LDS Sample Buffer without Reducing Agent [4X]
Cat No.: DCP-LDSSB-RA4X
Size: 25 mL & 100 mL
Non-Sterile; Ultrapure, Filtered 0.45-micron membrane ONCE.
Description
LDS Sample Buffer is designed to provide optimal protein denaturation and separation for various gel electrophoresis applications, particularly with Bis-Tris and Tris-Acetate gel systems. Lithium Dodecyl Sulfate (LDS) denatures proteins and gives them a uniform negative charge. LDS has a slightly alkaline pH which provides optimal conditions for reduction of protein disulfide bonds, denaturation of proteins. The alkaline pH ensures that most proteins are negatively charged, which is crucial for their migration towards the positive electrode during electrophoresis. Alkaline pH helps prevent disulfide bond shuffling, which can occur under certain conditions like heat or agitation. It helps to minimize incorrect disulfide bonds formation. This buffer does not contain a reducing agent which provides flexibility in experimental design, allowing researchers to choose whether to maintain or reduce disulfide bonds based on their specific research needs. The buffer denatures proteins by disrupting non-covalent bonds, but without a reducing agent, it does not break disulfide bonds. This means that proteins may retain some of their tertiary or quaternary structures if disulfide bonds are present. It contains glycerol to increase sample density and tracking dyes (such as Coomassie G250 and phenol red) for visualization.
Applications
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Native Gel Electrophoresis: While LDS is typically used for denaturing gels, using it without a reducing agent can provide insights similar to native gel electrophoresis, focusing on the native disulfide-bonded forms of proteins.
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Non-Reducing Conditions: Useful for applications where the intact disulfide bonds are necessary for the analysis, such as when studying protein complexes or when the reduction of disulfide bonds is not desired.
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Structural Studies: Helps in understanding the structural integrity of proteins where disulfide bonds play a crucial role in maintaining the protein's conformation.
Advantages:
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Compatible with Bis-Tris and Tris-Acetate gels.
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Provides sharp dye front with both MES and MOPS running buffers.
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Ensures small peptides don't run off the gel.
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LDS prevents protein degradation better than SDS during sample preparation.
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Coomassie G250 gives a sharper dye front than bromophenol blue.
Composition:
| Ingredients |
Concentration |
| Tris-HCl |
988 mM |
| EDTA |
2.04 mM |
| LDS |
8% |
| Glycerol |
40% |
| Coomassie blue |
0.88% |
| Phenol red |
0.7 mM |
Quality Control:
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
