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FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o Sodium Pyruvate: 2X Liquid

Product#: DCP-LL15H-P2X
$88.00
DCP-LL15H-P2X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o Sodium Pyruvate — 2X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES (50 mM) Contains Calcium Contains Magnesium Without Sodium Pyruvate

FluxMPS™ DCP-LL15H-P2X is a Microfluidics Suitable, ultra-filtered Leibovitz's L-15 2X + 50mM HEPES formulation engineered for primary cells and related models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Built around a D-galactose carbon source with 50 mM HEPES (pKa 7.3 at 37°C), it maintains pH without CO2 gas supplementation.

  • CO2-independent by design: 1.8 g/L D-galactose plus elevated amino acid buffering maintain pH without gas supplementation
  • 50 mM HEPES (pKa 7.3 at 37°C) provides robust, open-air pH stability for prolonged live imaging and chip loading
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>) on every manufacturing batch
  • Formulated without sodium pyruvate, allowing researcher-defined supplementation at time of use
  • 2X concentrated Leibovitz's L-15 base — dilute to working strength per protocol
  • Manufactured under an ISO 13485:2016 quality management system with per-lot Certificate of Analysis
  • Engineered for microfluidic channels, organ-on-a-chip (OoC) and open-stage live-cell imaging platforms
CAT. NO.
DCP-LL15H-P2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 Medium with 50mM HEPES, w/o Sodium Pyruvate — 2X Liquid
  • Media familyLeibovitz's L-15 2X + 50mM HEPES
  • Carbon source (Galactose)1800 mg/L (1.8 g/L, D-Galactose)
  • HEPES50 mM, pKa 7.3 at 37°C
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] HEPES (50 mM), [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate
  • AppearanceOrange-Red colored, clear solution
  • pH (USP <791>)7.4
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's passes mycoplasma-scale contaminants and subvisible particulates that interfere with open-air pH maintenance and live-imaging clarity. FluxMPS™ is built to reduce these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> particulate testing keep subvisible particulates and mycoplasma-scale debris out of narrow microfluidic channels and open-stage imaging fields.

target

CO2-independent metabolic control

Leibovitz's L-15 replaces glucose with D-galactose (1.8 g/L) and relies on elevated amino acid buffering, giving researchers a defined carbon source and CO2-free pH conditions outside the incubator.

water_drop

Ultrapure-grade water

Type 1 water at 18.2 MΩ·cm minimizes trace-metal and organic-carbon contamination introduced during manufacture.

visibility

Low background for imaging

Quadruple-stage filtration reduces particulate baseline for confocal, TIRF, and light-sheet imaging on open-stage microscopes. Formulation includes phenol red as a visual pH indicator.

science

Rich, stable nutrient profile

Full complement of amino acids and B-vitamins at Leibovitz's L-15 2X concentration, supplemented with 50 mM HEPES for extended buffering during prolonged open-air procedures.

tune

Customization on demand

pH, HEPES concentration, salts, and nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; provides mycoplasma-retentive filtration (mycoplasma range 0.2–0.3 µm), a stage absent from standard 0.22 µm–filtered media.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge for the redundant final pass.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-LL15H-P2X Leibovitz's L-15 2X liquid medium with 50mM HEPES, Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2, for organ-on-a-chip and microfluidic cell culture applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15H-P2X
Applications

CO2-free live imaging and primary cell applications

FluxMPS™ DCP-LL15H-P2X — Leibovitz's L-15 2X + 50mM HEPES — delivers 0.04 µm filtered purity for primary cells and related open-air, microfluidic and OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional MPS Grade 0.01 µm (10 nm) ultra nano-filtered variant of this formulation is available on request for automated perfusion and robotic handling systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Live-Cell Imaging

Open-Stage & CO2-Free Microscopy

L-15 with D-galactose maintains pH without CO2 — a standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning disk imaging on open-stage microscopes.

ConfocalTIRFLight-sheetOpen-stage
Cell Biology

Primary Cell Dissociation & Transport

CO2-free L-15 is a standard dissociation and transport buffer for primary cells, tissue pieces, and organoids handled outside the incubator.

Primary cellsOrganoidsTissue dissociation
Microfluidics

OoC Loading & Priming

CO2-independent L-15 enables chip loading, priming, and cell seeding outside incubators without pH drift during chip assembly.

OoC loadingChip primingCell seeding
In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is used as a superfusion medium for intravital microscopy, helping maintain pH in open tissue preparations without CO2 during surgical and imaging procedures.

IntravitalSuperfusionIn vivo
Metabolomics

CO2-Free Metabolic Studies

D-galactose as the energy source enables metabolic studies without CO2 interference, and forces oxidative phosphorylation in metabolically flexible cells.

OXPHOSGalactose forcingMetabolomics
Neuroscience

Acute Brain Slice & Neuron Imaging

CO2-free L-15 supports neuronal viability in acute brain slice preparations and dissociated neuron imaging without requiring carbogen (95% O2/5% CO2) gassing.

Brain slicesPrimary neuronsNeuronal imaging
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] HEPES (50 mM), [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate
Appearance Orange-Red colored, clear solution
Carbon source D-Galactose: 1800 mg/L (1.8 g/L)
HEPES 50 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 35 components across 3 formulation groups
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> M1 NMT 25/mL
Particulate ≥25 µm USP <788> M1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent by design — galactose and high amino acid buffering maintain pH without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Available pack sizes 500 mL, 1000 mL
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Leibovitz's L-15 2X + 50mM HEPES: 35 ingredients verified per lot with CAS numbers for full raw-material traceability. This 2X concentrated formulation uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO2-free pH buffering; 50 mM HEPES provides enhanced pH buffering for prolonged open-stage procedures.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 370.000
Magnesium chloride hexahydrate 7791-18-6 400.000
Magnesium sulfate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Potassium phosphate monobasic 7778-77-0 120.000
Sodium chloride 7647-14-5 16000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 380.240
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 450.000
Glycine 56-40-6 400.000
L-Arginine (free base) 74-79-3 1000.000
L-Asparagine 70-47-3 500.000
L-Cysteine (free base) 52-90-4 240.000
L-Glutamine 56-85-9 600.000
L-Histidine (free base) 71-00-1 500.000
L-Isoleucine 73-32-5 500.000
L-Leucine 61-90-5 250.000
L-Lysine hydrochloride 657-27-2 188.000
L-Methionine 63-68-3 150.000
L-Phenylalanine 63-91-2 250.000
L-Serine 56-45-1 400.000
L-Threonine 72-19-5 600.000
L-Tryptophan 73-22-3 40.000
L-Tyrosine disodium salt 69847-45-6 552.320
L-Valine 72-18-4 200.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
i-Inositol 87-89-8 4.000
D-Galactose 59-23-4 1800.000
HEPES buffer 7365-45-9 11915.000
Phenol red sodium salt 34487-61-1 22.000
Custom formulation: Contact support@diagnocine.com for DCP-LL15H-P2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — minimizes trace-metal and organic-carbon contamination during manufacture.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per manufacturing batch (assay sensitivity 0.005 EU/mL).

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm) by light obscuration particle count.

Osmolality — USP <785>

Target: Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request for any DCP-LL15H-P2X lot at support@diagnocine.com.
Product Comparison

How DCP-LL15H-P2X compares

FluxMPS™ DCP-LL15H-P2X vs. conventional 0.22 µm–filtered Leibovitz's and standard 0.22 µm–filtered DMEM/RPMI formulations.

Parameter DCP-LL15H-P2X (FluxMPS™) Conventional Leibovitz's L-15 (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Not applicable Not applicable
Formulation match (L-15 2X + 50mM HEPES, no pyruvate) check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stage) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate testing (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
CO2-independent design check_circle Yes (galactose + HEPES) check_circle Yes cancel No
Microfluidic channel compatibility check_circle Yes (Microfluidics Suitable, 0.04 µm) cancel Higher clogging risk cancel Higher clogging risk
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15H-P2X — Leibovitz's L-15 2X + 50mM HEPES.

DCP-LL15H-P2X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size. Leibovitz's L-15's CO2-independent design makes it well suited for chip loading, priming, open-stage imaging, and atmospheric OoC platforms where CO2 control is impractical.
 
Sodium pyruvate is omitted so researchers can add it fresh at time of use if their cell model requires it. 50 mM HEPES provides enhanced pH stability during the supplementation step and throughout prolonged CO2-free live imaging.
No. This formulation is CO2-independent by design — Leibovitz's L-15 uses D-galactose and high amino acid buffering for CO2-free pH maintenance, so no gas supplementation is required for open-air handling.
Yes. FBS (5–10%), serum-free supplements, growth factors, or antibiotics may be added. When adding serum or other protein-containing supplements, pre-filter through a 0.2 µm low-protein-binding PES or PVDF membrane immediately before use — never a 0.04 µm membrane, which will strip serum of essential high-molecular-weight components. Contact support@diagnocine.com for custom co-formulation.
DCP-LL15H-P2X is produced to a release specification of < 0.05 EU/mL, verified by LAL assay (USP <85>) on every manufacturing batch. Endotoxin is controlled per batch rather than per unit; a Certificate of Analysis is available for each lot on request.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X + 50mM HEPES in CO2-free live-cell imaging, primary cell culture, and OoC applications.

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. PMID: 14043330
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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