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FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o L-Glutamine, Sodium Pyruvate: 2X Liquid

Product#: DCP-LL15H-QP2X
$88.00
DCP-LL15H-QP2X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES — 2X Liquid

Contains Phenol Red Contains HEPES (50 mM) Contains Calcium Contains Magnesium Without L-Glutamine Without Sodium Pyruvate

FluxMPS™ DCP-LL15H-QP2X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Leibovitz's L-15 2X + 50mM HEPES formulation engineered for primary cells, in vivo imaging, and CO2-free applications on microfluidic, organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (50 mM, pKa 7.3 at 37°C) provides robust pH buffering independent of CO2. Formulation: [+] 1.8 g/L D-Galactose, [+] 50mM HEPES | [-] L-Glutamine, [-] Sodium Pyruvate.

  • CO2-free by design: D-galactose energy source + high amino acid buffering — no gas supplementation required
  • 50 mM HEPES (pKa 7.3 at 37°C) provides robust pH buffering independent of CO2
  • Quadruple-stage filtration train: Prefiltration I (0.1 µm) → Final filtration I (0.04 µm) → Prefiltration II (0.1 µm) → Final filtration II — Polish (0.04 µm)
  • Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>)
  • 2X concentrated Leibovitz's L-15 formulation — [-] L-Glutamine, [-] Sodium Pyruvate for independent nutrient control at time of use
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Microfluidics Suitable — 0.04 µm final cut-off validated for microchannel and organ-on-a-chip applications
  • Customization available: pH, HEPES concentration, and nutrient composition on request
CAT. NO.
DCP-LL15H-QP2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 2X + 50mM HEPES — 2X Liquid
  • Carbon source (Galactose)1800 mg/L (1.8 g/L, D-Galactose)
  • L-GlutamineNot added — supplement at time of use
  • Sodium PyruvateNot added — supplement at time of use
  • HEPES50 mM, pKa 7.3 at 37°C
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)620–680 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that interfere with CO2-free pH maintenance and open-stage imaging clarity. FluxMPS™ is built to reduce these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particulate-controlled L-15 for clear open-stage imaging and CO2-free chip loading.

science

CO2-free by design formulation

Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source, together with high amino acid concentrations, for CO2-free pH buffering. This is a 2X concentrated formulation; 50 mM HEPES provides enhanced pH stability for prolonged open-air handling.

water_drop

Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm — low trace-metal and organic-carbon content supports consistent amino acid buffering performance.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL release specification — relevant to primary cells, in which TLR4 activation by LPS/endotoxin can alter phenotype and differentiation outcomes.

device_thermostat

HEPES pH stability

HEPES (50 mM) resists pH rise during open-air handling, flow cytometry preparation, and atmospheric incubation outside a CO2 incubator.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates, including the mycoplasma size range (0.2–0.3 µm), that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma controlled via 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-LL15H-QP2X Leibovitz's L-15 2X + 50mM HEPES quadruple-stage filtration system (0.1 micron x2 + 0.04 micron x2) for organ-on-a-chip and microfluidic cell culture applications - Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15H-QP2X
Applications

CO2-free live imaging and primary cell applications

FluxMPS™ DCP-LL15H-QP2X — Leibovitz's L-15 2X + 50mM HEPES — delivers 0.04 µm filtered purity for primary cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant — adding 0.02 µm and 0.01 µm stages after the 0.04 µm polish — is available on request for automated bioreactor and robotic handling systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Live-Cell Imaging

Open-Stage & CO2-Free Microscopy

L-15 with galactose maintains pH without CO2 — a standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning-disk imaging on open-stage microscopes.

ConfocalTIRFLight-sheetOpen-stage
Cell Biology

Primary Cell Dissociation & Transport

CO2-free L-15 is a standard dissociation and transport buffer for primary cells, tissue pieces, and organoids outside the incubator.

Primary cellsOrganoidsTissue dissociation
Microfluidics

OoC Loading & Priming

CO2-independent L-15 enables chip loading, priming, and cell seeding outside incubators without pH drift during chip assembly steps.

OoC loadingChip primingCell seeding
In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is a standard superfusion medium for intravital microscopy, maintaining pH in open tissue preparations without CO2 during surgical and imaging procedures.

IntravitalSuperfusionIn vivo
Metabolomics

CO2-Free Metabolic Studies

Galactose as the sole simple sugar enables metabolic studies without CO2 interference; galactose forces oxidative phosphorylation in metabolically flexible cells.

OXPHOSGalactose forcingMetabolomics
Neuroscience

Acute Brain Slice & Neuron Imaging

CO2-free L-15 supports neuronal viability in acute brain slice preparations and dissociated neuron imaging without carbogen (95% O2/5% CO2) gassing.

Brain slicesPrimary neuronsNeuronal imaging
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. Available pack sizes: 500 mL, 1000 mL. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, [+] HEPES (50 mM), [+] Calcium, [+] Magnesium | [-] L-Glutamine, [-] Sodium Pyruvate
Appearance Orange-red colored, clear solution
Carbon source D-Galactose — 1800 mg/L (1.8 g/L)
HEPES 50 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 620–680 mOsm/kg H2O
Total ingredients 34
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent by design — D-galactose, high amino acid concentrations, and 50 mM HEPES maintain pH without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Leibovitz's L-15 2X + 50mM HEPES: 34 ingredients verified per lot with CAS numbers for full raw-material traceability. Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO2-free pH buffering. This is a 2X concentrated formulation; 50 mM HEPES provides enhanced pH buffering for prolonged open-stage procedures.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 370.000
Magnesium chloride hexahydrate 7791-18-6 400.000
Magnesium sulfate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Potassium phosphate monobasic 7778-77-0 120.000
Sodium chloride 7647-14-5 16000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 380.240
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 450.000
Glycine 56-40-6 400.000
L-Arginine (free base) 74-79-3 1000.000
L-Asparagine 70-47-3 500.000
L-Cysteine (free base) 52-90-4 240.000
L-Histidine (free base) 71-00-1 500.000
L-Isoleucine 73-32-5 500.000
L-Leucine 61-90-5 250.000
L-Lysine hydrochloride 657-27-2 188.000
L-Methionine 63-68-3 150.000
L-Phenylalanine 63-91-2 250.000
L-Serine 56-45-1 400.000
L-Threonine 72-19-5 600.000
L-Tryptophan 73-22-3 40.000
L-Tyrosine disodium salt 69847-45-6 552.320
L-Valine 72-18-4 200.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
i-Inositol 87-89-8 4.000
D-Galactose 59-23-4 1800.000
HEPES buffer 7365-45-9 11915.000
Phenol red sodium salt 34487-61-1 22.000
Custom formulation: Contact support@diagnocine.com for DCP-LL15H-QP2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — low trace-metal and organic-carbon (TOC) content for consistent formulation performance.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL. See batch-level quality control note below.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 620–680 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-LL15H-QP2X lot at support@diagnocine.com.
Product Comparison

How DCP-LL15H-QP2X compares

FluxMPS™ DCP-LL15H-QP2X vs. conventional 0.22 µm–filtered Leibovitz's formulations.

Parameter DCP-LL15H-QP2X (FluxMPS™) Conventional Leibovitz's L-15
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
Formulation [+] Phenol Red, [+] HEPES (50 mM), [+] Calcium, [+] Magnesium | [-] L-Glutamine, [-] Sodium Pyruvate Base salts, phenol red typically included, no HEPES Base salts, glucose-based, no HEPES
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
CO2-independent design check_circle Yes (galactose) check_circle Yes cancel No
HEPES (50 mM) check_circle Yes cancel Usually no cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15H-QP2X — Leibovitz's L-15 2X + 50mM HEPES.

DCP-LL15H-QP2X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size. Leibovitz's L-15 CO2-free design makes it suited to chip loading, priming, open-stage imaging, and atmospheric OoC platforms where CO2 control is impractical.
 
Both substrates are removed so they can be added fresh at time of use, avoiding glutamine degradation and letting you set your own energy-substrate and nitrogen conditions. 50 mM HEPES provides enhanced pH stability throughout the supplementation procedure.
No. This formulation is CO2-independent by design — D-galactose and high amino acid concentrations, together with 50 mM HEPES, maintain pH without gas supplementation.
Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before adding to the base medium; defined, protein-free additions may use 0.1 µm. Contact support@diagnocine.com for custom co-formulation.
FluxMPS™ DCP-LL15H-QP2X is produced to meet a release specification of < 0.05 EU/mL by LAL assay (USP <85>). Endotoxin is controlled per manufacturing batch; every batch is tested before release. For primary cells, endotoxin can activate TLR4, altering cell phenotype and differentiation outcomes.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X + 50mM HEPES in CO2-free live-cell imaging, primary cell culture, and OoC applications.

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120309
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Rourke AW, et al. Leibovitz's L-15 medium and its applications in CO2-independent live-cell microscopy. Methods Cell Biol. 2007;81:373–390. doi:10.1016/S0091-679X(06)81018-4
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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