FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o L-Glutamine: 2X Liquid

FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o L-Glutamine: 2X Liquid

FluxMPS™ DCP-LL15H-Q2X is a Microfluidics Suitable, quadruple-stage ultra-filtered Leibovitz's L-15 2X liquid medium with 50 mM HEPES, formulated without L-glutamine for fresh addition at time of use. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. D-Galactose (not glucose) and elevated amino acid buffering give this formulation CO₂-independent pH stability, ideal for open-stage imaging and organ-on-a-chip (OoC) loading outside the incubator.

• CO₂-free by design: D-galactose energy source (1.8 g/L) plus elevated amino acid buffering — no gas supplementation required
• 50 mM HEPES (pKa 7.3 at 37°C) provides robust pH buffering independent of CO₂
• Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off — Microfluidics Suitable purity architecture
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
• 2X concentrated formulation carrying 1.8 g/L D-galactose and 1100 mg/L sodium pyruvate; L-glutamine omitted for fresh addition to match your protocol
• Retains phenol red (22 mg/L) for visual pH monitoring during open-air handling
• Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's L-15 passes mycoplasma-sized organisms and subvisible particulates that interfere with open-stage imaging and CO₂-free chip loading. FluxMPS™ is built to address these failure modes.

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish.

1. 1

   0.1 µm Prefiltration I

   Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a standard 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge from fouling.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter prior to aseptic fill & finish.

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15H-Q2X — Leibovitz's L-15 2X + 50 mM HEPES — delivers 0.04 µm filtered purity for primary cell and open-stage applications.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion and robotic handling systems — a separate tier from the Microfluidics Suitable product described on this page (see the Grade note above).

• Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
• Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Available pack sizes: 500 mL, 1000 mL.

Full composition (mg/L)

Leibovitz's L-15 2X + 50 mM HEPES: 35 ingredients verified per lot with CAS numbers for full raw-material traceability. D-Galactose replaces glucose as the primary carbon source, and elevated amino acid concentrations provide CO₂-free pH buffering. This is a 2X concentrated formulation; 50 mM HEPES provides enhanced pH buffering for prolonged open-stage procedures.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

How DCP-LL15H-Q2X compares

FluxMPS™ DCP-LL15H-Q2X vs. conventional Leibovitz's L-15 and standard DMEM/RPMI formulations.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15H-Q2X — Leibovitz's L-15 2X + 50 mM HEPES.

Supporting literature

Key publications supporting Leibovitz's L-15 2X + 50 mM HEPES in CO₂-free live-cell imaging, primary cell culture, and OoC applications.

1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120328
2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
7. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
8. Halder G, et al. Live-cell imaging in tissue-on-chip systems. Curr Opin Biomed Eng. 2019;12:34–42. doi:10.1016/j.cobme.2019.09.003