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FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o Phenol Red: 2X Liquid

Product#: DCP-LL15H-R2X
$99.00
DCP-LL15H-R2X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o Phenol Red — 2X Liquid

Contains L-Glutamine Contains HEPES (50 mM) Contains Calcium Contains Magnesium Contains Sodium Pyruvate Without Phenol Red

FluxMPS™ DCP-LL15H-R2X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Leibovitz's L-15 2X formulation with 50 mM HEPES, engineered for primary cells and related models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. D-Galactose (1.8 g/L) replaces glucose as the carbon source, and high amino acid concentrations plus 50 mM HEPES (pKa 7.3 at 37°C) maintain pH without CO₂ supplementation. Phenol red is excluded to support autofluorescence-free live-cell imaging.

  • CO₂-free by design: D-galactose (1.8 g/L) energy source plus high amino acid buffering — no gas supplementation required for pH maintenance
  • 50 mM HEPES (pKa 7.3 at 37°C) provides robust, CO₂-independent pH buffering for prolonged open-stage procedures
  • Phenol red-free formulation supports autofluorescence-free live-cell confocal, TIRF, and light-sheet imaging
  • A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
  • Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>)
  • Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine's R&D Center, Totowa, NJ
  • 2X concentrated Leibovitz's L-15 formulation with L-glutamine (600 mg/L) and sodium pyruvate (1100 mg/L)
CAT. NO.
DCP-LL15H-R2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 — 2X Liquid, 50 mM HEPES, w/o Phenol Red
  • Carbon source (D-Galactose)1800 mg/L (1.8 g/L)
  • L-Glutamine600 mg/L
  • Sodium Pyruvate1100 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)620–680 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's L-15 passes mycoplasma-sized organisms and subvisible particulates that interfere with open-stage imaging, CO₂-free pH maintenance, and microfluidic chip loading. FluxMPS™ is engineered against these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> particulate compliance help keep microfluidic channels and open-stage imaging fields free of subvisible debris.

science

CO₂-free by design formulation

Leibovitz's L-15 uses D-galactose (1.8 g/L, not glucose) as the primary carbon source and high amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation; 50 mM HEPES adds a second layer of pH stability.

water_drop

Ultrapure-grade water

18.2 MΩ·cm Type 1 water with low trace-metal and organic-carbon (TOC) content, supporting consistent amino acid and buffer performance from lot to lot.

shield

Low endotoxin release specification

< 0.05 EU/mL (LAL assay, USP <85>) — helps reduce the risk of TLR4-mediated inflammatory activation in endotoxin-sensitive primary cell models.

biotech

HEPES pH stability

HEPES (50 mM, pKa 7.3 at 37°C) resists pH drift during open-air handling, flow cytometry preparation, and atmospheric incubation — without reliance on CO₂/bicarbonate buffering.

tune

Customization on demand

pH, nutrient concentrations, HEPES level, and component modifications available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four validated filtration passes reach a final 0.04 µm pore size, run as two dedicated prefilter + final-filter pairs.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates — including mycoplasma-sized organisms (0.2–0.3 µm) — that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across 4 validated passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved via 0.1 µm mycoplasma-retentive filtration (mycoplasma diameter 0.2–0.3 µm); not tested per lot.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-LL15H-R2X Leibovitz's L-15 2X liquid medium with 50mM HEPES, Quadruple-stage filtration system 0.1 micron x2 and 0.04 micron x2, for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15H-R2X
Applications

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15H-R2X — Leibovitz's L-15 2X + 50mM HEPES — delivers 0.04 µm filtered purity for primary cells and related organ-on-a-chip applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available on request for automated bioreactor and robotic liquid-handling platforms.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Live-Cell Imaging

Open-Stage & CO₂-Free Microscopy

L-15 with galactose maintains pH without CO₂ — the standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning-disk imaging on open-stage microscopes.

ConfocalTIRFLight-sheetOpen-stage
Cell Biology

Primary Cell Dissociation & Transport

CO₂-free L-15 is a standard dissociation and transport buffer for primary cells, tissue pieces, and organoids outside the incubator.

Primary cellsOrganoidsTissue dissociation
Microfluidics

OoC Loading & Priming

CO₂-independent L-15 enables chip loading, priming, and cell seeding outside incubators without pH drift during critical chip assembly steps.

OoC loadingChip primingCell seeding
In Vivo Studies

Intravital Microscopy & Superfusion

L-15 is used as a superfusion medium for intravital microscopy, maintaining pH in open tissue preparations without CO₂ during surgical and imaging procedures.

IntravitalSuperfusionIn vivo
Metabolomics

CO₂-Free Metabolic Studies

The galactose-based energy source enables metabolic studies without CO₂ interference and is commonly used to bias metabolically flexible cells toward oxidative phosphorylation.

OXPHOSGalactose forcingMetabolomics
Neuroscience

Acute Brain Slice & Neuron Imaging

CO₂-free L-15 supports neuronal viability in acute brain slice preparations and dissociated neuron imaging without requiring carbogen (95% O₂/5% CO₂) gassing.

Brain slicesPrimary neuronsNeuronal imaging
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 1.8 g/L D-Galactose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 50mM HEPES | [-] Phenol Red
Appearance Pale yellow colored, clear solution
Carbon source D-Galactose: 1800 mg/L (1.8 g/L)
HEPES 50 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 620–680 mOsm/kg H₂O
Total ingredients 35 components
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent by design — D-galactose and high amino acid buffering maintain pH without gas supplementation.
Raw Materials & Regulatory
Parameter Specification
Grade Microfluidics Suitable (0.04 µm final cut-off)
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Leibovitz's L-15 2X + 50mM HEPES: 35 ingredients verified per lot with CAS numbers for raw-material traceability. D-galactose (not glucose) is the primary carbon source, and high amino acid concentrations plus 50 mM HEPES provide CO₂-free pH buffering. This is a 2X concentrated formulation.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 370.000
Magnesium chloride hexahydrate 7791-18-6 400.000
Magnesium sulfate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Potassium phosphate monobasic 7778-77-0 120.000
Sodium chloride 7647-14-5 16000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 380.240
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 450.000
Glycine 56-40-6 400.000
L-Arginine (free base) 74-79-3 1000.000
L-Asparagine 70-47-3 500.000
L-Cysteine (free base) 52-90-4 240.000
L-Histidine (free base) 71-00-1 500.000
L-Isoleucine 73-32-5 500.000
L-Leucine 61-90-5 250.000
L-Lysine hydrochloride 657-27-2 188.000
L-Methionine 63-68-3 150.000
L-Phenylalanine 63-91-2 250.000
L-Serine 56-45-1 400.000
L-Threonine 72-19-5 600.000
L-Tryptophan 73-22-3 40.000
L-Tyrosine disodium salt 69847-45-6 552.320
L-Valine 72-18-4 200.000
L-Glutamine 56-85-9 600.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.200
Thiamine hydrochloride 67-03-8 2.000
i-Inositol 87-89-8 4.000
OTHERS
D-Galactose 59-23-4 1800.000
HEPES buffer 7365-45-9 11915.000
Sodium pyruvate 113-24-6 1100.000
Custom formulation: Contact support@diagnocine.com for DCP-LL15H-R2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QA at Diagnocine's R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — low trace-metal and organic-carbon content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 620–680 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-LL15H-R2X compares

FluxMPS™ DCP-LL15H-R2X vs. conventional 0.22 µm–filtered Leibovitz's and standard formulations.

Parameter DCP-LL15H-R2X (FluxMPS™) Conventional Leibovitz's L-15
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm) Standard grade (0.22 µm) Standard grade (0.22 µm)
L-15 2X + 50mM HEPES without phenol red — enhanced pH stability for prolonged autofluorescence-free live imaging check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
CO₂-free design check_circle Yes (galactose) check_circle Yes cancel No
HEPES (50 mM) check_circle Yes cancel Usually no cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available on request cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15H-R2X — Leibovitz's L-15 2X + 50mM HEPES.

Yes. DCP-LL15H-R2X is Microfluidics Suitable, processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size. Its CO₂-free design (D-galactose plus high amino acid buffering) makes it well suited to chip loading, priming, and open-stage imaging on OoC and MPS platforms where CO₂ control is impractical.
 
Phenol red is excluded for autofluorescence-free live-cell imaging — the primary application of this L-15 formulation. 50 mM HEPES provides enhanced pH stability during long imaging sessions, making this the preferred base for prolonged open-stage confocal, TIRF, and light-sheet microscopy of primary cells. If a pH indicator is needed for visual monitoring, contact support@diagnocine.com for a phenol-red-containing custom batch.
No. This formulation is CO₂-independent by design — D-galactose and high amino acid concentrations maintain pH without gas supplementation, and 50 mM HEPES adds further buffering capacity.
Yes. FBS (5–10%), serum-free supplements, growth factors, or antibiotics may be added as required. Pre-filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — never 0.04 µm, which retains serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
DCP-LL15H-R2X is released against a specification of < 0.05 EU/mL by LAL assay (USP <85>). This is a batch-level release specification — every manufacturing batch is tested before release and must meet it, along with pH, osmolality, and sterility, prior to shipment.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X + 50mM HEPES in CO₂-free live-cell imaging, primary cell culture, and organ-on-a-chip applications.

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120309
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems and their use in drug screening applications. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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