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FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-LL15H-QPR1X
$55.00
DCP-LL15H-QPR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

Contains 0.9 g/L D-Galactose Contains 25 mM HEPES Contains Calcium Contains Magnesium Without L-Glutamine Without Sodium Pyruvate Without Phenol Red

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium built on 0.9 g/L D-galactose as its carbon source and buffered with 25 mM HEPES for CO2-independent culture — engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under an ISO 13485:2016 quality management system, with final QC and packaging at Diagnocine, Totowa, NJ.

  • Chemically defined Leibovitz's L-15 formulation using 0.9 g/L D-galactose as the carbon source, buffered with 25 mM HEPES for CO2-independent culture
  • Formulated without L-Glutamine, Sodium Pyruvate, and Phenol Red for full control over glutamine supplementation, pyruvate levels, and optical/fluorescence assays
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — Microfluidics Suitable purity architecture
  • Endotoxin release specification: less than 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ, USA
  • 4× BME amino acid and vitamin concentrations supporting long-duration microfluidic and organ-on-a-chip (OoC) perfusion
  • CO2-independent buffering (no sodium bicarbonate) suited to open-well, incubator-free, and portable microfluidic platforms
  • Custom pH, HEPES concentration, salts, and nutrient adjustments available on request — support@diagnocine.com
DCP-LL15H-QPR1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid
  • Carbon Source (Galactose)0.9 g/L
  • L-GlutamineNot added
  • Sodium PyruvateNot added
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)300 - 340 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2-8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

filter_alt

Microchannel-safe purity

A 0.04 µm final filter reduces sub-micron particulates well below what a 0.22 µm filter retains, supporting reliable perfusion in narrow microfluidic channels.

target

Total metabolic control

A single, precisely defined carbon source (0.9 g/L D-galactose) supports controlled glycolytic flux studies, galactose-adaptation assays, and mitochondrial OXPHOS-dependent metabolic research.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696) with controlled trace-metal and organic-carbon (TOC) content.

visibility

Low background for imaging

An ultra-low particulate baseline supports confocal live-cell imaging and biosensor readouts on chip. Note: this formulation contains riboflavin, which contributes native fluorescence — the benefit here is particulate baseline, not spectral background.

science

Rich, stable nutrient profile

4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.

tune

Customization on demand

pH, HEPES concentration, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaches a final pore size of 0.04 µm, run as two dedicated prefilter-plus-final-filter pairs in series — capturing sub-micron particulates and mycoplasma-scale material that 0.22 µm filtration does not.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish in an ISO Class 5 (Class 100) laminar-flow environment.

Performance vs. conventional media

By USP <788> Method 1 (light obscuration), FluxMPS™ delivers approximately 5× fewer particles ≥10 µm compared with standard 0.22 µm filtered media — supporting long-duration perfusion without microchannel occlusion.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final filter — sub-mycoplasma polishing
Sterility & Mycoplasma: 14-day USP <71> sterility tested per lot. The 0.1 µm mycoplasma-retentive filtration stages provide risk mitigation against mycoplasma (typical diameter 0.2–0.3 µm); this is not equivalent to a per-lot USP <63> mycoplasma test.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red 1X Liquid (DCP-LL15H-QPR1X) - Quadruple-stage filtration system (0.1 micron x2 + 0.04 micron x2) for organ-on-a-chip and microfluidic cell culture media - Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → ISO Class 5 aseptic fill.
© Diagnocine® — DCP-LL15H-QPR1X
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is validated for applications where microchannel cleanliness, CO2-independent buffering, and metabolic precision are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For fully automated bioreactor and liquid-handling robotics platforms, Diagnocine also offers this formulation as an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant — a distinct product line from the 0.04 µm Microfluidics Suitable medium described on this page.

  • Total Particulate Exclusion for long-term unattended perfusion runs
  • Valve & Sensor Protection in automated dosing and monitoring systems
  • Extended Perfusion Stability for multi-week bioreactor campaigns

Inquiry Required: The 0.01 µm MPS Grade variant is available by request — contact support@diagnocine.com.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low particulate, CO2-independent media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.

OoCToCBoCMPS
Cancer Biology

Galactose Metabolism & Metabolic Research

A galactose-only carbon source enables studies of the glucose-to-galactose metabolic shift, OXPHOS dependence, and mitochondrial function in cultured cell lines.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-free, CO2-independent perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture outside a CO2 incubator.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Bicarbonate-free, phenol-red-free formulation compatible with Agilent Seahorse XF respiratory assays and NMR metabolomics without background interference.

13C tracingSeahorse XFNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Phenol-red-free medium with low particulate background for confocal microscopy, fluorescent biosensors, and automated imaging on chip.

ConfocalBiosensorsTEER
Technical Specifications

Full technical specification

Every lot of FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 0.9 g/L D-Galactose, 25mM HEPES, Calcium, Magnesium / [-] L-Glutamine, Sodium Pyruvate, Phenol Red
Appearance Clear, colorless solution
pH USP <791> 7.4
Osmolality USP <785> 300 - 340 mOsm/kg H2O
Carbon Source D-Galactose, 0.9 g/L
L-Glutamine Not added
Sodium Pyruvate Not added
Phenol Red Not added
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate matter USP <788> Method 1 Light-obscuration tested; quadruple-stage filtration to 0.04 µm final cut-off
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing standard ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2-8°C)
CO2 requirement Not required (CO2-independent; HEPES-buffered, no sodium bicarbonate)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Traceability Full lot traceability; Certificate of Analysis available
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch; Totowa, NJ, USA
Intended use RUO only
Formulation

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 33 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

INORGANIC SALTS

Component CAS Number mg/L
Calcium chloride dihydrate 10035-04-8 185.000
Magnesium chloride hexahydrate 7791-18-6 200.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Potassium phosphate monobasic 7778-77-0 60.000
Sodium chloride 7647-14-5 8000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 190.120
Component CAS Number mg/L
L-Alanine 56-41-7 225.000
Glycine 56-40-6 200.000
L-Arginine (free base) 74-79-3 500.000
L-Asparagine 70-47-3 250.000
L-Cysteine (free base) 52-90-4 120.000
L-Histidine (free base) 71-00-1 250.000
L-Isoleucine 73-32-5 250.000
L-Leucine 61-90-5 125.000
L-Lysine hydrochloride 657-27-2 94.000
L-Methionine 63-68-3 75.000
L-Phenylalanine 63-91-2 125.000
L-Serine 56-45-1 200.000
L-Threonine 72-19-5 300.000
L-Tryptophan 73-22-3 20.000
L-Tyrosine disodium salt   276.160
L-Valine 72-18-4 100.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Galactose 59-23-4 900.000
HEPES 7365-45-9 5958.000
Custom formulations: pH, galactose concentration, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity with controlled trace-metal and organic-carbon (TOC) content, used as the base for every batch.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving all filtration gains in the final container.

assignment

Micro-Batch Precision

Small-batch production with tight osmolality and pH process control — critical for reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

Light-obscuration particle count testing; approximately 5× fewer particles ≥10 µm vs 0.22 µm filtered media.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 300 - 340 mOsm/kg H2O.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-LL15H-QPR1X compares

Comparison of grade, filtration architecture, and published quality specifications against conventional L-15 media.

Parameter DCP-LL15H-QPR1X (FluxMPS™) Published Supplier Specifications
Grade Microfluidics Suitable Not specified
Formulation 0.9 g/L D-Galactose carbon source; 25 mM HEPES; no L-Glutamine, Sodium Pyruvate, or Phenol Red Not specified
Final filtration pore size 0.04 µm Not specified
Number of filtration stages 4 (Quadruple-stage) Not specified
Mycoplasma-retentive filtration check_circle Not specified
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Not specified
Water quality Ultrapure Type 1, 18.2 MΩ·cm Not specified
Manufacturing QMS ISO 13485:2016 Not specified
Microfluidic channel compatibility check_circle Not specified
Custom formulation check_circle On request Not specified

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid (DCP-LL15H-QPR1X).

Yes. DCP-LL15H-QPR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered medium engineered for organ-on-a-chip, microfluidic, and microphysiological system (MPS) applications. The 0.04 µm final filter reduces sub-micron particulates that can obstruct narrow microfluidic channels.
Four sequential passes (0.1 µm ×2 + 0.04 µm ×2), run as two prefilter-plus-final-filter pairs, reduce sub-micron particulates and provide mycoplasma-retentive filtration well beyond what a single 0.22 µm filter achieves, resulting in approximately 5× fewer particles ≥10 µm by USP <788> Method 1 testing.
L-Glutamine is removed because it degrades in liquid storage; add fresh L-glutamine or a stable dipeptide substitute (e.g., GlutaMAX) at the time of use, typically 2-4 mM. Sodium Pyruvate is removed so researchers can titrate pyruvate independently of the galactose carbon source already present (0.9 g/L). Phenol Red is removed to eliminate optical interference in fluorescence, absorbance, and colorimetric assays. Add each component fresh at your required concentration.
No. This formulation contains no sodium bicarbonate and is buffered with 25 mM HEPES, making it CO2-independent. It is suitable for open-bench, ambient-air, or incubator-free microfluidic culture.
Yes. This basal medium is compatible with FBS, human serum, GlutaMAX, and growth factors such as EGF, FGF, and VEGF. Pre-filter serum and protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter before combining; use a 0.1 µm filter only for defined, protein-free additions. Do not use a 0.04 µm filter on serum or protein-containing supplements, as it will strip out essential lipoproteins and clog rapidly.
Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay (USP <85> Bacterial Endotoxins Test, assay sensitivity 0.005 EU/mL) and must meet the release specification of less than 0.05 EU/mL before it ships. The lot-specific result is reported on the Certificate of Analysis, available at support@diagnocine.com.
Yes. A full CoA is available per lot and includes: lot number, expiry, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), and particulate count (USP <788>). Email support@diagnocine.com with your lot number.
Scientific References

Supporting literature

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120329
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Reitzer LJ, Wice BM, Kennell D. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells. J Biol Chem. 1979;254:2669–2676. doi:10.1016/S0021-9258(17)30124-2
  5. Aguilar-Mahecha A, et al. Galactose adaptation as a model of mitochondrial dependence in cancer cells. Front Oncol. 2020;10:596497. doi:10.3389/fonc.2020.596497
  6. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  7. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  8. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  9. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7

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