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FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES: 1X Liquid

Product#: DCP-LL15H1X
$49.50
DCP-LL15H1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains 25mM HEPES Contains Calcium Contains Magnesium Contains Sodium Pyruvate

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). The quadruple-stage train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Leibovitz's L-15 formulation buffered with 25 mM HEPES and D-galactose (0.9 g/L) in place of glucose, supporting CO2-independent culture in ambient air
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off for microchannel-safe purity
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
  • Bicarbonate-free, HEPES-buffered formulation — no CO2 incubator required
  • Manufactured under an ISO 13485:2016 quality management system with ISO Class 5 aseptic fill & finish
  • 4× BME amino acid & vitamin concentrations for robust long-duration MPS perfusion
  • Custom pH, galactose, HEPES, salts & nutrient levels available on request — support@diagnocine.com
DCP-LL15H1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Leibovitz's L-15 Medium with 25mM HEPES: 1X Liquid
  • Carbon Source (D-Galactose)900 mg/L ([+])
  • L-Glutamine300 mg/L ([+])
  • Sodium Pyruvate550 mg/L ([+])
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)300 - 340 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2
  • Storage2-8°C, away from bright light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2–8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma-scale particles (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and complicating metabolic assays. FluxMPS™ closes that gap.

filter_alt

Microchannel-safe purity

A 0.04 µm final filter and USP <788> particulate testing support unobstructed flow in narrow microfluidic channels.

target

Total metabolic control

Defined galactose- and pyruvate-based carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) to reduce trace-metal ions and total organic carbon that could interfere with sensitive biosensor and TEER measurements.

visibility

Low background for imaging

Low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.

science

Rich, stable nutrient profile

4× BME amino acid and vitamin concentrations with micro-batch precision — consistent lot-to-lot composition for long-duration MPS perfusion.

tune

Customization on demand

pH, galactose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaches a final pore size of 0.04 µm — capturing sub-visible particulates and bioburden that a standard 0.22 µm filter cannot.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm cartridge and preserves microchannel chip geometries downstream.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass. Retains fine particulates and sub-micron bioburden, including mycoplasma-scale particles (0.2–0.3 µm), that a standard 0.22 µm filter cannot capture.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated 0.1 µm prefilter, protecting the second 0.04 µm cartridge and providing redundant bioburden reduction.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter. Aseptic fill and finish in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

0.04
µm final filter cut-off
4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility & Mycoplasma: 14-day USP <71> sterility tested per manufacturing batch. Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot per USP <63>). Typical mycoplasma diameter is 0.2–0.3 µm, above the 0.04 µm final cut-off.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES: 1X Liquid (DCP-LL15H1X) ? Quadruple-stage filtration system (0.1 μm ×2 + 0.04 μm ×2) for organ-on-a-chip and microfluidic MPS cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → ISO Class 5 aseptic fill.
© Diagnocine® — DCP-LL15H1X
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactors and robotic liquid-handling systems where absolute particulate exclusion is critical.

  • Total Particulate Exclusion — 0.01 µm six-stage cascade for zero-tolerance microfluidic networks
  • Valve & Sensor Protection — minimizes fouling of microvalves, flow sensors, and optical windows
  • Extended Perfusion Stability — supports weeks-long unattended perfusion runs

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to discuss requirements.

Microfluidics

Micro Physiological System (MPS) & Chip

Low-particulate, filtration-controlled media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.

OoCToCBoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Galactose-based carbon source supports Warburg-effect studies, aerobic glycolysis, and cancer cell metabolism research requiring controlled substrate availability.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Low-particulate, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Low-particulate perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate baseline supports confocal microscopy, fluorescent biosensors, and automated imaging on chip.

ConfocalBiosensorsTEER
Technical Specifications

Full technical specification

Every lot of FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, Phenol Red, HEPES, Calcium, Magnesium, Sodium Pyruvate
Appearance Clear solution (phenol red indicator present)
pH USP <791> 7.4
Osmolality USP <785> 300 - 340 mOsm/kg H2O
Carbon Source (D-Galactose) 900 mg/L ([+])
L-Glutamine 300 mg/L ([+])
Sodium Pyruvate 550 mg/L ([+])
Phenol Red 11 mg/L ([+])
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, away from bright light
Freeze–thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2–8°C)
CO2 requirement Not required (CO2-independent); HEPES-buffered, bicarbonate-free formulation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Traceability Full lot traceability; CoA available on request
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production Micro-batch; Totowa, NJ, USA
Intended use RUO only
Formulation

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 36 components across 4 formulation categories (Inorganic Salts, Amino Acids, Vitamins, Others). All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 185.000
Magnesium chloride hexahydrate 7791-18-6 200.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Potassium phosphate monobasic 7778-77-0 60.000
Sodium chloride 7647-14-5 8000.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 190.120
Component CAS Number mg/L
AMINO ACIDS
L-Alanine 56-41-7 225.000
Glycine 56-40-6 200.000
L-Arginine (free base) 74-79-3 500.000
L-Asparagine 70-47-3 250.000
L-Cysteine (free base) 52-90-4 120.000
L-Glutamine 56-85-9 300.000
L-Histidine (free base) 71-00-1 250.000
L-Isoleucine 73-32-5 250.000
L-Leucine 61-90-5 125.000
L-Lysine hydrochloride 657-27-2 94.000
L-Methionine 63-68-3 75.000
L-Phenylalanine 63-91-2 125.000
L-Serine 56-45-1 200.000
L-Threonine 72-19-5 300.000
L-Tryptophan 73-22-3 20.000
L-Tyrosine disodium salt   276.160
L-Valine 72-18-4 100.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin-5-phosphate sodium salt 130-40-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
D-Galactose 59-23-4 900.000
HEPES buffer 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 11.000
Sodium pyruvate 113-24-6 550.000
i-Inositol 87-89-8 2.000
Custom formulations: pH, galactose, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every batch subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, low total organic carbon (TOC). Reduces trace-metal and organic contaminants that could interfere with sensitive biosensor and TEER systems.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves all filtration gains in the final container.

assignment

Micro-Batch Precision

Small-batch production for tight lot-to-lot consistency in pH, osmolality, and endotoxin — critical for reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL assay per USP <85> Bacterial Endotoxins Test. Release specification: < 0.05 EU/mL. Tested per manufacturing batch, not per unit.

Sterility — USP <71>

14-day sterility test per USP <71>; no growth required for lot release.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 300 - 340 mOsm/kg H2O.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
CoA Request: Available at no charge for any production lot of FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES: 1X Liquid (DCP-LL15H1X). Email support@diagnocine.com.
Product Comparison

How DCP-LL15H1X compares

Published specification comparisons for filtration, formulation, and QC depth relevant to MPS and microfluidic applications.

Parameter DCP-LL15H1X (FluxMPS™) Other Suppliers (published specifications)
Grade Microfluidics Suitable Not specified
Formulation Galactose-based, HEPES-buffered, bicarbonate-free Not specified
Final filtration pore size 0.04 µm Not specified
Number of filtration stages 4 (quadruple-stage) Not specified
Mycoplasma-retentive filtration check_circle Not specified
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate testing Tested per lot; no numeric result published Not specified
Water quality Ultrapure Type 1, 18.2 MΩ·cm Not specified
Manufacturing QMS ISO 13485:2016 Not specified
Microfluidic channel compatibility check_circle Not specified
Custom formulation check_circle On request Not specified

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Leibovitz's L-15 Medium with 25mM HEPES: 1X Liquid (DCP-LL15H1X).

DCP-LL15H1X is engineered for organ-on-a-chip, microfluidic, and MPS applications. The quadruple-stage filtration train, finishing at 0.04 µm, removes sub-micron particulates and reduces mycoplasma-scale bioburden that can clog microchannels in standard 0.22 µm media.
Four sequential stages (0.1 µm ×2 + 0.04 µm ×2) reach a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional filtration, and fine enough to retain mycoplasma-scale particles (0.2–0.3 µm) that pass through standard filters.
Leibovitz's L-15 is formulated with D-galactose (0.9 g/L) and 25 mM HEPES rather than glucose and sodium bicarbonate, enabling stable pH control in ambient (non-CO2) air — ideal for open-bench microscopy and microfluidic perfusion. If your cell line requires glucose metabolism, contact support@diagnocine.com to discuss a custom glucose-containing formulation.
No. DCP-LL15H1X is bicarbonate-free and buffered with 25 mM HEPES, so it is formulated for CO2-independent culture in ambient air incubators.
Yes. DCP-LL15H1X is a basal medium compatible with FBS (2–10%), human serum, EGF, FGF, VEGF, and antibiotics. Filter serum- or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before combining; do not use a 0.04 µm filter for protein-containing supplements, as it will retain proteins and lipoproteins.
Release specification: < 0.05 EU/mL by LAL assay, USP <85> Bacterial Endotoxins Test, tested per manufacturing batch. Lot-specific results are reported on the Certificate of Analysis — request at support@diagnocine.com.
Yes — a full CoA per lot includes lot number, expiry, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), and mycoplasma filtration status. Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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