FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate: 1X Liquid
FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Buffered with 25 mM HEPES and formulated without sodium pyruvate, this bicarbonate-free, CO₂-independent medium is manufactured under an ISO 13485:2016 quality system using a validated four-stage 0.1 µm/0.04 µm filtration train.
- Leibovitz's L-15 formulation buffered with 25 mM HEPES; bicarbonate-free and CO₂-independent, suited to open-bench and microfluidic culture
- 0.9 g/L D-Galactose provided as the carbon source in place of glucose, consistent with the classical L-15 design for ambient-air culture
- L-Glutamine included at 300 mg/L ([+]); sodium pyruvate not added ([-]) for defined metabolic control
- Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) validated for microfluidic channel compatibility
- Endotoxin release specification: < 0.05 EU/mL (USP <85> BET), controlled per manufacturing batch
- Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
- Custom pH, HEPES, salts, and nutrient adjustments available on request — support@diagnocine.com
- Galactose0.9 g/L (Present)
- L-Glutamine300 mg/L ([+])
- Sodium PyruvateNot added ([-])
- pH (USP <791>)7.4
- Osmolality (USP <785>)300 - 340 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap with a validated 0.04 µm final polish.
Microchannel-safe purity
A 0.04 µm final filter provides a substantially finer cut-off than conventional single-stage 0.22 µm filtration, supporting more consistent flow through narrow microfluidic geometries.
Total metabolic control
Galactose in place of glucose enables defined-carbon-source studies, Warburg-effect research, and ¹³C metabolic tracing on a precisely specified baseline.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) with tight trace-metal and organic-carbon control, supporting sensitive biosensor and electrochemical chip applications.
Low background for imaging
Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.
Customization on demand
pH, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate is manufactured with a validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — two dedicated prefilter/final-filter pairs run in series for redundant clearance.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter cartridge.
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2
0.04 µm Final filtration I
Retains sub-micron particulates and microaggregates that pass through 0.22 µm filtration unimpeded.
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3
0.1 µm Prefiltration II
A second dedicated prefilter, protecting the second 0.04 µm final filter cartridge for redundant clearance.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Filtration architecture
Two dedicated 0.1 µm/0.04 µm prefilter and final-filter pairs run in series, each 0.04 µm final filter protected by its own 0.1 µm prefilter, for a validated final cut-off well below conventional 0.22 µm filtration.
© Diagnocine® — DCP-LL15H-P1X
Optimized for next-generation cell biology platforms
FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and CO₂-independent culture stability are critical.
Automated Bioreactors & Robotics
For automated bioreactor and robotic liquid-handling platforms requiring the finest available cut-off, an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request.
- Total Particulate Exclusion — six-stage cascade down to 0.01 µm
- Valve & Sensor Protection — reduced risk of micro-valve and sensor fouling in closed-loop systems
- Extended Perfusion Stability — supports longer unattended perfusion intervals
Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com for lead time and pricing.
Micro Physiological System (MPS) & Chip
Ultra-low particulate media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices, in an ambient-air, CO₂-independent format.
Warburg Effect & Metabolic Research
Galactose-based carbon source formulation supports Warburg-effect studies, aerobic glycolysis research, and cancer metabolomics.
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-free, CO₂-independent perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture on the open bench.
Metabolic Flux Analysis
Chemically defined, galactose-based formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium.
Microscopy & Optical Sensing
Low particulate baseline for confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Full technical specification
Every lot of FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate: 1X Liquid is released against comprehensive multi-parameter QC specifications. Available pack sizes: 500 mL, 1000 mL.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, Phenol Red, HEPES, Calcium, Magnesium / [-] Sodium Pyruvate |
| Appearance | Red to pink-red, clear solution (phenol red pH indicator present) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 300 - 340 mOsm/kg H₂O |
| Glucose | Not added; D-Galactose (0.9 g/L) provided as carbon source |
| L-Glutamine | 300 mg/L ([+]) |
| Sodium Pyruvate | Not added ([-]) |
| Phenol Red | 11 mg/L ([+]) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm <788> Method 1 | Meets acceptance criteria |
| Particulate ≥25 µm <788> Method 1 | Meets acceptance criteria |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO₂ requirement | Not required (CO₂-independent; HEPES-buffered, bicarbonate-free) |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 35 components. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| Calcium chloride dihydrate | 10035-04-8 | 185.000 |
| Magnesium chloride hexahydrate | 7791-18-6 | 200.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Potassium phosphate monobasic | 7778-77-0 | 60.000 |
| Sodium chloride | 7647-14-5 | 8000.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 190.120 |
| Component | CAS Number | mg/L |
|---|---|---|
| L-Alanine | 56-41-7 | 225.000 |
| Glycine | 56-40-6 | 200.000 |
| L-Arginine (free base) | 74-79-3 | 500.000 |
| L-Asparagine | 70-47-3 | 250.000 |
| L-Cysteine (free base) | 52-90-4 | 120.000 |
| L-Glutamine | 56-85-9 | 300.000 |
| L-Histidine (free base) | 71-00-1 | 250.000 |
| L-Isoleucine | 73-32-5 | 250.000 |
| L-Leucine | 61-90-5 | 125.000 |
| L-Lysine hydrochloride | 657-27-2 | 94.000 |
| L-Methionine | 63-68-3 | 75.000 |
| L-Phenylalanine | 63-91-2 | 125.000 |
| L-Serine | 56-45-1 | 200.000 |
| L-Threonine | 72-19-5 | 300.000 |
| L-Tryptophan | 73-22-3 | 20.000 |
| L-Tyrosine disodium salt | 276.160 | |
| L-Valine | 72-18-4 | 100.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin-5-phosphate sodium salt | 130-40-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 2.000 |
| D-Galactose | 59-23-4 | 900.000 |
| HEPES buffer | 7365-45-9 | 5958.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
Manufacturing & compliance
Every batch is subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at DiagnoCine R&D Center; customization at DiagnoCine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, low trace-metal and organic-carbon content, USP <85> endotoxin-tested.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves all filtration gains in the final container.
Micro-Batch Precision
Small-batch production with tight lot-to-lot consistency — important for reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light-obscuration particle count test. Each lot meets Method 1 acceptance criteria.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 300 - 340 mOsm/kg H₂O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-LL15H-P1X compares
Filtration architecture, mycoplasma-barrier design, water quality, and QC depth relative to conventionally filtered L-15 media.
| Parameter | DCP-LL15H-P1X (FluxMPS™) | Standard L-15 (0.22 µm) | Competitor L-15 (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Formulation definition | [+] L-Glutamine, Phenol Red, HEPES, Calcium, Magnesium / [-] Sodium Pyruvate | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Not specified | Not specified |
| Manufacturing QMS | ISO 13485:2016 | Not specified | Not specified |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o Sodium Pyruvate: 1X Liquid (DCP-LL15H-P1X).
Supporting literature
- Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120328
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7