mM HEPES — delivers 0.04 µm filtered purity for Primary cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered variant available on request.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Live-Cell Imaging

Open-Stage & CO₂-Free Microscopy

L-15 + galactose maintains pH without CO₂ — the standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning disk imaging on open-stage microscopes.

ConfocalTIRFLight-sheetOpen-stage

Cell Biology

Primary Cell Dissociation & Transport

CO₂-free L-15 is the standard dissociation and transport buffer for primary cells, tissue pieces, and organoids outside the incubator.

Primary cellsOrganoidsTissue dissociation

Microfluidics

OoC Loading & Priming

CO₂-independent L-15 enables chip loading, priming, and cell seeding outside incubators without pH drift during the critical chip assembly steps.

OoC loadingChip primingCell seeding

In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is the standard superfusion medium for intravital microscopy, maintaining pH in open tissue preparations without CO₂ during surgical and imaging procedures.

IntravitalSuperfusionIn vivo

Metabolomics

CO₂-Free Metabolic Studies

Galactose-based energy source enables metabolic studies without CO₂ interference. Galactose forces OXPHOS in metabolically flexible cells.

OXPHOSGalactose forcingMetabolomics

Neuroscience

Acute Brain Slice & Neuron Imaging

CO₂-free L-15 maintains neuronal viability in acute brain slice preparations and dissociated neuron imaging without requiring carbogen (95% O₂/5% CO₂) gassing.

Brain slicesPrimary neuronsNeuronal imaging

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] 1.8 g/L D-Galactose, [+] L-Glutamine, [+] 50mM HEPES \| [-] Sodium Pyruvate
Appearance	Orange-Red colored, clear solution
Carbon source	D-Galactose: 1800 mg/L (1.8 g/L, D-Galactose)
HEPES	50 mM (pKa 7.3 at 37°C)
pH USP <791>	7.4
Osmolality USP <785>	Contact for specification
Total ingredients	35

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788>	NMT 25/mL
Particulate ≥25 µm USP <788>	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from manufacture
Shipping condition	cold pack
CO₂ requirement	CO₂-independent by design — Leibovitz's L-15 uses galactose and high amino acid buffering for CO₂-free pH maintenance. No gas supplementation required.

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
Regulatory alignment	21 CFR Part 820 (cGMP) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

Leibovitz's L-15 2X + 50mM HEPES: 35 ingredients verified per lot with CAS numbers for full raw-material traceability. Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation. 50 mM HEPES provides enhanced pH buffering for prolonged open-stage procedures.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	370.000
Magnesium chloride hexahydrate	7791-18-6	400.000
Magnesium sulphate anhydrous	7487-88-9	195.440
Potassium chloride	7447-40-7	800.000
Potassium phosphate monobasic	7778-77-0	120.000
Sodium chloride	7647-14-5	16000.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	380.240

Component	CAS Number	mg/L
AMINO ACIDS
L-Alanine	56-41-7	450.000
Glycine	56-40-6	400.000
L-Arginine (free base)	74-79-3	1000.000
L-Asparagine	70-47-3	500.000
L-Cysteine (free base)	52-90-4	240.000
L-Glutamine	56-85-9	600.000
L-Histidine (free base)	71-00-1	500.000
L-Isoleucine	73-32-5	500.000
L-Leucine	61-90-5	250.000
L-Lysine hydrochloride	657-27-2	188.000
L-Methionine	63-68-3	150.000
L-Phenylalanine	63-91-2	250.000
L-Serine	56-45-1	400.000
L-Threonine	72-19-5	600.000
L-Tryptophan	73-22-3	40.000
L-Tyrosine disodium salt	69847-45-6	552.320
L-Valine	72-18-4	200.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	2.000
D-Ca-Pantothenate	137-08-6	2.000
Folic acid	59-30-3	2.000
Nicotinamide	98-92-0	2.000
Pyridoxine hydrochloride	58-56-0	2.000
Riboflavin-5-phosphate sodium salt	130-40-5	0.200
Thiamine hydrochloride	67-03-8	2.000
i-Inositol	87-89-8	4.000
OTHERS
D-Galactose	59-23-4	1800.000
HEPES buffer	7365-45-9	11915.000
Phenol red sodium salt	34487-61-1	22.000

Custom formulation: Contact support@diagnocine.com for DCP-LL15H-P2X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL per lot.

Particulate — USP <788> Method 2

≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Certificate of Analysis: Request for any DCP-LL15H-P2X lot at support@diagnocine.com.

Product Comparison

How DCP-LL15H-P2X compares

FluxMPS™ DCP-LL15H-P2X vs. conventional 0.22 µm–filtered Leibovitz's formulations.

Parameter	DCP-LL15H-P2X (FluxMPS™)	Conventional Leibovitz's L-1 (0.22 µm filtered)	Standard DMEM/RPMI (0.22 µm filtered)
L-15 2X + 50mM HEPES — no Pyruvate; researcher-defined secondary carbon with enhanced pH buffering	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier	check_circle Yes (0.04 µm)	cancel No	cancel No
CO₂-free design	check_circle Yes (galactose)	check_circle Yes	cancel No
HEPES (50 mM)	check_circle Yes	cancel Usually no	cancel No
Endotoxin specification	< 0.05 EU/mL	NMT 1 EU/mL	NMT 1 EU/mL
USP <788> particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle MPS-grade	cancel Risk of clogging	cancel Risk of clogging

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15H-P2X — Leibovitz's L-15 2X + 50mM HEPES.

DCP-LL15H-P2X is processed through a Quadruple-stage filtration system reaching 0.04 µm final pore size. Leibovitz L-15 CO₂-free design makes it ideal for chip loading, priming, open-stage imaging, and atmospheric OoC platforms where CO₂ control is impractical.

FluxMPS™ uses four sequential filters — 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish — resulting in approximately 5× fewer particles and confirmed mycoplasma retention at every production stage.

Pyruvate omitted for researcher-defined fresh addition. 50 mM HEPES provides enhanced pH stability during the supplementation step and throughout prolonged CO₂-free live imaging.

CO₂-independent by design — Leibovitz's L-15 uses galactose and high amino acid buffering for CO₂-free pH maintenance. No gas supplementation required..

Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Contact support@diagnocine.com for custom co-formulation.

FluxMPS™ DCP-LL15H-P2X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>). For primary cells, endotoxin activates TLR4 altering cell phenotype and differentiation outcomes.

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X + 50mM HEPES in CO₂-free live-cell imaging, primary cell culture, and OoC applications.

Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1083/jcb.1.3.273
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o Sodium Pyruvate: 2X Liquid