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FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES: 2X Liquid

Product#: DCP-LL15H2X
$88.00
DCP-LL15H2X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES: 2X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES (50 mM) Contains Calcium Contains Magnesium Contains Sodium Pyruvate

FluxMPS™ DCP-LL15H2X is a Microfluidics Suitable, ultra-filtered Leibovitz's L-15 2X + 50mM HEPES formulation engineered for primary cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (50 mM, pKa 7.3 at 37°C) provides robust, CO2-independent pH buffering on top of the D-galactose/high-amino-acid base chemistry of L-15. Formulation: [+] L-Glutamine, [+] Phenol Red, [+] HEPES (50 mM), [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate.

  • CO2-free by design: D-galactose (1.8 g/L) energy source + high amino-acid buffering — no gas supplementation required
  • 50 mM HEPES (pKa 7.3 at 37°C) — double-strength buffering for extended open-stage handling outside the incubator
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
  • Formulation: [+] L-Glutamine, [+] Phenol Red, [+] HEPES (50 mM), [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa NJ
  • Custom pH, HEPES concentration, and nutrient modifications available on request
CAT. NO.
DCP-LL15H2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 — 2X Liquid
  • Carbon source (D-Galactose)1800 mg/L (1.8 g/L)
  • L-Glutamine600 mg/L
  • Sodium Pyruvate1100 mg/L
  • HEPES50 mM, pKa 7.3 at 37°C
  • pH (USP <791>)7.4
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO

Available pack sizes: 500 mL, 1000 mL.

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's passes subvisible particulates and residual mycoplasma-sized organisms that interfere with CO2-free pH maintenance and live imaging clarity. FluxMPS™ is built to reduce these risks.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance — low-particulate L-15 for clear live imaging and CO2-free chip loading.

science

CO2-free by design formulation

Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO2-free pH buffering. This is a 2X concentrated formulation; 50 mM HEPES adds a second, redundant buffering layer.

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Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm — controls trace-metal and organic-carbon (TOC) content, supporting consistent lot-to-lot performance of the L-15 amino-acid buffering system.

shield

Below TLR4 endotoxin threshold

Released to < 0.05 EU/mL — the endotoxin release specification associated with reduced risk of TLR4-mediated inflammatory activation in primary cell models.

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HEPES pH stability

HEPES (50 mM) helps limit pH drift during open-air handling, flow cytometry preparation, and atmospheric incubation.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes — two dedicated 0.1 µm prefilter / 0.04 µm final-filter pairs in series — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates; a dedicated 0.1 µm mycoplasma-retentive stage protecting the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second, independent 0.1 µm prefilter protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate 0.04 µm polishing filter; aseptic fill and finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma control is by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-LL15H2X Leibovitz's L-15 2X plus 50mM HEPES Quadruple-stage filtration system, 0.1 micron times 2 plus 0.04 micron times 2, for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15H2X
Applications

CO2-free live imaging and primary cell applications

FluxMPS™ DCP-LL15H2X — Leibovitz's L-15 2X + 50mM HEPES — delivers 0.04 µm filtered purity for primary cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Live-Cell Imaging

Open-Stage & CO2-Free Microscopy

L-15 + galactose maintains pH without CO2 — a standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning-disk imaging on open-stage microscopes.

ConfocalTIRFLight-sheetOpen-stage
Cell Biology

Primary Cell Dissociation & Transport

CO2-free L-15 is a standard dissociation and transport buffer for primary cells, tissue pieces, and organoids outside the incubator.

Primary cellsOrganoidsTissue dissociation
Microfluidics

OoC Loading & Priming

CO2-independent L-15 enables chip loading, priming, and cell seeding outside incubators without pH drift during critical chip-assembly steps.

OoC loadingChip primingCell seeding
In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is used as a superfusion medium for intravital microscopy, supporting pH stability in open tissue preparations without CO2 during surgical and imaging procedures.

IntravitalSuperfusionIn vivo
Metabolomics

CO2-Free Metabolic Studies

Galactose-based energy source enables metabolic studies without CO2 interference; galactose forces oxidative phosphorylation (OXPHOS) in metabolically flexible cells.

OXPHOSGalactose forcingMetabolomics
Neuroscience

Acute Brain Slice & Neuron Imaging

CO2-free L-15 supports neuronal viability in acute brain-slice preparations and dissociated neuron imaging without requiring carbogen (95% O2/5% CO2) gassing.

Brain slicesPrimary neuronsNeuronal imaging
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] HEPES (50 mM), [+] Calcium, [+] Magnesium, [+] Sodium Pyruvate
Appearance Orange-Red colored, clear solution
Carbon source D-Galactose — 1800 mg/L (1.8 g/L)
HEPES 50 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 36
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent by design — D-galactose + high amino-acid buffering, reinforced by 50 mM HEPES. No gas supplementation required.
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Leibovitz's L-15 2X + 50mM HEPES: 36 ingredients verified per lot with CAS numbers for full raw-material traceability. Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO2-free pH buffering. This is a 2X concentrated formulation. 50 mM HEPES provides enhanced pH buffering for prolonged open-stage procedures.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 370.000
Magnesium chloride hexahydrate 7791-18-6 400.000
Magnesium sulfate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Potassium phosphate monobasic 7778-77-0 120.000
Sodium chloride 7647-14-5 16000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 380.240
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 450.000
Glycine 56-40-6 400.000
L-Arginine (free base) 74-79-3 1000.000
L-Asparagine 70-47-3 500.000
L-Cysteine (free base) 52-90-4 240.000
L-Histidine (free base) 71-00-1 500.000
L-Isoleucine 73-32-5 500.000
L-Leucine 61-90-5 250.000
L-Lysine hydrochloride 657-27-2 188.000
L-Methionine 63-68-3 150.000
L-Phenylalanine 63-91-2 250.000
L-Serine 56-45-1 400.000
L-Threonine 72-19-5 600.000
L-Tryptophan 73-22-3 40.000
L-Tyrosine disodium salt 69847-45-6 552.320
L-Valine 72-18-4 200.000
L-Glutamine 56-85-9 600.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.200
Thiamine hydrochloride 67-03-8 2.000
i-Inositol 87-89-8 4.000
OTHERS
D-Galactose 59-23-4 1800.000
HEPES buffer 7365-45-9 11915.000
Phenol red sodium salt 34487-61-1 22.000
Sodium pyruvate 113-24-6 1100.000
Custom formulation: Contact support@diagnocine.com for DCP-LL15H2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — controls trace-metal and organic-carbon (TOC) content for consistent formulation performance.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every batch.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available for any DCP-LL15H2X lot at support@diagnocine.com.
Product Comparison

How DCP-LL15H2X compares

FluxMPS™ DCP-LL15H2X vs. conventional 0.22 µm–filtered Leibovitz's and standard DMEM/RPMI formulations.

Parameter DCP-LL15H2X (FluxMPS™) Conventional Leibovitz's L-15
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm) Not applicable Not applicable
L-15 2X + 50mM HEPES — double-strength HEPES for prolonged CO2-free live imaging and primary cell transport check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stages) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15H2X — Leibovitz's L-15 2X + 50mM HEPES.

Yes. DCP-LL15H2X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, and its CO2-free Leibovitz's L-15 design makes it well suited to chip loading, priming, and open-stage imaging on OoC and microphysiological system (MPS) platforms where continuous CO2 control is impractical. This product is Microfluidics Suitable at the 0.04 µm cut-off.
FluxMPS™ uses four sequential filtration passes — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish) — reaching a 0.04 µm final cut-off versus the 0.22 µm typical of conventional media. This delivers approximately 5× lower particulate counts by count, with mycoplasma-retentive (0.1 µm) filtration built into the process train.
Leibovitz's L-15 uses D-galactose (1.8 g/L) instead of glucose as its carbon source, combined with high amino-acid concentrations, so pH is maintained without CO2. If your cell model requires glycolytic flux or a glucose-dependent phenotype, glucose can be added on request. L-glutamine (600 mg/L), sodium pyruvate (1100 mg/L) and HEPES (50 mM) are already included in this formulation, so no further addition of these is needed for standard use. Contact support@diagnocine.com for custom formulation.
No. This formulation is CO2-independent by design: D-galactose and high amino-acid buffering, reinforced by 50 mM HEPES (pKa 7.3 at 37°C), maintain pH without a CO2 atmosphere. No gas supplementation is required.
Yes. FBS (5–10%), serum-free supplements, growth factors, or antibiotics can be added as required. Serum and other protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; defined, protein-free additions may use a 0.1 µm membrane. Contact support@diagnocine.com for custom co-formulation.
DCP-LL15H2X is released to meet an endotoxin specification of < 0.05 EU/mL, verified by LAL assay per USP <85> (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). This specification is controlled per manufacturing batch: every batch is tested before release and must meet this limit along with pH, osmolality, sterility, and appearance criteria. A Certificate of Analysis is available on request.
Yes. A full CoA is issued per batch and covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X + 50mM HEPES in CO2-free live-cell imaging, primary cell culture, and OoC applications.

  1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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