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FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o L-Glutamine, Phenol Red: 2X Liquid

Product#: DCP-LL15H-QR2X
$99.00
DCP-LL15H-QR2X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
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FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o L-Glutamine, Phenol Red: 2X Liquid

Contains HEPES Contains Calcium Contains Magnesium Contains Sodium Pyruvate Without L-Glutamine Without Phenol Red

FluxMPS™ DCP-LL15H-QR2X is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Leibovitz's L-15 2X + 50mM HEPES formulation engineered for primary cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (50 mM, pKa 7.3 at 37°C) provides robust pH buffering. Formulation: [+] 1.8 g/L D-Galactose, [+] Sodium Pyruvate, [+] 50mM HEPES | [-] L-Glutamine, [-] Phenol Red.

  • CO2-free by design: D-galactose (1.8 g/L) energy source plus high amino acid buffering eliminate the need for gas supplementation during open-stage procedures
  • 50 mM HEPES (pKa 7.3 at 37°C) delivers robust pH stability independent of CO2 tension
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final polish for microchannel-safe, sub-mycoplasma-range purity
  • Endotoxin release specification: < 0.05 EU/mL by LAL assay (USP <85>)
  • 2X concentrated formulation, free of L-glutamine and phenol red for autofluorescence-reduced live imaging and fresh nitrogen supplementation
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Ultrapure Type 1 water (18.2 MΩ·cm) used throughout manufacture
CAT. NO.
DCP-LL15H-QR2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 — 2X Liquid, 50mM HEPES
  • Media familyLeibovitz's L-15 2X + 50mM HEPES
  • Carbon sourceD-Galactose, 1800 mg/L (1.8 g/L)
  • HEPES50 mM, pKa 7.3 at 37°C
  • Formulation[+] 1.8 g/L D-Galactose, [+] Sodium Pyruvate, [+] 50mM HEPES | [-] L-Glutamine, [-] Phenol Red
  • AppearancePale yellow colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)620 - 680 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • FiltrationQuadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's passes mycoplasma, subvisible particulates, and endotoxin fragments that interfere with CO2-free pH maintenance and live imaging clarity. FluxMPS™ is built to address these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Low-particulate L-15 for clear live imaging and CO2-free chip loading.

science

CO2-free by design formulation

Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO2-free pH buffering. This is a 2X concentrated formulation; 50 mM HEPES provides additional pH stability.

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Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon content, used throughout manufacture.

shield

Low endotoxin release specification

< 0.05 EU/mL — reduces the risk of TLR4-mediated inflammatory signaling that can alter primary cell phenotype in culture.

tune

HEPES pH stability

HEPES (50 mM) resists pH rise during open-air handling, flow cytometry preparation, and atmospheric incubation.

biotech

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish under controlled aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration built into the train; individual lots are not separately tested for mycoplasma by USP <63>.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-LL15H-QR2X Leibovitz's L-15 2X HEPES medium Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip and microfluidic applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15H-QR2X
Applications

CO2-free live imaging and primary cell applications

FluxMPS™ DCP-LL15H-QR2X — Leibovitz's L-15 2X + 50mM HEPES — delivers 0.04 µm filtered, Microfluidics Suitable media for primary cells and related open-atmosphere applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional MPS Grade 0.01 µm (10 nm) ultra nano-filtered variant of this formulation is available on request for automated perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the MPS Grade 0.01 µm variant.

Live-Cell Imaging

Open-Stage & CO2-Free Microscopy

L-15 with galactose maintains pH without CO2 — a standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning-disk imaging on open-stage microscopes.[2]

ConfocalTIRFLight-sheetOpen-stage
Cell Biology

Primary Cell Dissociation & Transport

CO2-free L-15 is a standard dissociation and transport medium for primary cells, tissue pieces, and organoids handled outside the incubator.

Primary cellsOrganoidsTissue dissociation
Microfluidics

OoC Loading & Priming

CO2-independent L-15 supports chip loading, priming, and cell seeding outside incubators without pH drift during chip assembly steps.[3],[4]

OoC loadingChip primingCell seeding
In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is used as a superfusion medium for intravital microscopy, supporting pH maintenance in open tissue preparations without CO2 during surgical and imaging procedures.

IntravitalSuperfusionIn vivo
Metabolomics

CO2-Free Metabolic Studies

The galactose-based energy source supports metabolic studies without CO2 interference; galactose forcing shifts metabolically flexible cells toward oxidative phosphorylation.[9]

OXPHOSGalactose forcingMetabolomics
Neuroscience

Acute Brain Slice & Neuron Imaging

CO2-free L-15 supports neuronal viability in acute brain slice preparations and dissociated neuron imaging without carbogen (95% O2/5% CO2) gassing.

Brain slicesPrimary neuronsNeuronal imaging
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. Pack sizes: 500 mL, 1000 mL. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 1.8 g/L D-Galactose, [+] Sodium Pyruvate, [+] 50mM HEPES | [-] L-Glutamine, [-] Phenol Red
Appearance Pale yellow colored, clear solution
Carbon source D-Galactose: 1800 mg/L (1.8 g/L)
HEPES 50 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 620 - 680 mOsm/kg H2O
Total ingredients 34
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent by design — galactose and high amino acid buffering maintain pH without gas supplementation
Raw Materials & Regulatory Traceability
Parameter Specification
Grade Microfluidics Suitable (0.04 µm final cut-off)
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Leibovitz's L-15 2X + 50mM HEPES: 34 ingredients verified per lot with CAS numbers for full raw-material traceability. This 2X concentrated formulation uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO2-free pH buffering; 50 mM HEPES provides additional pH stability for prolonged open-stage procedures.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 370.000
Magnesium chloride hexahydrate 7791-18-6 400.000
Magnesium sulfate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Potassium phosphate monobasic 7778-77-0 120.000
Sodium chloride 7647-14-5 16000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 380.240
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 450.000
Glycine 56-40-6 400.000
L-Arginine (free base) 74-79-3 1000.000
L-Asparagine 70-47-3 500.000
L-Cysteine (free base) 52-90-4 240.000
L-Histidine (free base) 71-00-1 500.000
L-Isoleucine 73-32-5 500.000
L-Leucine 61-90-5 250.000
L-Lysine hydrochloride 657-27-2 188.000
L-Methionine 63-68-3 150.000
L-Phenylalanine 63-91-2 250.000
L-Serine 56-45-1 400.000
L-Threonine 72-19-5 600.000
L-Tryptophan 73-22-3 40.000
L-Tyrosine disodium salt 69847-45-6 552.320
L-Valine 72-18-4 200.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
i-Inositol 87-89-8 4.000
D-Galactose 59-23-4 1800.000
HEPES buffer 7365-45-9 11915.000
Sodium pyruvate 113-24-6 1100.000
Custom formulation: Contact support@diagnocine.com for DCP-LL15H-QR2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, with controlled trace-metal and organic carbon content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available per lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm) by light obscuration.

Osmolality — USP <785>

Target: 620 - 680 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-LL15H-QR2X compares

FluxMPS™ DCP-LL15H-QR2X vs. conventional 0.22 µm–filtered Leibovitz's and standard DMEM/RPMI formulations, and published supplier endotoxin specifications.

Parameter DCP-LL15H-QR2X (FluxMPS™) Conventional Leibovitz's L-15 (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
L-15 2X + 50mM HEPES — no Glutamine, no Phenol Red check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
CO2-free design check_circle Yes (galactose) check_circle Yes cancel No
HEPES (50 mM) check_circle Yes cancel Usually no cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes — Microfluidics Suitable cancel Higher clogging risk cancel Higher clogging risk
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15H-QR2X — Leibovitz's L-15 2X + 50mM HEPES.

DCP-LL15H-QR2X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size and is Microfluidics Suitable. Leibovitz's L-15 CO2-free design makes it well suited for chip loading, priming, open-stage imaging, and atmospheric OoC platforms where CO2 control is impractical.
FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final pore size and delivering approximately 5× lower particulate counts than single-pass 0.22 µm filtration, with mycoplasma-retentive filtration built into the train.
Phenol red is removed to reduce background for live imaging; L-glutamine is removed so it can be added fresh at time of use, since it degrades in solution over time. 50 mM HEPES provides additional pH stability for extended imaging sessions. This combination is preferred for live-cell imaging immediately after primary cell dissociation with defined nitrogen supplementation.
No. Leibovitz's L-15 is CO2-independent by design: D-galactose and high amino acid buffering maintain pH without gas supplementation, and 50 mM HEPES adds further pH stability during extended open-atmosphere handling.
Yes. FBS (5-10%), serum-free supplements, growth factors, and antibiotics can be added as required. Serum and protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm membrane for supplement filtration, as it will retain serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
DCP-LL15H-QR2X is produced to a release specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch, with every batch tested before release. For primary cells, endotoxin can activate TLR4 signaling and alter phenotype and differentiation outcomes, which is why a low release specification is maintained.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X + 50mM HEPES in CO2-free live-cell imaging, primary cell culture, and OoC applications.

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173-180. doi:10.1093/oxfordjournals.aje.a120309
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  7. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j
  8. Rottem S, Barile MF. Beware of mycoplasmas. Trends Biotechnol. 1993;11:143-151. doi:10.1016/0167-7799(93)90113-P
  9. Park BS, Lee JO. Recognition of lipopolysaccharide pattern by TLR4 complexes. Exp Mol Med. 2013;45:e66. doi:10.1038/emm.2013.97
  10. Aguer C, et al. Galactose enhances oxidative metabolism and reveals mitochondrial dysfunction in human primary muscle cells. PLoS ONE. 2011;6:e28536. doi:10.1371/journal.pone.0028536

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