FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Formulated with D-galactose in place of glucose and 25 mM HEPES for CO₂-independent culture, without added L-glutamine or sodium pyruvate. Manufactured under ISO 13485:2016 using Ultrapure Type 1 water (18.2 MΩ·cm).
- Quadruple-stage filtration to a 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2) for microchannel-safe purity
- 0.9 g/L D-galactose substituted for glucose — the classical Leibovitz's L-15 design for slower glycolytic acid production under CO₂-independent conditions
- 25 mM HEPES buffering with no added sodium bicarbonate — CO₂ incubator not required
- L-glutamine and sodium pyruvate withheld so you can define your own metabolic supplementation strategy
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under an ISO 13485:2016 quality management system
- Micro-batch production with final QC at Diagnocine, Totowa, NJ
- Custom pH, HEPES, salts & nutrient adjustments available — support@diagnocine.com
- GalactosePresent (0.9 g/L)
- L-GlutamineRemoved ([-])
- Sodium PyruvateRemoved ([-])
- pH (USP <791>)7.4
- Osmolality (USP <785>)300 - 340 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, away from bright light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.
Microchannel-safe purity
A validated 0.04 µm final filter stage helps ensure unobstructed flow through narrow microfluidic channels, reducing the sub-micron particulate load that 0.22 µm filtration leaves behind.
Total metabolic control
D-galactose replaces glucose to slow glycolytic acid production, while L-glutamine and sodium pyruvate are withheld so metabolic tracing and glycolysis studies proceed without background noise from these standard carbon and nitrogen sources.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) with tight trace-metal and organic-carbon control, supporting reproducible baselines for sensitive downstream assays.
Low background for imaging
A low particulate baseline supports confocal live-cell imaging and biosensor readouts. This formulation contains phenol red and riboflavin, so optical background contribution from these components should be assessed for fluorescence- or absorbance-based assays.
Rich, stable nutrient profile
The complete amino acid and vitamin profile of the classical Leibovitz's L-15 formulation is produced under micro-batch precision for tight lot-to-lot consistency across long-duration MPS perfusion.
Customization on demand
pH, glucose/galactose ratio, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
This 1X liquid medium undergoes a validated four-stage sequential filtration process reaching a final pore size of 0.04 µm — a paired prefilter and final-filter architecture, run twice in series, that goes well beyond conventional 0.22 µm filtration.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter cartridge and preserves microchannel chip geometries.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains fine particulates and sub-micron material well below the mycoplasma size range (0.2–0.3 µm) that standard 0.22 µm media does not address.
-
3
0.1 µm Prefiltration II
A second, dedicated prefilter protecting the second 0.04 µm final filter cartridge for redundant bioburden clearance.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter ahead of aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ delivers approximately 5× fewer particles ≥10 µm compared to standard 0.22 µm filtered media — a meaningful difference for long-duration perfusion in narrow microfluidic geometries.
© Diagnocine® — DCP-LL15H-QP1X
Optimized for next-generation cell biology platforms
FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid is validated for applications where microchannel cleanliness, CO₂-independent culture, and metabolic precision are critical.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactor and liquid-handling robotics platforms where valve and sensor protection is paramount.
- Total particulate exclusion beyond the 0.04 µm Microfluidics Suitable tier
- Valve & sensor protection for automated perfusion systems
- Extended perfusion stability for long-duration, unattended runs
Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request this grade for your platform.
Micro Physiological System (MPS) & Chip
Ultra-low particulate, filtration-controlled media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Galactose-based, glucose-free formulation supports Warburg-effect studies, forced oxidative metabolism assays, and aerobic glycolysis research.
iPSC-Derived Models
Ultra-clean, filtration-controlled baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-controlled, CO₂-independent perfusion medium for TEER measurement, endothelial monolayer integrity, and primary cell culture outside a CO₂ incubator.
Metabolic Flux Analysis
Chemically defined, glutamine- and pyruvate-free base for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.
Microscopy & Optical Sensing
Low particulate baseline for confocal microscopy and biosensor readouts on chip; account for phenol red absorbance in fluorescence assay design.
Full technical specification
Every lot of FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid is released against multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] 0.9 g/L D-Galactose, 25mM HEPES, Phenol Red / [-] L-Glutamine, Sodium Pyruvate |
| Appearance | Clear, red-pink solution (phenol red indicator) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 300 - 340 mOsm/kg |
| Glucose | Not present (0.9 g/L D-galactose substituted) |
| L-Glutamine | Not added ([-]) |
| Sodium Pyruvate | Not added ([-]) |
| Phenol Red | 11.000 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL (batch release; see § Manufacturing & Compliance) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, away from bright light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO₂ requirement | Not required (CO₂-independent; HEPES-buffered, bicarbonate-free) |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory | 21 CFR Part 820 (QMSR) aligned |
| Production | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 34 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 185.000 |
| Magnesium chloride hexahydrate | 7791-18-6 | 200.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Potassium phosphate monobasic | 7778-77-0 | 60.000 |
| Sodium chloride | 7647-14-5 | 8000.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 190.120 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Alanine | 56-41-7 | 225.000 |
| Glycine | 56-40-6 | 200.000 |
| L-Arginine (free base) | 74-79-3 | 500.000 |
| L-Asparagine | 70-47-3 | 250.000 |
| L-Cysteine (free base) | 52-90-4 | 120.000 |
| L-Histidine (free base) | 71-00-1 | 250.000 |
| L-Isoleucine | 73-32-5 | 250.000 |
| L-Leucine | 61-90-5 | 125.000 |
| L-Lysine hydrochloride | 657-27-2 | 94.000 |
| L-Methionine | 63-68-3 | 75.000 |
| L-Phenylalanine | 63-91-2 | 125.000 |
| L-Serine | 56-45-1 | 200.000 |
| L-Threonine | 72-19-5 | 300.000 |
| L-Tryptophan | 73-22-3 | 20.000 |
| L-Tyrosine disodium salt | 276.160 | |
| L-Valine | 72-18-4 | 100.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin-5-phosphate sodium salt | 130-40-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| i-Inositol | 87-89-8 | 2.000 |
| OTHERS | ||
| D-Galactose | 59-23-4 | 900.000 |
| HEPES buffer | 7365-45-9 | 5958.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
Manufacturing & compliance
Every batch is subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm with tight trace-metal and organic-carbon control, prepared to minimize contribution to assay background.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains in the final container.
Micro-Batch Precision
Small-batch production targeting tight osmolality (±5 mOsm/kg) and pH (±0.05) process control — supporting reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL.
Filtration Validation
Quadruple-stage 0.1 µm / 0.04 µm filtration train (0.1 µm ×2 + 0.04 µm ×2) validated per manufacturing batch.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 300 - 340 mOsm/kg.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-LL15H-QP1X compares
Differences in grade, filtration, mycoplasma barrier, and endotoxin release specification versus conventional L-15 media.
| Parameter | DCP-LL15H-QP1X (FluxMPS™) | Standard L-15 (0.22 µm) | Competitor L-15 (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Formulation definition | [+] 0.9 g/L Galactose, 25mM HEPES / [-] L-Glutamine, Sodium Pyruvate | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 compliance | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES w/o L-Glutamine, Sodium Pyruvate: 1X Liquid (DCP-LL15H-QP1X).
Supporting literature
- Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120327
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7