UltraClean Krebs-Ringer Bicarbonate Buffer with Calcium chloride, 10mM HEPES, 0.2% Bovine Serum Albumin and Sodium bicarbonate, without Glucose, 1X (Liquid), 0.04um sterile filtered

FluxMPS™ UltraClean Krebs-Ringer Bicarbonate Buffer with Calcium Chloride, 10mM HEPES, 0.2% Bovine Serum Albumin and Sodium Bicarbonate, without Glucose, 1X (Liquid), 0.04 µm Sterile Filtered

An MPS-grade, glucose-free Krebs-Ringer Bicarbonate Buffer formulated at pH 7.4 with 10 mM HEPES, calcium chloride, sodium bicarbonate, and 0.2% bovine serum albumin. Quadruple-stage filtration (0.1 µm membrane twice and 0.04 µm membrane twice) delivers microchannel-safe purity for Glucose-Stimulated Insulin Secretion (GSIS) assays, islet perifusion, primary adipocyte lipolysis studies, and microfluidic perfusion platforms.

• Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice for microchannel-safe purity
• Endotoxin NMT 1 EU/mL, verified per USP specification
• Defined pH 7.4 with 10 mM HEPES buffering (pKa 7.3 at 37°C)
• Osmolality 250.00 - 290.00 mOsm/Kg H₂O
• Formulated without glucose — purpose-built for GSIS and non-glucose functional assays
• Manufactured with ultrapure Type 1 water (18.2 MΩ·cm)
• Compatible with organ-on-a-chip, tissue-on-a-chip, and microfluidic perfusion systems
• Custom pH, molarity, and additive formulations available on request

Engineered where standard KRB buffers fail

Conventional 0.22 µm-filtered Krebs-Ringer Bicarbonate Buffer carries subvisible particulates and variable ionic content that accumulate in microfluidic channels, drift in pH and osmolality, and elevate assay background in insulin secretion and imaging workflows. FluxMPS™ buffer is engineered to remove these failure modes at the source.

Quadruple-stage filtration system

This buffer is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice, a four-pass architecture designed to remove particulates and bioburden well beyond what a single-pass conventional filter achieves.

1. 1

   0.1 µmPre-filtration I

   Removes large particulates and aggregates, extending the working life of the downstream filters.

2. 2

   0.04 µmPre-filtration II

   Retains fine particulates and bioburden ahead of final sterile filtration.

3. 3

   0.1 µmSterile-filtration I

   A second 0.1 µm pass provides redundant sterile filtration.

4. 4

   0.04 µmSterile-filtration II — Final Polish

   A second 0.04 µm pass performed in an ISO Class 5 (Class 100) aseptic fill environment gives the final polish. This pore size is well below the approximately 0.2 micron diameter of the smallest known mycoplasma organisms, supporting mycoplasma contamination control.

Where this buffer performs

Krebs-Ringer Bicarbonate Buffer without glucose supports tissue cell culture washing, primary adipocyte preparation, lipolysis assays, islet pre-perifusion for viability and functionality studies, pituitary bioassays, and Glucose-Stimulated Insulin Secretion (GSIS) assays that require a non-glucose baseline.

Automated Bioreactors & Robotics

For automated perfusion bioreactors and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on inquiry, engineered for total particulate exclusion in sensitive fluidic hardware.

• Total Particulate Exclusion for closed-loop perfusion systems
• Valve & Sensor Protection against sub-micron fouling
• Extended Perfusion Stability across long-duration runs

Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade of this buffer.

Detailed specifications

All values below are declared for this product as supplied by DiagnoCine Precision.

Full composition (mg/L)

Component concentrations are expressed in mg/L as released per manufacturing lot.

Manufacturing & compliance

This buffer is manufactured under ISO 13485-certified and CE-approved facilities, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.

How DCP-KRBBWOG1X compares

A comparison against conventional Krebs-Ringer Bicarbonate Buffer preparations.

Frequently asked questions

Answers to common questions about DCP-KRBBWOG1X.

Supporting literature

Curated references relevant to Krebs-Ringer Bicarbonate Buffer use in cell physiology and microfluidic applications.

1. Ringer S. A further contribution regarding the influence of the different constituents of the blood on the contraction of the heart. J Physiol. 1885. doi:10.1113/jphysiol.1885.sp000184
2. Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
3. Rorsman P, Ashcroft FM. Pancreatic beta-cell electrical activity and insulin secretion: of mice and men. Physiol Rev. 2018. doi:10.1152/physrev.00008.2017
4. Rodbell M. Metabolism of isolated fat cells. J Biol Chem. 1964. doi:10.1016/S0021-9258(18)91674-1
5. Bowe DB, et al. Static incubation for the measurement of glucose-stimulated insulin secretion from mouse islets. J Vis Exp. 2014. doi:10.3791/50775
6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
7. Zhang B, Radisic M. Organ-on-a-chip devices advance to market. Lab Chip. 2017. doi:10.1039/C6LC01554A
8. Wagner J, et al. Determination of bacterial endotoxin in pharmaceutical products. Pharmeuropa Bio. 2005. doi:10.1016/j.pharmbio.2005.01.001
9. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. Wiley. 2015. doi:10.1002/9781118873686