LDS Sample Buffer with Reducing Agent [4X]
FluxMPS™ LDS Sample Buffer with Reducing Agent [4X]
A ready-to-use, 4X concentrated LDS (lithium dodecyl sulfate) sample buffer formulated with TCEP as the reducing agent for denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Membrane-filtered once at 0.45 µm and QC-tested for DNase/RNase activity, it delivers a consistent, low-background loading buffer with built-in Coomassie Blue and phenol red tracking dyes for reliable, size-based protein separation.
- Membrane-filtered once through a 0.45 µm filter for particulate-reduced, clean electrophoretic runs
- Contains TCEP as the reducing agent — cleaves disulfide bonds without the odor of DTT/BME
- Balanced Tris-HCl buffering system holds pH in the 8-8.4 range
- Built-in Coomassie Brilliant Blue and phenol red tracking dyes to monitor run progress
- Glycerol density agent ensures reliable sample loading into gel wells
- DNase and RNase activity: none detected after 18 hr incubation at room temperature
- Manufactured under ISO 13485-certified, CE-approved facilities; final QA/packaging at DiagnoCine's Totowa, NJ, USA Quality Testing Center
- Formulation, pH, and additive customization available on request — contact support@diagnocine.com
- pH8-8.4
- Concentration Format4X
- Reducing AgentTCEP, 50 mM
- DetergentLDS, 8%
- Tracking DyesCoomassie Blue + Phenol Red
- Filtration0.45 µm membrane, once
- SterilityNon-sterile
- DNase / RNaseNot detected
- Storage4°C
- Shelf Life6 months
Engineered for clean, reproducible protein electrophoresis
Inconsistent sample buffers introduce particulate streaking, incomplete reduction, and lot-to-lot drift in band resolution. FluxMPS™ LDS Sample Buffer with Reducing Agent [4X] is filtered, QC-tested, and formulated to a defined pH so every gel and every blot behaves the same way, run after run.
Membrane-Filtered Purity
Passed once through a 0.45 µm membrane to reduce particulate carryover that can streak or smear a denaturing gel.
TCEP-Based Complete Reduction
TCEP at 50 mM cleaves intra- and inter-molecular disulfide bonds for thorough, odorless protein reduction ahead of SDS-PAGE.
Balanced Tris-HCl Buffering
Tris-HCl at 988 mM holds the working pH in the 8-8.4 range for consistent denaturation and uniform protein charge.
Built-In Migration Tracking
Coomassie Brilliant Blue and phenol red let you watch the run progress and load wells with confidence via the glycerol density agent.
QC-Tested, Defined Composition
Every lot is tested for DNase and RNase activity, with none detected after 18 hr incubation at room temperature.
Customization on Demand
Alternate concentrations, additives, or pH available on inquiry — contact support@diagnocine.com.
Single-stage 0.45 µm filtration
This sample buffer is passed once through a 0.45 µm membrane to reduce particulate content ahead of electrophoresis. The buffer is supplied non-sterile and is intended for laboratory sample preparation, not for cell culture or aseptic applications.
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0.45 µm Membrane Filtration
A single pass through a 0.45 µm membrane removes coarse particulates prior to fill, supporting clean, streak-free electrophoretic runs.
Why membrane filtration matters here
Particulate carryover in a loading buffer can appear as streaking or background noise on a denaturing gel. A single 0.45 µm membrane pass reduces this risk before the buffer reaches your samples.
© Diagnocine® — DCP-LDSSBRA4X
Where this sample buffer is used
Formulated for preparing protein samples for denaturing electrophoresis and downstream protein analysis workflows.
Automated & Robotic Sample Preparation
The 4X liquid concentrate format and membrane-filtered composition make this buffer suitable for manual and automated/robotic liquid-handling sample-prep workflows feeding SDS-PAGE and capillary-based (microfluidic) Western blot instruments.
- Consistent Viscosity: Glycerol content supports reliable pipetting by automated liquid handlers
- Clean Formulation: 0.45 µm membrane filtration reduces particulate that can interfere with capillary or microfluidic sample channels
- Ready-to-Use Concentrate: 4X format simplifies dilution protocols in automated sample-prep scripts
Inquiry Required: for alternate concentrations, additives, or custom fill volumes for automated platforms, contact support@diagnocine.com.
SDS-PAGE Sample Preparation
Prepares protein samples for denaturing polyacrylamide gel electrophoresis, particularly where reduced thiol groups must be maintained.
Western Blotting
Denatured and reduced proteins prepared with this buffer transfer more readily to membranes and remain accessible for antibody binding.
Composition, Purity & Molecular Weight Analysis
Supports analysis of protein composition, purity, and apparent molecular weight, including identification and quantification within complex mixtures.
Reduced vs. Non-Reduced Comparisons
Enables side-by-side comparison of reduced and non-reduced protein samples, informing the role of disulfide bonds in structure and function.
Capillary / Microfluidic Western Platforms
Compatible with sample-preparation workflows feeding automated capillary-based Western blot systems that use LDS/TCEP-type sample buffers.
Membrane Protein Solubilization
LDS detergent effectively solubilizes proteins, including membrane proteins that are otherwise difficult to bring into solution.
Product specifications
Measured and declared values for FluxMPS™ LDS Sample Buffer with Reducing Agent [4X], DCP-LDSSBRA4X.
| Parameter | Specification |
|---|---|
| Formulation | Tris-HCl, EDTA, LDS, glycerol, Coomassie Brilliant Blue, phenol red, TCEP |
| Appearance | Dark Blue Liquid |
| pH | 8-8.4 |
| Concentration Format | 4X |
| Parameter | Specification |
|---|---|
| Sterility | Non-sterile; filtered 0.45 µm membrane once |
| DNase Activity | None detected (18 hr incubation, room temperature) |
| RNase Activity | None detected (18 hr incubation, room temperature) |
| Manufacturing Facility | ISO 13485 certified, CE-approved |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 6 months |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified facility |
| Regulatory Alignment | CE-approved facility |
| Final QA & Packaging | DiagnoCine R&D and Quality Testing Center; customization/assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Every component and concentration in FluxMPS™ LDS Sample Buffer with Reducing Agent [4X], as declared on the product's release specification.
| Component | CAS Number | Concentration |
|---|---|---|
| Tris-HCl | 1185-53-1 | 988 mM |
| EDTA | 60-00-4 | 2.04 mM |
| LDS (Lithium Dodecyl Sulfate) | 2044-56-6 | 8% |
| Glycerol | 56-81-5 | 40% |
| Coomassie Brilliant Blue | 0.88% | |
| Phenol Red | 143-74-8 | 0.7 mM |
| TCEP | 51805-45-9 | 50 mM |
Manufacturing & compliance
Manufactured under ISO 13485-certified, CE-approved facilities with final packaging, QA, and testing completed at DiagnoCine's own Quality Testing Center.
ISO 13485:2016 QMS
Manufactured under an ISO 13485-certified quality management system.
CE-Approved Facility
Produced by suppliers of DiagnoCine Precision operating under CE-approved manufacturing practices.
Final QA at DiagnoCine R&D Center
Final packaging, quality assurance, and testing are completed at the DiagnoCine R&D and Quality Testing Center.
Custom Assembly in Totowa, NJ
Specific customization requests and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
DNase Activity Testing
None detected after incubation of plasmid DNA with this product for 18 hr at room temperature.
RNase Activity Testing
No RNase activity detected after incubation of ribosomal RNA with this product for 18 hr at room temperature.
pH Verification
Each lot is verified to fall within the 8-8.4 pH specification.
Documentation & CoA
Contact support for lot-specific documentation.
How DCP-LDSSBRA4X compares
A look at how this TCEP-based, membrane-filtered sample buffer compares to conventional LDS/SDS sample buffers.
| Parameter | DCP-LDSSBRA4X (FluxMPS™) | Conventional Sample Buffer | Standard Alternative |
|---|---|---|---|
| TCEP-based reducing agent | check_circle | cancel | cancel |
| Built-in Coomassie Blue + phenol red tracking dyes | check_circle | cancel | check_circle |
| Ready-to-use 4X concentrate | check_circle | check_circle | check_circle |
| 0.45 µm membrane filtration | check_circle | cancel | cancel |
| ISO 13485-certified manufacturing | check_circle | cancel | check_circle |
| CE-approved manufacturing facility | check_circle | cancel | cancel |
| DNase / RNase tested per lot | check_circle | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Answers to common questions about DCP-LDSSBRA4X.
Supporting literature
Curated peer-reviewed literature relevant to SDS-PAGE sample preparation, TCEP-based reduction, and Coomassie-based tracking dyes.
- Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970. doi:10.1038/227680a0
- Shapiro AL, Vinuela E, Maizel JV. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967. doi:10.1016/0006-291X(67)90391-9
- Burnette WN. Western blotting: electrophoretic transfer of proteins from SDS-polyacrylamide gels to unmodified nitrocellulose. Anal Biochem. 1981. doi:10.1016/0003-2697(81)90281-5
- Han JC, Han GY. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem. 1994. doi:10.1006/abio.1994.1112
- Getz EB, Xiao M, Chakrabarty T, Cooke R, Selvin PR. A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry. Anal Biochem. 1999. doi:10.1006/abio.1999.4203
- Neuhoff V, Arold N, Taube D, Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue. Electrophoresis. 1988. doi:10.1002/elps.1150090603
- O'Keefe DO. Effects of sample buffer composition on apparent molecular weights of proteins during SDS-polyacrylamide gel electrophoresis. Anal Biochem. 1994. doi:10.1006/abio.1994.1245
- Kane LA, Van Eyk JE. Post-translational modifications and their relevance to Western blotting sample preparation. Circ Res. 2009. doi:10.1161/CIRCRESAHA.109.204370